Substance p

Manufactured by Peptide Institute

Sourced in Japan

Substance P is a laboratory product that functions as a neurotransmitter and neuromodulator. It plays a role in the transmission of pain signals and the regulation of smooth muscle contractions.

Automatically generated - may contain errors

Lab products found in correlation

Aprepitant, by Cayman Chemical (1 mentions) Mithramycin, by Abcam (1 mentions) Pd98059, by Fujifilm (1 mentions) Recombinant human gm csf, by Bio-Techne (1 mentions) Sb203580, by Fujifilm (1 mentions) Birb796, by Axon Medchem (1 mentions) Leupeptin, by Peptide Institute (1 mentions) Glucagon, by Peptide Institute (1 mentions) Bovine insulin, by Merck Group (1 mentions) Tosyl l lysyl chloromethane hydrochloride, by Merck Group (1 mentions) 4 2 aminoethyl benzenesulfonyl fluoride hydrochloride, by Merck Group (1 mentions) Glp 1, by Peptide Institute (1 mentions) 3 4 dichloroisocoumarin, by Merck Group (1 mentions) Gly pro mca, by Peptide Institute (1 mentions) Quick taq hs dyemix, by Toyobo (1 mentions) α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Adenosine, by Merck Group (1 mentions) Recombinant mouse stem cell factor, by Thermo Fisher Scientific (1 mentions) Wortmannin, by Cayman Chemical (1 mentions)

4 protocols using substance p

Intracellular Signaling Pathways Regulating RANTES Production

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Human recombinant GM-CSF was obtained from Tocris Bioscience, Bristol, UK. Substance P (Peptide Institute Inc., Osaka, Japan), SB203580 (Wako, Kanagawa, Japan), aprepitant (Cayman Chemical, Ann Arbor, Michigan), PD98059 (Wako), BIRB796 (Axon Medchem, Groningen, Netherlands), U0216 (Promega Corporation, Madison, WI) and mithramycin (Abcam, Cambridge, UK) were employed to investigate the intracellular signaling pathways involved in RANTES production. The actions of all these reagents are summarized in Table 1.Table 1

Functional characteristics of chemical agents used.

Table 1

Chemical agents	Functions
Rottlerin	Protein kinase C inhibitor
TAPI-1	Disintegrin and metalloproteinase inhibitor
PDTC	The radical scavenger
Perifosine	Akt inhibitor
U0126	ERK1/2 inhibitor
Y-27632	ROCK inhibitor
Dynasore	Dynamin inhibitor
aprepitant	Substance P/NK-1 receptor antagonists

Akt: Protein kinase B; ROCK: Rho-associated coiled-coil forming kinase; NK-1: Neurokinin 1; ERK: Extracellular signal-regulated kinase.

Sakamoto A., Yamaguchi R., Yamaguchi R., Narahara S., Sugiuchi H, & Yamaguchi Y. (2018). Cross-talk between the transcription factor Sp1 and C/EBPβ modulates TGFβ1 production to negatively regulate the expression of chemokine RANTES. Heliyon, 4(7), e00679.

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Enzymatic Activity Characterization Protocol

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Restriction and DNA-modifying enzymes were purchased from Takara Bio and New England Biolabs. Quick Taq HS DyeMix came from Toyobo. Oligonucleotide primers were synthesized by FASMAC. Leupeptin, Gly-Pro-MCA, His-Ala-MCA, Lys-Ala-MCA, Phe-Met-MCA, [(2S, 3S)-3-carboxyoxirane-2-carbonyl]-l-leucine (4-guanidinobutyl) amide hemihydrate, GLP-1, GIP, glucagon, oxyntomodulin, and substance-P were purchased from Peptide Institute. Gly-Phe-MCA and Ser-Tyr-MCA were obtained from Bachem. Other substrates not commercially available, that is, His-Ala-MCA, Gly-Ala-MCA, Tyr-Ala-MCA, Phe-Leu-MCA, HAED-MCA, HAEG-MCA, HAEF-MCA, YAED-MCA, LDKA-MCA, TDKA-MCA, and HAEGTF-MCA, were synthesized by Scrum. CCL3 and CXCL6 were provided by GenScript Japan. Bovine insulin, tosyl-l-lysyl-chloromethane hydrochloride, aprotinin, sitagliptin phosphate monohydrate, 3,4-dichloroisocoumarin, 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride, and α-cyano-4-hydroxycinnamic acid were obtained from Sigma–Aldrich. Isoleucine thiazolidide hemifumarate (P32/98) was from FOCUS Biomolecules.

