1 m hydrochloric acid hcl

NaNO₃ was obtained from VWR international (Lutterworth, Leicestershire, UK). Sodium nitrite (NaNO₂), N-ethylmaleimide (NEM), Nonidet™ P 40 Substitute, mercuric chloride (HgCl₂) and 1 M hydrochloric acid (HCl) were obtained from Sigma-Aldrich Ltd. (Gillingham, UK). S-nitrosoglutathione (GSNO) was obtained from Enzo Life Sciences (Exeter, UK). Sodium iodide (NaI), potassium iodide (KI), iodine (I₂), acetic acid (99.5%), ethylenediaminetetraacetic acid (EDTA), vanadium (III) chloride (VCl₃), potassium ferricyanide, sulfanilamide and methanol were obtained from Fisher Scientific (Loughborough, Leicestershire, UK). Lithium-heparin tubes were obtained from Becton Dickinson UK Ltd. (Wokingham, UK). Cannulas were obtained from Becton Dickinson Insyte-WTM (Madrid, Spain). NO₃⁻-rich beetroot juice (BR; beetroot juice containing 6.4 mmol of NO₃⁻ per 70 ml; “Beet It Sport”) and NO₃⁻-depleted beetroot juice (PL; beetroot juice containing 0.04 mmol of NO₃⁻ per 70 ml) were obtained as gifts from James White Drinks Ltd. (Ipswich, UK). The reagents and buffers were made up in ultrapure water (18.2 MΩ/cm) unless otherwise indicated.

α1-acid glycoprotein (AGP) C.A.T. #(66455-27-4), PBS, high pure chitosan, and lipopolysaccharide (LPS) from E. coli 026:B6 were from Sigma (L8274-10MG). The QuantiPro TM BCA Assay kit was from Sigma Aldrich; St. Louis, MO, USA (QPBCA-1KA). High purity chitosan (deacetylation degree over 75–85% mol and viscosimetric molecular weight 50–190 kDa), sodium triphosphate pentabasic (TPP), 1 M hydrochloric acid (HCl), and sodium hydroxide (NaOH) were obtained from Sigma-Aldrich (Gillingham, UK). Milli-Q water was used in all synthetic experiments. Hyaluronic acid (HA; weight average molecular weight ≈200 kDa) was purchased from Medipol SA; Lausanne, Switzerland. Regenerated cellulose (RC) dialysis membrane (MWCO 10kDa) was obtained from Spectra Por, Spectrum Laboratories Inc.; Los Angeles, CA, USA.