Rabbit anti phospho erk

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-phospho ERK is a primary antibody that specifically recognizes the phosphorylated form of Extracellular Signal-Regulated Kinase (ERK). It is used to detect the activation of the ERK signaling pathway in various experimental systems.

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Lab products found in correlation

Rabbit anti erk, by Cell Signaling Technology (1 mentions) Horseradish peroxidase linked secondary antibody, by Cell Signaling Technology (1 mentions) Rabbit anti β tubulin, by Cell Signaling Technology (1 mentions) Rabbit anti c jun, by Santa Cruz Biotechnology (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Rabbit anti β actin antibody, by Santa Cruz Biotechnology (1 mentions) Pd98059, by Cell Signaling Technology (1 mentions) Palmitate, by Merck Group (1 mentions)

2 protocols using rabbit anti phospho erk

Western Blot Analysis of KRAS Signaling

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mAb used: mouse anti-KRAS (1:1,000; Novus Biologicals), rabbit anti-cJun (1:1,000; Santa Cruz), rabbit anti-phospho ERK (1:1,000), rabbit anti-ERK (1:2,000), rabbit anti-GAPDH (1:5,000), rabbit anti-β-tubulin (1:5,000), and Horseradish peroxidase-linked secondary antibodies (1:10,000) (Cell Signaling). Bands were visualized by chemiluminescence using X-ray film.

Zdanov S., Mandapathil M., Abu Eid R., Adamson-Fadeyi S., Wilson W., Qian J., Carnie A., Tarasova N., Mkrtichyan M., Berzofsky J.A., Whiteside T.L, & Khleif S.N. (2016). Mutant KRAS conversion of conventional T cells into regulatory T cells. Cancer immunology research, 4(4), 354-365.

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Antibodies and Reagents for Cell Signaling

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Rabbit anti-phospho-JNK, rabbit anti-phospho-ERK, and rabbit anti-phospho-P38 monoclonal antibodies; rabbit anti-insulin receptor substrate (IRS)-2 and goat anti-PARP-1 polyclonal antibodies; and rabbit anti-β-actin antibody were purchased from Santa Cruz Biotechnology, CA, USA. PD98059 was from Cell Signaling, Beverly, MA, USA, and palmitate was obtained from Sigma, Louis, MO, USA. SB203580 and SP600125 were from Calbiochem, San Diego, CA, USA. Hoechst33342 was from Molecular Probes. Glucose (50%) was from Tianjing pharmaceutical of Group (Jiaozuo) Ltd., Tianjing province, China.

Zhou L., Cai X., Han X, & Ji L. (2014). P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research, 2014, 834528.

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