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Brain heart infusion agar

Manufactured by Carl Roth
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Sourced in Germany, United Kingdom, India

Brain heart infusion agar is a culture medium used for the growth and isolation of a wide range of microorganisms, including bacteria and fungi. It provides the necessary nutrients and growth factors for the cultivation of fastidious microorganisms.

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Lab products found in correlation

Ringer s solution, by Merck Group (1 mentions) Sodium fumarate, by Merck Group (1 mentions) Paraquat dichloride hydrate, by Merck Group (1 mentions) Benzyl viologen dichloride, by Merck Group (1 mentions) Catalase from bovine liver, by Merck Group (1 mentions) Hemin, by Merck Group (1 mentions) Metronidazole, by Merck Group (1 mentions) Xanthine, by Merck Group (1 mentions) Anaerocult a, by Merck Group (1 mentions) Tris hcl, by Merck Group (1 mentions) Vitamin k1, by Carl Roth (1 mentions) Brain heart infusion broth, by Carl Roth (1 mentions) Triton x 100, by Merck Group (1 mentions) Cytochrome c, by Merck Group (1 mentions) β mercaptoethanol, by Merck Group (1 mentions) Nadph, by Merck Group (1 mentions) Oxaloacetate, by Merck Group (1 mentions) Pyruvic acid, by Merck Group (1 mentions) Coenzyme a, by Merck Group (1 mentions) Xanthine oxidase, by Merck Group (1 mentions)

3 protocols using brain heart infusion agar

1

Quantifying Microbial Counts in Chicken

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Samples were prepared for microbiological analysis as already described in Höll et al., 2016 [7 (link)], with slight modifications: In a typical analysis, a total of 70 g of chicken strips were weighed in a sample bag (VWR International, Darmstadt, Germany) and homogenized for 120 s with 50 mL Ringer’s solution (Merck KGaA, Darmstadt, Germany) in a stomacher (LabBlender400, Gemini BV, Apeldoorn, Netherlands). A tenfold dilution series of chicken homogenate was prepared with Ringer’s solution. An amount of 100 μL of each dilution was later spread onto the brain heart infusion agar (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) using sterile glass beads. After incubating the plates aerobically at 30 °C for 3 days, the number of colony-forming units on the plates were counted and the units per gram sample (CFU/g) were calculated.
Dold J., Kehr C., Hollmann C, & Langowski H.C. (2022). Non-Destructive Measuring Systems for the Evaluation of High Oxygen Stored Poultry: Development of Headspace Gas Composition, Sensory and Microbiological Spoilage. Foods, 11(4), 592.
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2

Anaerobic Microbial Growth Enablers

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Wilkins-Chalgren anaerobe agar (WC) was purchased from Oxoid (Basingstoke, England), and Brain Heart Infusion Broth (BHI), Brain Heart Infusion Agar, and vitamin K1 were purchased from Carl Roth (Karlsruhe, Germany). Hemin, metronidazole, NADH, NADPH, cytochrome c, benzyl viologen dichloride, catalase from bovine liver, paraquat dichloride hydrate, Tris/HCl, Triton X-100, xanthine, pyruvic acid, oxaloacetate, sodium fumarate, β-mercaptoethanol, and Coenzyme A were all purchased from Sigma-Aldrich (St. Luis, USA). Potassium dihydrogen phosphate (KH2PO4), hydrogen peroxide, sodium dithionite, xanthine oxidase, ethylenediaminetetraacetic acid (EDTA), sodium chloride, and Anaerocult A were purchased from Merck (Darmstadt, Germany). Etests were purchased from bioMérieux (Marcy-l'Étoile, France).
Paunkov A., Sóki J, & Leitsch D. (2022). Modulation of Iron Import and Metronidazole Resistance in Bacteroides fragilis Harboring a nimA Gene. Frontiers in Microbiology, 13, 898453.
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3

Fungal Identification Protocol from Clinical Isolates

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All positive tubes were also sub-cultured onto Sabouraud Dextrose Agar (Central Drug House (CHD), Ltd, of India) and Brain Heart Infusion Agar (Carl Roth, Ltd, of Germany) for fungal growth. Culture plates were examined twice a week for any fungal growth for about four weeks. Cultures of mycelia fungi (molds) were identified by examining macroscopic and microscopic characteristics of their colony. Texture, rate of growth, topography and pigmentation of the front and the reverse side of the culture were employed. For the macroscopic identification. Microscopic identification of mold isolates was performed by placing pieces of a colony from Sabouraud Dextrose Agar to clean microscopic slide and staining with lacto phenol cotton blue. After placing a cover slip, each preparation was observed microscopically.
Addise D., Bitew A., Yaregal Z., Yenew B., Mollalign H., Diriba G, & Kebede A. (2016). Effect of 1.5% sodium hydroxide final concentration on recovery rate of Mycobacterial Species and decontamination of other Bacterial and Fungal contaminants on sputum. Ethiopian journal of public health and nutrition, 1(1), 57-67.
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