Ohara-Nemoto Y., Shimoyama Y., Ono T., Sarwar M.T., Nakasato M., Sasaki M, & Nemoto T.K. (2022). Expanded substrate specificity supported by P1′ and P2′ residues enables bacterial dipeptidyl-peptidase 7 to degrade bioactive peptides. The Journal of Biological Chemistry, 298(3), 101585.

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Mast Cell Activation Pathway Assay

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ATP, ADP, adenosine, αβmeATP, BzATP, UTP, UDP, UDP-G, 2,4-DNP human serum albumin (DNP-HSA), anti-DNP IgE (clone SPE-7), CP48/80, p-nitrophenyl N-acetyl-b-d-glucosaminide, fura-2-acetoxymethylester (fura-2AM), and ivermectin were from Sigma-Aldrich (St. Louis, MO, USA). Substance P and proadrenomedullin N-terminal 20 peptide were from Peptide Institute (Osaka, Japan). Allophycocyanin-conjugated rat anti-mouse CD117 (c-Kit) Ab (clone 2B8) was from BD Pharmingen (Franklin Lakes, NJ, USA). PE-conjugated mouse anti-mouse FcεRIα Ab (clone MAR-1) was from eBioscience (San Diego, CA). Recombinant mouse IL-3 and recombinant mouse stem cell factor were from PeproTech (Rocky Hill, NJ, USA). NP-1815-PX was provided by Nippon Chemiphar Co., Ltd. (Tokyo, Japan). SB203580 (p38 MAPK inhibitor) and wortmannin (PI3K inhibitor) were from Cayman Chemical (Ann Arbor, MI, USA). U0126 (MEK1/2 inhibitor) was from Cell Signaling Technology (Danvers, MA, USA). All other chemicals used were of reagent grade or of the highest quality available.

Yoshida K., Tanihara S., Miyashita Y., Obayashi K., Ito M.A., Yamamoto K., Imai T, & Matsuoka I. (2022). P2X4 receptor stimulation enhances MrgprB2-mediated mast cell activation and pseudoallergic reactions in mice. Scientific Reports, 12, 18613.

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Murine Peritoneal Macrophage Activation

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Peritoneal cells were collected from C57BL/6N mice, seeded at the concentration of 5Â10 5 /well in plates in RPMI-1640 complete medium supplemented with 10% fetal bovine serum and 100 IU/mL penicillin-streptomycin and incubated for 2 hours at 37 C and 5% CO 2 . The non-adherent cells were washed out, and the remaining adherent cells (>80% of macrophages) were incubated with or without substance P (1 mM; Peptide institute, Osaka, Japan), recombinant periostin (20 ng/mL; eBioscience, San Diego, CA), and/or RBC lysates (5% volume of total medium volume). After 24 hours, the cells were subjected to total RNA extraction or flow cytometric analysis. The RBC lysates were prepared as follows: murine whole blood was collected and centrifuged, and serum were removed. The blood was frosted and thawed twice.

Hashimoto T., Kursewicz C.D., Fayne R.A., Nanda S., Shah S.M., Nattkemper L., Yokozeki H, & Yosipovitch G. (2020). Mechanisms of Itch in Stasis Dermatitis: Significant Role of IL-31 from Macrophages. The Journal of investigative dermatology, 140(4).

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