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Jem1400 plus electron microscope

Manufactured by Ametek
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The JEM1400-Plus Electron Microscope is a high-performance imaging system designed for advanced microscopy applications. It features a high-resolution electron beam and advanced imaging capabilities to provide detailed analysis of various samples.

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Lab products found in correlation

Formvar carbon coated 400 mesh copper grids, by Agar Scientific (1 mentions) Oneview camera, by Ametek (1 mentions)

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JEM1400-Plus (8 citations)

2 protocols using jem1400 plus electron microscope

1

Negative Staining for Electron Microscopy

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Electron microscopy grids were prepared by placing 4 μl of the sample onto formvar/carbon-coated 400-mesh copper grids (Agar Scientific Ltd, UK), followed by two washes with 0.22-μm-filtered milli-Q water. Then, 4 μl of uranyl acetate (2% w/v) was added to the grid and left for 30 s before blotting. EM projection images were collected using a JEOL JEM1400-Plus Electron Microscope operated at 80 kV equipped with a Gatan OneView camera (4k × 4k). Images were recorded at 25 fps with drift correction using GMS3.
Al-Hilaly Y.K., Hurt C., Rickard J.E., Harrington C.R., Storey J.M., Wischik C.M., Serpell L.C, & Siemer A.B. (2022). Solid-state NMR of paired helical filaments formed by the core tau fragment tau(297-391). Frontiers in Neuroscience, 16, 988074.
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2

Characterization of Nanoparticle Morphology

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Samples were drop-cast onto a 200 mesh copper grid with a formvar coating (TAAB). The sample was allowed to settle for 5 min before excess liquid was removed using filter paper. The grid was then loaded into a JEOL JEM-1400 Plus electron microscope and imaged using a GATAN OneView Camera. Dm3 were processed using ImageJ software (http://imagej.nih.gov/ij/) and nanoparticles were sized using the measure tool across the longest width of each nanoparticle. A total of 48 images were used (18 for the control, 30 for pMO-2137) arising across independent TEM sessions and biological replicates.
Capeness M.J., Imrie L., Mühlbauer L.F., Le Bihan T, & Horsfall L.E. (2019). Shotgun proteomic analysis of nanoparticle-synthesizing Desulfovibrio alaskensis in response to platinum and palladium. Microbiology, 165(12), 1282-1294.
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