Pd 10 desalting columns containing sephadex g 25 resin

Dicyclohexyl carbodiimide (DCC) (Cat # 802954), Dimethylformamide (DMF) (Cat # 103053), Poly (ethylene glycol) diacid (Cat # 843912), and anhydrous citric acid (Cat # 818707) were purchased from Merck. PD‐10 desalting columns containing Sephadex G‐25 resin (Cat # 170851) and Sephadex G15 (Cat # 170020) were purchased from GE Healthcare. N‐Hydroxysuccinimide (NHS) (Cat # 130672), RPMI 1640 (Cat # R8758), XTT (Cat # X4626), Hochest (Cat # B1155), and N‐Ethyl‐N′‐(3‐dimethylaminopropyl) carbodiimide (EDC) (Cat # E1769) were purchased from Sigma Aldrich. A549, L929, and NFS60 cell lines, rats, and mice were obtained from Iranian Genetic Resources Center and Pasteur Institute of Iran, respectively. G‐CSF was purchased from Cinnagen. Fetal bovine serum (FBS) (Cat # 10270) was purchased from GIBCO Invitrogen. Filter papers (Cat # DP 150‐150) and microplates (Cat # 3635) were purchased from Albet and corning, respectively. TSK G2000SWXL (Cat # 0008540), TSK G3000 SWXL (Cat # 0008541), and Guard (Cat # 0008543) were purchased from Tosoh. Quantikine ELISA kit for determination of G‐CSF (Cat # DCS50) was purchased from R&D systems.

The plasmid ompT_pET28a was a gift from Prof. Neil Kelleher (Northwestern University, Evanston IL, USA) while plasmid mut_ompT_pET28a was synthesized and sequenced by Bio Basic Inc (Singapore). E. coli Rosetta™ (DE3) cells with T7 RNA polymerase under the lac promoter were used for expressing wild type and mutant OmpTs as IBs and were purchased from Merck Millipore (Singapore). LB Broth was purchased from BD Difco (Franklin Lakes, NJ, USA). Kanamycin was purchased from Gibco by Life technologies (Now, ThermoFisher Scientific, Waltham, MA, USA). Isopropyl thio-β-D-galactopyranoside (IPTG) was purchased from Gold Biotechnology (St. Louis, MO, USA). N-Dodecyl-N, N-dimethyl-3-ammonio-1-propanesulfonate (DodMe₂NPrSO₃) and lipopolysaccharides (LPS) from E. coli 0111:B4 purified by phenol extraction, Corning^® Black, flat bottom 384-well plates were purchased from Sigma Aldrich (Singapore). Urea was purchased from Affymetrix USB (Santa Clara, CA, USA). PD-10 Desalting Columns containing Sephadex G-25 resin were purchased from GE Healthcare Life Sciences (Singapore). HiPPR™ Detergent Removal Spin Column was purchased from ThermoFisher Scientific (Singapore). Synthetic peptide, Abz-ARRA-Tyr (NO₂)-NH₂, Ac-GARKVG-NH2, and Ac-GARIIG-NH2 were purchased as lyophilized powder and certified >95% purity from Biomatik (Cambridge, ON, Canada).