To quantitatively analyze the intracellular ATP, 50 mg mycelia were collected and ground to a powder in liquid nitrogen. The ATP content was measured using the ATP assay kit (Beyotime, China). Luminescence was quantified using the Synergy H1 multidetection microplate reader (BioTek, USA) at wavelengths between 520 and 620 nm. The ATP content was calculated on the basis of a standard curve and was normalized against the protein concentrations.
The MMP was analyzed using the JC-1 mitochondrial membrane potential detection kit (Beyotime, China). Mycelia were collected by centrifugation, resuspended in the JC-1 working solution, and incubated at 37°C for 30 min. The mycelia were then washed and analyzed for fluorescence, which was quantified according to the manufacturer’s instructions.
The MMP was analyzed using the JC-1 mitochondrial membrane potential detection kit (Beyotime, China). Mycelia were collected by centrifugation, resuspended in the JC-1 working solution, and incubated at 37°C for 30 min. The mycelia were then washed and analyzed for fluorescence, which was quantified according to the manufacturer’s instructions.