Goat anti human igg fc fitc

Vero cells were infected with DENV1 TH-Sman (ATCC VR-1586), DENV2 New Guinea C (ATCC VR-1584), DENV3 H87 (BEI Resources) or DENV4 H241 (BEI Resources) at an MOI of 0.01. After 5 days, cells were harvested, then were fixed, permeabilized, and washed with Cytofix/Cytoperm and Cytoperm/Cytowash (BD Biosciences). Cells were incubated with sample containing human anti-DENV antibodies diluted in Cytoperm/Cytowash for 1 hr on ice, washed, and then stained with goat anti-human IgG Fc FITC (Abcam) for 1 hr on ice. After washing, cells were analyzed on a LSRII (BD Biosciences).

PLGA–COOH (50: 50, MW = 12.3 kDa) was purchased from Jinan Daigang Biomaterial Co. (Shandong, China). Paclitaxel was purchased from Meilun Bio (Dalian, China). Perfluoropentane (PFP), polyvinyl alcohol (PVA), IR780, N-(3-dimethyl-aminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 2-(N-morpholino) ethanesulfonic acid (MES monohydrate) and 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindocarbocyanine perchlorate (DiI), and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). PSMA monoclonal antibody was purchased from Cell Signaling Technology (USA). Goat anti-human IgG Fc (FITC) was purchased from Abcam (Shanghai, China). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Molecular Technology (Shanghai, China). RPMI-1640 mediumand fetal bovine serum (FBS) were purchased from Gibco (USA). All other reagents used in this work were of analytical grade and were used as received.