A 10-ml volume of phosphate-buffered saline was added to 0.5 g kidney tissue cut into 1-2 mm pieces and homogenized. The homogenate was centrifuged at 5,000 × g for 10 min at 4°C; the supernatant was collected and the levels of interleukin (IL)-6 (cat. no. MBS824560) and tumor necrosis factor (TNF)-α (cat. no. MBS175904) (both from MyBioSource); malondialdehyde (MDA; Cayman Chemical; cat. no. 10009055); and reduced to oxidized glutathione ratio (GSH/GSSG; Abcam; cat. no. ab138881) were analyzed.
Mbs175904
Manufactured by MyBioSource
Sourced in United States
The MBS175904 is a laboratory equipment product. It is designed for performing various scientific experiments and analyses. The core function of this product is to facilitate the execution of research and testing activities in a laboratory setting.
Automatically generated - may contain errors
2 protocols using mbs175904
1
Inflammatory Markers and Oxidative Stress in Kidney
2
Bioactive Compound Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Normal saline (0.9 % w/v) was prepared from the Samen® pharmacy factory, Iran. Xylazine, acepromazine, and ketamine were prepared from Alfasen, Woerden, Holland. Also, enzyme-linked immunosorbent assay (ELISA) kits, including VEGF, IL-6, TGF-β, IL-10, and TNF-α, were obtained from MyBioSource, USA with catalog number respectively; MBS3015758, MBS8244673, MBS2701296, MBS2707969, MBS175904. Dichloromethane (DCM) was purchased from Caledon, Canada, for HPLC grade. In addition, deionized water and Ethanol (96 %) were obtained from Abtin Co. (Iran), Kian Kaveh Azma Co. (Iran), and Taghtir Khorasan Co. (Iran), respectively. Furthermore, 6-gingerol was purchased from Golexir Pars ® Co., Iran, as an internal standard. Other chemicals and reagents were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!