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2 protocols using rarres2 antibody

1

Protein Expression and Signaling Analysis

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A lysis buffer with 1% of SDS,10 mM of Tris HCl (pH 7.4), and supplemented with 1× Halt protease inhibitor cocktail and 1× Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific) was used. The primary antibodies used were: RARRES2 antibody (1:1 000, Abcam, ab72965); GAPDH antibody (1: 5 000, Santa Cruz Biotechnology, sc-47724), β-catenin antibody (1:1 000, Cell Signaling, #8480), phospho-β-catenin (Ser33/37/Thr41) antibody (1: 1 000, Cell signaling, #9561), phospho-p38 (Thr180/Tyr182) antibody (1:1 000, Cell Signaling, #4511), p38 antibody (1:1 000, Cell Signaling, #8690). Secondary antibodies used were goat anti-mouse IgG-horseradish peroxidase (HRP) and goat anti-rabbit IgG-HRP (1:5 000, Santa Cruz Biotechnology). Except for Western blots including multiple tissue samples, each Western blot experiments were repeated three times with independent sample sets.
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2

Protein Expression and Signaling Analysis

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A lysis buffer with 1% of SDS,10 mM of Tris HCl (pH 7.4), and supplemented with 1× Halt protease inhibitor cocktail and 1× Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific) was used. The primary antibodies used were: RARRES2 antibody (1:1 000, Abcam, ab72965); GAPDH antibody (1: 5 000, Santa Cruz Biotechnology, sc-47724), β-catenin antibody (1:1 000, Cell Signaling, #8480), phospho-β-catenin (Ser33/37/Thr41) antibody (1: 1 000, Cell signaling, #9561), phospho-p38 (Thr180/Tyr182) antibody (1:1 000, Cell Signaling, #4511), p38 antibody (1:1 000, Cell Signaling, #8690). Secondary antibodies used were goat anti-mouse IgG-horseradish peroxidase (HRP) and goat anti-rabbit IgG-HRP (1:5 000, Santa Cruz Biotechnology). Except for Western blots including multiple tissue samples, each Western blot experiments were repeated three times with independent sample sets.
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