Refill fluids contained PG (Thermofisher, Tustin CA, USA) and VG (Thermofisher, Tustin CA, USA). At 80% confluency, BEAS-2B cells were treated for 24 hours with nicotine, PG/VG, PV/VG with nicotine, or JUUL™ fluids. All treatments were diluted with BEGM culture medium to reach working concentrations. Liquid (−)- nicotine (Sigma-Aldrich, St. Louis, MO, USA) was diluted to reach final concentrations of 0.03 mg/mL or 0.3 mg/mL. PG/VG (30/70 ratio) was diluted to reach a final concentration of 0.5% in volume. Stock solutions of PG/VG with nicotine were made and then diluted with culture medium to 0.5% PG/VG with either 0.03 mg/mL nicotine or 0.3 mg/mL nicotine. JUUL™ “Virginia Tobacco” was diluted to 0.5%, which contains PG/VG at 0.5% concentration and nicotine at 0.3 mg/mL.
Liquid nicotine
Manufactured by Merck Group
Sourced in United States
Liquid (−)- nicotine is a chemical compound used in various laboratory applications. It is a clear, colorless liquid with a characteristic odor. The product is intended for research and scientific purposes only, and its core function is to serve as a reference standard or analytical tool in various experiments and analyses. No further details or interpretations regarding its intended use are provided.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using liquid nicotine
1
Nicotine and E-Cigarette Liquid Cytotoxicity
2
Radioligand Binding Assay Protocols
3
Amyloid-Beta Peptide Preparation and Characterization
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Unlabeled or uniformly 15N- or13C,15N-labeled Aβ(1–40) peptides were bought lyophilized from AlexoTech AB (Umeå, Sweden). The peptide samples were freshly dissolved and prepared before the measurements, according to previously published protocols61 (link). Liquid (-)-nicotine (Sigma-Aldrich) and acetate salts of Cd(II), Cr(III), Pb(II), and Pb(IV) (Sigma-Aldrich) were dissolved or diluted in 20 mM phosphate buffer. Naphthalene, phenanthrene, pyrene, and B[a]P were first dissolved in 50% DMSO and then diluted in 20 mM phosphate buffer. NaOH/HCl was used to adjust the pH of all stock solutions to 7.35.
4
Lung Cell Exposure to Nicotine and E-Cigarette Fluids
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Refill fluids contained PG (Thermofisher, Tustin CA, USA) and VG (Thermofisher, Tustin CA, USA). At 80% confluency, BEAS-2B cells were treated for 24 h with nicotine, PG/VG, PV/VG with nicotine, or JUUL fluids. All treatments were diluted with BEGM culture medium to reach working concentrations. Liquid (-)- nicotine (Sigma-Aldrich, St. Louis, MO, USA) was diluted to reach final concentrations of 0.03 mg/mL or 0.3 mg/mL. PG/VG (30/70 ratio) was diluted to reach a final concentration of 0.5% in volume. Stock solutions of PG/VG with nicotine were made and then diluted with culture medium to 0.5% PG/VG with either 0.03 mg/mL nicotine or 0.3 mg/mL nicotine. JUUL “Virginia Tobacco” was diluted to 0.5%, which contains PG/VG at 0.5% concentration and nicotine at 0.3 mg/mL.
5
Ligand Binding Assay for Nicotinic Receptors
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Care and Utilization Committee at the University of Colorado, Boulder and were conducted in accordance with the European Community Council Directive (2010/63/UE).
All of mice were bred at the Institute for Behavioral Genetics, and were housed at 22°C with lights on from 7 a.m. to 7 p.m. and allowed free access to food and water. The (±)-[ 3 H]-epibatidine ligand (Epi, specific activity: 66 Ci/mmol) was purchased from Perkin Elmer (Waltham, MA, USA); nonradioactive Epi, nicotine and α-bungarotoxin (α-Bgtx) were purchased from Tocris Bioscience (Bristol, UK). Liquid nicotine was purchased from Sigma/Aldrich (St. Louis, MO) and redistilled periodically. All other reagents (PMSF, proteases inhibithors, chemicals) were also from Sigma-Aldrich.
All of mice were bred at the Institute for Behavioral Genetics, and were housed at 22°C with lights on from 7 a.m. to 7 p.m. and allowed free access to food and water. The (±)-[ 3 H]-epibatidine ligand (Epi, specific activity: 66 Ci/mmol) was purchased from Perkin Elmer (Waltham, MA, USA); nonradioactive Epi, nicotine and α-bungarotoxin (α-Bgtx) were purchased from Tocris Bioscience (Bristol, UK). Liquid nicotine was purchased from Sigma/Aldrich (St. Louis, MO) and redistilled periodically. All other reagents (PMSF, proteases inhibithors, chemicals) were also from Sigma-Aldrich.
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