Log In Sign up
The largest database of trusted experimental protocols

Ab652

Manufactured by Merck Group
Visit Supplier

Ab652 is a laboratory instrument used for the detection and quantification of specific proteins or other biomolecules in a sample. It utilizes antibody-based detection methods to provide accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Nb100 134, by Novus Biologicals (2 mentions) Fibrillarin, by Cell Signaling Technology (2 mentions) Nb300 666, by Abcam (2 mentions) Ab1344, by Abcam (2 mentions) Na934v, by Cytiva (2 mentions) Na931v, by Cytiva (2 mentions) Ab654, by Abcam (2 mentions) Ab113474, by Abcam (2 mentions) Mmv00, by R&D Systems (2 mentions)

2 protocols using ab652

1

Quantification of Retinal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Retinal samples were obtained as described above. Retinal lysate (20 µg) from three different animals or endothelial cells lysate (10 µg) were loaded on an SDS-PAGE gel and electroblotted onto a PVDF membrane. We used retinal nuclear extract (abcam, Ab113474, as per manufacturer’s protocol) to enrich Hif1a signals. After blocking, the membranes were incubated with antibodies against β-Actin (Sigma, A1978), Fibrillarin (Cell Signaling, 2639), Hif1a (Novus, NB100-134), and Glut1 (Novus, NB300-666 and Abcam, Ab652), Glut3 (Millipore, AB1344) and Glut4 (Abcam, ab654) overnight (1:1000 each). After washing, membranes were incubated with 1:10,000 horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Amersham, NA931V and NA934V) for one hour at room temperature. Densitometry was analyzed using ImageJ. Retinal Vegfa concentration was measured by ELISA (as per manual, MMV00, R&D Systems) and normalized by doing a Bradford to measure the total cell protein content of each samples.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
+ Open protocol
+ Expand
2

Quantification of Retinal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Retinal samples were obtained as described above. Retinal lysate (20 µg) from three different animals or endothelial cells lysate (10 µg) were loaded on an SDS-PAGE gel and electroblotted onto a PVDF membrane. We used retinal nuclear extract (abcam, Ab113474, as per manufacturer’s protocol) to enrich Hif1a signals. After blocking, the membranes were incubated with antibodies against β-Actin (Sigma, A1978), Fibrillarin (Cell Signaling, 2639), Hif1a (Novus, NB100-134), and Glut1 (Novus, NB300-666 and Abcam, Ab652), Glut3 (Millipore, AB1344) and Glut4 (Abcam, ab654) overnight (1:1000 each). After washing, membranes were incubated with 1:10,000 horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Amersham, NA931V and NA934V) for one hour at room temperature. Densitometry was analyzed using ImageJ. Retinal Vegfa concentration was measured by ELISA (as per manual, MMV00, R&D Systems) and normalized by doing a Bradford to measure the total cell protein content of each samples.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!