STT and FLX were obtained from Sigma-Aldrich (St Louis, MO, USA). Reference standards were purchased from Clearsynth Ltd. (Mumbai, India). Kits for the determination of biochemical parameters (cholesterol, triglycerides) were obtained from Human Diagnostics (Wiesbaden, Germany). Acetonitrile of liquid chromatography/mass spectrometry (LCMS) grade and methanol (purity 99.9%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Liquid ammonia (about 25% NH3, analytical grade), orthophosphoric acid (analytical grade), and ammonium formate (99.0%, LCMS grade) were purchased from Sigma-Aldrich. Milli-Q water used for the analysis was prepared in house by using Milli-Q water purification unit procured from EMD Millipore (Billerica, MA, USA). The blank control plasma containing K3 EDTA was collected from animal house of Prince Sultan Military Medical City (Riyadh, Saudi Arabia) and stored below −70°C in the deep freezer. All other reagents used for liquid chromatography/tandem mass spectrometry (LCMSMS) analysis were obtained from Merck Scientific (Kenilworth, NJ, USA).
Milli q water purification unit
Manufactured by Merck Group
Sourced in United States
The Milli-Q water purification unit is a laboratory equipment designed to produce high-quality, ultrapure water. The system utilizes a combination of filtration, deionization, and other purification techniques to remove impurities and contaminants from the input water, resulting in a consistent supply of purified water suitable for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (3 mentions)
Sodium acetate, by Merck Group (2 mentions)
Anhydrous sodium carbonate, by Thermo Fisher Scientific (2 mentions)
Hexane, by Thermo Fisher Scientific (2 mentions)
Ethanol, by Thermo Fisher Scientific (2 mentions)
Chloroform, by Thermo Fisher Scientific (2 mentions)
Acetone, by Merck Group (2 mentions)
Linoleic acid, by Thermo Fisher Scientific (2 mentions)
Gallic acid, by Thermo Fisher Scientific (2 mentions)
Potassium persulfate, by Thermo Fisher Scientific (2 mentions)
Trolox, by Thermo Fisher Scientific (2 mentions)
Folin ciocalteu s reagent, by Merck Group (2 mentions)
Iron 3 chloride anhydrous, by Thermo Fisher Scientific (2 mentions)
Tween 40, by Thermo Fisher Scientific (2 mentions)
2 2 azinodi 3 ethyl benzthiazoline sulfonate, by Merck Group (2 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Liquid ammonia, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
9 protocols using milli q water purification unit
1
Quantitative Analysis of Biomarkers
2
Hyaluronic Acid Characterization Protocol
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HA (CASRN: 1415–93-6, Sigma-Aldrich, technical), sulfuric acid (H2SO4, Sigma-Aldrich, 98%), sodium hydroxide (NaOH, Panreac, 0.25 M), potassium iodide (KI, Panreac, 99%), sodium phosphate monobasic (H2O4PNa·H2O, Fisher, 98%) and sodium phosphate dibasic heptahydrate (HNa2O4P·H2O, Fisher, 99%) were used as received. Deionised water was supplied by a Milli-Q® water purification unit supplied by Merck.
3
Cultivating Fresh Morel Mushrooms
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Fresh morel mushrooms from two different cultivation methods were collected during their fruiting period. This matrix was first prepared by wheat (40%), mixed wood chips (30%), rice husk (20%), and humus (10%). Then, an extra lime (1%), gypsum (2%), potassium dihydrogen phosphate (0.1%), and sour jujube kernel (5%) were added. In addition, the matrix of control group has the same components except that no sour jujube kernel is added. Formic acid and ammonium acetate were commercially available from Sigma-Aldrich (Madrid, Spain). Methanol and acetonitrile were obtained from ANPEL (Shanghai, China). A Milli-Q water purification unit (Millipore, Millipore, MA, USA) was used throughout the study. The chemical reagents and solvents used in this product have been subjected to high performance liquid chromatography or analysis.
4
Polymer Blends and Surface Characterization
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Unrecycled PP, AMPPLEO 1020 GA (0.94 g/cm3, 20 g/10 min: MFI) (Philadelphia, PA, USA) was purchased from Braskem, and recycled PP Eco Meplen IC M20 BK (0.90 g/cm3, 50 g/10 min: MFI) was purchased from MEPOL S.r.l. (Treviso, Italy), Wood Fiber was obtained locally (softwood). Ultrapure water was dispensed by a MilliQ water purification unit (Millipore Corporation, Danvers, MA, USA). Electrolyte solutions for the surface Zeta Potential analysis were prepared from KCl p.a., 0.1 moL/L HCl and 0.1 moL/L KOH, purchased from Carl Roth, Germany.
