Ph plcd1 gfp

Manufactured by Addgene

The PH-PLCD1-GFP is a plasmid construct that expresses a fusion protein of the PH domain of phospholipase C-delta 1 (PLCD1) and green fluorescent protein (GFP). This construct can be used to study the localization and dynamics of the PH domain of PLCD1 within cells.

Automatically generated - may contain errors

Lab products found in correlation

Ph btk gfp, by Addgene (2 mentions) Ph plcd1 r40l gfp, by Addgene (2 mentions) Mcherry clathrin lc 15, by Addgene (1 mentions) Gfp p4m sidm, by Addgene (1 mentions) Gfp p4m sidmx2, by Addgene (1 mentions) Pbgpa cmv gfp osbp ph domain, by Addgene (1 mentions) Mkate2 p2a apex2 tapp1 ph, by Addgene (1 mentions) Akt ph gfp, by Addgene (1 mentions)

2 protocols using ph plcd1 gfp

Plasmid-Mediated Cell Tracking and Manipulation

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasmids for tracking PI species were from Addgene. Specifically, mCherry-Clathrin LC-15 (Addgene #55019) [71 (link)]; PLCD1(PH)-mCherry (Addgene #36075) [72 (link)]; PH-PLCD1-GFP (Addgene #51407), PH-PLCD1(R40L)-GFP (Addgene #51408) [73 (link)]; pLentiLifeACT-EGFP BlastR (Addgene #84383) [74 (link)]; PH-Btk-GFP (Addgene #51463), GFP-PH-TAPP1 (Addgene #161985); and pLenti-EGFP-P4M-SidMx2 (Addgene #136997), pLenti-EGFP-2xFYVE (Addgene #136996) were gifts from Ken-Ichi Takemaru. Plasmids were also constructed by Vectorbuilder: VB200917 is a lenti-mCherry-shCLTC containing U6-driven shRNA sequence 5′-CGTGTTCTTGTAACCTTTATT-3′. VB200917 is a lenti-mCherry-shLuciferase containing U6-driven shRNA sequence 5′- ATGTTTACTACACTCGGATAT-3′ and was used to create lentivirus to infect MCF7, 4226, and MPE600 cell lines. Infected cells were sorted for mCherry using FACS as above and tested for engulfment. Human PIK3C2B-GFP fusion overexpression vector is a lentiviral CMV-driven plasmid from VectorBuilder. miRFP-713 (near-infrared fluorescent protein) overexpression vector is a lentiviral EFS-driven plasmid from VectorBuilder. 2xFYVE-domain-mCherry fusion protein is lentiviral plasmid from VectorBuilder. Lentiviral EFS-driven LYN11 mCherry fusion protein construct was also produced by VectorBuilder.

Frey W.D., Anderson A.Y., Lee H., Nguyen J.B., Cowles E.L., Lu H, & Jackson J.G. (2022). Phosphoinositide species and filamentous actin formation mediate engulfment by senescent tumor cells. PLOS Biology, 20(10), e3001858.

+ Open protocol

+ Expand

Plasmid Transfection and Infection in RAW264.7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following probes GFP-P4M-SidM (Addgene plasmid # 51469); GFP-P4M-SidMx2(Addgene plasmid #51472); PH-Akt-GFP (Addgene plasmid # 51465); PH-Akt(R25C)-GFP (Addgene plasmid # 51466) PH-PLCD1-GFP (Addgene plasmid # 51407); PH-PLCD1(R40L)-GFP (Addgene plasmid # 51408); PH-Btk-GFP (Addgene plasmid # 51463); PH-Btk(R28C)-GFP (Addgene plasmid # 51464) were gifts from Tamas Balla; GFP-EEA1 wt (Addgene plasmid # 42307) was a gift from Silvia Corvera; pBGPa-CMV-GFP-OSBP PH domain (Addgene plasmid # 58840) was a gift from Tim Levine & Sean Munro. mKate2-P2A-APEX2-TAPP1-PH (Addgene Plasmids #67662) was a gift was a gift from Rob Parton. Plasmids were isolated and test digested for confirmation. They were then introduced into RAW264.7 by nucleofection using reagents from Mirus Bio (Madison, Wisconsin). After seeding transfected cells and overnight culture, they were infected. Infection of transfectants were evaluated after 2 or 24hr.

Zhang N., Prasad S., Despointes C.E., Young J, & Kima P.E. (2018). Leishmania parasitophorous vacuole membranes display phosphoinositides that create conditions for continuous Akt activation and a target for miltefosine in Leishmania infections. Cellular microbiology, 20(11), e12889.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!