Am2290

Manufactured by Thermo Fisher Scientific

Sourced in United States

The AM2290 is a laboratory instrument designed for performing ultra-low temperature measurements. It is a cryogenic temperature controller capable of precisely regulating and monitoring temperatures down to millikelvin levels. The device is equipped with features to enable accurate temperature control and data acquisition in cryogenic research and applications.

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Lab products found in correlation

Am2272, by Thermo Fisher Scientific (3 mentions) C18 ziptip, by Merck Group (1 mentions) Quantigene viewrna ish cell assay kit, by Thermo Fisher Scientific (1 mentions) Ne pertm nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)

4 protocols using am2290

RNaseIII Cleavage Assay for In vitro Transcripts

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In vitro transcribed RNAs were subjected to RNaseIII cleavage assay as follow: 10 pmol of RNA were used in 20 μl reaction volume containing 1× enzyme buffer (AM2290; Ambion). Reactions were pre-incubated at 37°C for 5 min, followed by the addition of 0.5 U of E. coli RNaseIII (AM2290; Ambion) and further incubation at 37°C for up to 15 min. Reactions were stopped by the addition 5 mM EDTA and purified with ZipTip C18 (10 μl bed; Millipore). Cleavage products were separated on denaturing urea gel (15% TBU; Novex) which was then stained with 1× SYBRGold and exposed to a PhosphorImager screen.

Morra R., Pratama F., Butterfield T., Tomazetto G., Young K., Lopez R, & Dixon N. (2023). arfA antisense RNA regulates MscL excretory activity. Life Science Alliance, 6(6), e202301954.

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QuantiGene ViewRNA FISH Assay Protocol

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To detect EGOT and pre-ITPR1 mRNAs, we used the QuantiGene® ViewRNA ISH Cell Assay Kit (Catalogue Number QVC0001, Thermo Fisher) to perform the QuantiGene ViewRNA FISH assay according to the manufacturer’s protocol. EGOT, ITPR1 and ACTB (as control) hybridization was carried out using cy3, cy5, and 488-nm DNA-oligonucleotide probes in a moist chamber, respectively. After digestion with a working protease solution, slides were incubated with RNase III (AM2290, Life Technologies, USA) or RNase A (AM2272, Life Technologies) for 2 h if RNase enzymatic activity was to be determined. Standard immunofluorescence and imaging were performed by confocal microscopy. The details of the probe sets and corresponding gene sequences are provided in Additional file 1: Table S2.

Xu S., Wang P., Zhang J., Wu H., Sui S., Zhang J., Wang Q., Qiao K., Yang W., Xu H, & Pang D. (2019). Ai-lncRNA EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNA-RNA and RNA-protein interactions in human cancer. Molecular Cancer, 18, 89.

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FISH Assay for TINCR Detection

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ViewRNA^®Probe (Catalogue Number VA1-3016120, Santa Clara) was purchased to perform FISH assay according to the manufacturer’s protocol. TINCR hybridization was carried out in a moist chamber. After digestion with a working protease solution, slides were incubated with RNase III (AM2290, Life Technologies, USA) or RNase A (AM2272, Life Technologies) for 2 h. Standard immunofluorescence and imaging was performed by confocal microscopy.

Wang Q., Liu J., You Z., Yin Y., Liu L., Kang Y., Li S., Ning S., Li H., Gong Y., Xu S, & Pang D. (2021). LncRNA TINCR favors tumorigenesis via STAT3–TINCR–EGFR-feedback loop by recruiting DNMT1 and acting as a competing endogenous RNA in human breast cancer. Cell Death & Disease, 12(1), 83.

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Nuclear-Cytoplasmic Fractionation and FISH

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Nuclear/cytoplasmic fractions were isolated using the NE-PERTM nuclear and cytoplasmic extraction reagents (Catalog number 78835; Thermo Fisher) according to the manufacturer's protocol. Cytoplasmic and nuclear fractions were divided for RNA extraction. The GAPDH and U1 enzymes were used as qRT-PCR markers for the cytoplasmic and nuclear RNAs, respectively. RNA uorescent in situ hybridization (RNA-FISH) ViewRNA®Probe (Catalogue Number VA1-3016120, Santa Clara) was purchased to perform Fluorescence in situ hybridization (FISH) assay according to the manufacturer's protocol. LINC00036 hybridization was carried out in a moist chamber. After digestion with a working protease solution, slides were incubated with RNase III (AM2290, Life Technologies, USA) or RNase A (AM2272, Life Technologies) for 2 h. Standard immuno uorescence and imaging were performed by confocal microscopy.

Xu S., Wan L., Wang Q., Yin H., Qiao K., Li S., Zhang J., Wu H., Shen M., Ning S., & Pang D. (2021). C-MYC inducible onco-lncRNA LINC00036 acting as EGFR mRNA stabilizer via RNA-protein and RNA-RNA interactions decreases the sensitivity of gefitinib in human cancer.

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