5
Chitosan-Based Nanoparticle Formulation
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Chitosan (high molecular weight, ≥75% deacetylated) was purchased from Fluka Chemie (Buchs, Switzerland), Flavourzyme® was purchased from Novozymes (Bagsværd, Copenhagen, Denmark), and sodium tripolyphosphate (TPP) and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). All other chemicals were of analytical grade. The water used was purified using a Milli Q water purification unit (Millipore Corp., Bedford, MA, USA).
6
Halochromic Compounds in Modeling Oils
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Chemicals and materials 1-octanol (95%, Fisher Scientific, Canada) was selected as a modeling oil because the partition coefficients of many chemicals in octanol and water are available in the literature.
The halochromic compounds were Bromocresol green (BG, yellow to blue at pH 3.8 to 5.4), bromocresol purple (BP, yellow to purple at pH 5.2 to 6.8). All halochromic compounds were purchased from Fisher Scientific (Canada). Several different types of acids in oils were tested, including citric acid (99.5%), gallic acid (97.5%), 4-hydroxybenozic acid (99%), benzoic acid (99.5%) and trimesic acid (99%), all purchased from Sigma-Alderich (Canada). Acetic acid (99.7%) and heptanoic acid (98%) were purchased from Fisher Scientific (Canada). The LgP and dissociation constant (pKa) of each acid are shown in Table 1. Water was obtained from Milli-Q water purification unit (Millipore Corporation, Boston, MA, USA).
The halochromic compounds were Bromocresol green (BG, yellow to blue at pH 3.8 to 5.4), bromocresol purple (BP, yellow to purple at pH 5.2 to 6.8). All halochromic compounds were purchased from Fisher Scientific (Canada). Several different types of acids in oils were tested, including citric acid (99.5%), gallic acid (97.5%), 4-hydroxybenozic acid (99%), benzoic acid (99.5%) and trimesic acid (99%), all purchased from Sigma-Alderich (Canada). Acetic acid (99.7%) and heptanoic acid (98%) were purchased from Fisher Scientific (Canada). The LgP and dissociation constant (pKa) of each acid are shown in Table 1. Water was obtained from Milli-Q water purification unit (Millipore Corporation, Boston, MA, USA).
7
Analytical Reagents and Solvents Procurement
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All the chemicals and reagents were of analytical grades. Ethanol, hexane, gallic acid, linoleic acid, Trolox, Tween 40, sodium carbonate anhydrous, chloroform, iron (III) chloride anhydrous, and potassium persulfate were purchased from Fisher Scientifi c Co. (Fisher Scientifi c, Loughborough, UK). Acetone, mEthanol, Folin-Ciocalteu's reagent, and 2,2'-azinodi[3-ethyl-benzthiazoline sulfonate] (ABTS) were from Merck KGaA (Lichrosolv, Darmstadt, Germany), while 2,2-diphenyl-1-picryhydrazyl (DPPH), β-carotene, butylated hydroxy anisole (BHA), acetic acid, and sodium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Distilled water used was obtained from a Milli-Q water purifi cation unit (Millipore, Milford, MA, USA).
8
Porcine Pancreatic Lipase Assay
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Lipase from a porcine pancreas Type II (a crude preparation which contains the lipase-colipase complex as well as protease and amylase activity, Cat No 9001--62-1) and bile salts (containing a mixture of sodium salts of cholic acid (~50%) and deoxycholic acid (~50%), Cat No 48305) were obtained from Sigma Aldrich (Saint Louis, Missouri, USA). Distilled water was obtained from a Milli-Q water purification unit (Millipore, Milford, USA). All other chemicals and reagents were of analytical grade.
9
Antioxidant Activity Characterization
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All the chemicals and reagents were of analytical grade. Ethanol, hexane, gallic acid, linoleic acid, Trolox, Tween 40, sodium carbonate anhydrous, chloroform, iron(III) chloride anhydrous, and potassium persulfate were purchased from Fisher Scientifi c Co. (Fisher Scientifi c, Loughborough, UK). Acetone, mEthanol, Folin-Ciocalteu's reagent, and 2,2'-azinodi[3-ethyl-benzthiazoline sulfonate] (ABTS) were from Merck KGaA (Lichrosolv, Darmstadt, Germany), while 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene, butylated hydroxy anisole (BHA), acetic acid, and sodium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Distilled water was obtained from a Milli-Q water purifi cation unit (Millipore, Milford, MA, USA).
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