Cytokine levels in cell-free supernatants were assessed using standard sandwich ELISA for IL-5 and IL-13 (eBioscience) or using a commercially available ELISA kit for IL-9 (R & D Systems). For real-time PCR, RNA was isolated from lung tissue using an RNeasy Mini Kit (Qiagen, Valencia, CA) or from sort-purified cells using an RNeasy Micro Kit (Qiagen) according to the manufacturer instructions. RT-PCR or real-time PCR was performed on cDNA generated using a Superscript II reverse transcription kit (Life Technologies) using SYBR green master mix (Applied Biosystems) or Taqman Gene Expression Mastermix (Life Technologies) and commercially available primer sets (Qiagen Quantitect or Life Technologies Taqman primer assays). Samples were run on the ABI 7500 real-time PCR system (Life Technologies). RT-PCR products were run on a 3% agarose gel and visualized using a BioRad Gel Doc 1000 and Quantity One software.
Standard sandwich elisa for il 5 and il 13
Manufactured by Thermo Fisher Scientific
The Standard Sandwich ELISA for IL-5 and IL-13 is a laboratory assay used to measure the concentrations of the cytokines interleukin-5 (IL-5) and interleukin-13 (IL-13) in biological samples. The assay utilizes the enzyme-linked immunosorbent assay (ELISA) technique, which employs antibodies specific to IL-5 and IL-13 to detect and quantify their presence in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Gel doc 1000, by Bio-Rad (2 mentions)
Superscript 2 reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Elisa kit for il 9, by R&D Systems (2 mentions)
Taqman gene expression master mix, by Thermo Fisher Scientific (2 mentions)
Sybr green master mix, by Thermo Fisher Scientific (2 mentions)
Abi 7500 real time pcr system, by Thermo Fisher Scientific (2 mentions)
Rneasy mini kit, by Qiagen (2 mentions)
Rneasy micro kit, by Qiagen (2 mentions)
2 protocols using standard sandwich elisa for il 5 and il 13
1
Cytokine Detection in Lung Tissue
2
Cytokine Detection in Lung Tissue
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Cytokine levels in cell-free supernatants were assessed using standard sandwich ELISA for IL-5 and IL-13 (eBioscience) or using a commercially available ELISA kit for IL-9 (R & D Systems). For real-time PCR, RNA was isolated from lung tissue using an RNeasy Mini Kit (Qiagen, Valencia, CA) or from sort-purified cells using an RNeasy Micro Kit (Qiagen) according to the manufacturer instructions. RT-PCR or real-time PCR was performed on cDNA generated using a Superscript II reverse transcription kit (Life Technologies) using SYBR green master mix (Applied Biosystems) or Taqman Gene Expression Mastermix (Life Technologies) and commercially available primer sets (Qiagen Quantitect or Life Technologies Taqman primer assays). Samples were run on the ABI 7500 real-time PCR system (Life Technologies). RT-PCR products were run on a 3% agarose gel and visualized using a BioRad Gel Doc 1000 and Quantity One software.
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