The largest database of trusted experimental protocols

Ethylenediaminetetraacetic acid edta

Manufactured by Dojindo Laboratories
Sourced in Japan

Ethylenediaminetetraacetic acid (EDTA) is a chemical compound commonly used as a chelating agent. Its core function is to bind and chelate metal ions, forming stable complexes. EDTA is widely used in various applications, including water treatment, analytical chemistry, and medical procedures.

Automatically generated - may contain errors

4 protocols using ethylenediaminetetraacetic acid edta

1

Biotin-Avidin Surface Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
NeutrAvidin
and EZ-Link NHS-PEG4-biotin were
purchased from Thermo Fisher Scientific (Waltham, MA). Biotinylated
DNA and fluorescent-tagged oligonucleotides were purchased from Eurofin
Genomics (Tokyo, Japan). Manicure was purchased from Shiseido (Tokyo,
Japan). 3-Aminopropyltriethoxysilane (APTES) was purchased from Sigma
Aldrich (St. Louis, MO). Boric acid was purchased from Nacalai Tesque
(Kyoto, Japan). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased
from Dojindo (Kumamoto, Japan). A cover glass (18 mm × 18 mm,
no. 1S, 0.175 ± 0.015 mm thickness) and a slide glass (76 mm
× 26 mm, S-0314 Neo no. 2, 1.0–1.2 mm thickness) were
purchased from Matsunami Glass Industries (Osaka, Japan). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide
(EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). N-Hydroxysuccinimide (NHS) was purchased from Wako Pure
Chemical Industries (Osaka, Japan). All of the other reagents were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan),
unless otherwise specified, and used without further purification.
Type 1 ultrapure water (Milli-Q) was used in all of the experiments.
+ Open protocol
+ Expand
2

Reagents for Neurological Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), D(+)-glucose, ethanol, 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), magnesium chloride, N-ethylmaleimide (NEM), Nonidet P 40 (NP-40), potassium permanganate, sodium chloride, sodium dodecylsulfate (SDS) and tris(hydroxymethyl)aminomethane were purchased from Nacalai Tesque (Kyoto, Japan). Calcium chloride dehydrates, dantrolene sodium salt, l-glutamic acid, β-mercaptoethanol, neocuproine and TritonX-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid, acetone, caffeine, dithiothreitol (DTT) and potassium chloride were purchased from Wako Pure Chemicals (Osaka, Japan). Calcein-AM, 4′-6-diamidino-2-phenylindole (DAPI) solution, ethylenediaminetetraAcetic acid (EDTA), Hoechst33342, 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene (NOC7), 3-morpholinosydnonimine (SIN-1), were purchased from Dojindo (Kumamoto, Japan). Kainic acid (KA), (5S,10R)-(+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK-801) and N-methyl-d-aspartate (NMDA) were purchased from Tocris Bioscience (Bristol, UK).
+ Open protocol
+ Expand
3

Glucose-Responsive Hydrogel Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium alginate (194-13321), calcium chloride (090-00475), glucose (049-31165), acrylamide (016-00765) and mehylenebisacrylamide (134-02352) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Glucose-responsive monomer was presented by LG Chem Ltd. pH-responsive monomer, Fluorescein-5-Thiosemicarbazide (F-121), was purchased from Thermo Fisher Scientific. Disodium hydrogen phosphate (U0858) and sodium dihydrogen phosphate (U1886) were purchased from Sigma Aldrich. Photoinitiator (Irgacure 1173) was purchased from BASF. Ethylenediaminetetraacetic acid (EDTA) (341-01622) was purchased from Dojindo. Deionized (DI) water was purified with the Millipore Purification System.
+ Open protocol
+ Expand
4

Isolation and Preservation of H. marmoreus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fruiting bodies of H. marmoreus were purchased from a local market. Excised fruiting bodies were freezedried and stored at -30°C until use. Sheep blood was obtained from Nippon Bio-Supp Center, Tokyo, Japan. Phenylmethylsulfonyl fluoride (PMSF) and leupeptin were from MP Bio Japan, Tokyo, Japan, and ethylenediaminetetraacetic acid (EDTA) was from Dojindo Laboratories, Kumamoto, Japan. All chemicals used were of analytical grade.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!