Glucose 6 phosphate (g6p)

HO-1 activity was assessed according to the method of Basu et al. [35 (link)]. Briefly, a reaction mixture containing 2 mM glucose-6-phosphate (Santa Cruz, USA), 1 U glucose-6-phosphate dehydrogenase, 1 mM NADPH, 25 μM hemin, 2 mg of rat liver cytosolic protein (as biliverdin reductase source), and 100 mM potassium phosphate buffer (pH 7.4) was incubated with 600 μg of brain microsomal protein. The final volume was adjusted to 400 μl with potassium phosphate buffer and incubated for 1 h at 37 °C in the dark. Finally, the reaction was terminated by putting the tubes on ice for 2 min. HO-1 activity was determined via measuring bilirubin concentration as the difference in absorbance between 464 and 530 nm. HO-1 activity was expressed in pmol bilirubin/ mg protein/h.

D-fructose (Product No. F0127), glyceraldehyde-3-phosphate (Product No. G5251), 3-phosphoglycerate (Product No. P8877), 2-phosphoglycerate (Product No. 79480), phosphoenolpyruvate (Product No. P7002), acetate (Product No. S2889) and pyruvate (Product No. P2256) are from Sigma-Aldrich. Glucose-6-phosphate (Product No. SC-221489A) is from Santa Cruz Biotechnology. Glycerol (Product No. 3783.2) is from Carl Roth. All chemicals were tested for their purity to exclude contamination by glucose.