Oasis hlb 200 mg

Antibiotics concentrations were quantified for soil, runoff, and manure slurry samples. Target compounds, chosen based on the usage data from the facility operator and expected persistence or chemical properties of the parent compounds, were chlortetracycline, lincomycin, tiamulin, penicillin G and its degradation product penillic acid. Analytes were extracted and purified from liquid and solid samples and concentrated using polymeric (Oasis HLB 200 mg, Waters Corporation, Milford, MA) solid phase extraction. Oleandomycin was used as surrogate, while roxithromycin, doxycycline, and penicillin V were used as internal standards. Results of method validation indicated detection limits ranged between 0.005 and 0.014 μg/L in water, and between 0.14 and 0.68 ng/g in soil. Concentrated extracts were analyzed on a Waters Quattro Micro triple quadrupole mass spectrometer coupled with a Waters 2695 high pressure liquid chromatography (HPLC). Additional detail about the processing and analytical methods is reported in the Supplementary information file. Surrogate recovery averaged 107 ± 62% in runoff samples and 80 ± 35% in soil samples. Method detection limits for the analytes in various sample matrices are reported in Table S2.

For the cell experiments, quinoa aglycones were extracted in order to assess the isolated effects on the intestinal transport. Half a gram of quinoa flour was weighed into a 50 mL centrifuge tube and suspended in 9 mL 50% methanol (Fluka, Steinheim, Germany) in MilliQ water (v/v) for 1 h at room temperature while shaking. The suspension was centrifuged at 4030 g for 5 min and subsequently mixed with 2 mL HCl (Emsure, Merch, Kenilworth, NJ, US) for hydrolysis at 80 °C for 90 min. Solid phase extraction (SPE) cartridges (Oasis HLB 200 mg, Waters, Etten-Leur, The Netherlands) were used for sample purification using a vacuum manifold. Columns were activated with 3 mL methanol followed by 3 mL MilliQ water. One mL of the quinoa extract was added to the columns followed by washing with 5 mL MilliQ water. The quinoa aglycones were eluted from the columns using 3 mL methanol. The eluates were dried under a steam of nitrogen at 50 °C for approximately 30 min.
Quantification of quinoa aglycones were assessed by HPLC-ESI-QTOF-MS.