Immunocytochemistry was performed on whole tadpoles and subsequently the hearts were dissected, mounted in 12 μl CyGEL Sustain (biostatus) and viewed using Zeiss LSM5-Pascal or LSM710 confocal microscopes. Confocal images of whole hearts captured 2 μm deep optical sections. Both the ventricle myocardial wall and also deeper trabecular layers were assessed by scanning different depths. Images of cardiomyocytes and myofibrils were 1 μm optical sections, at a depth 1–2 μm below the outer (apical) myocardial surface. Antibodies used were Adprhl1, Myosin A4.1025 (DSHB) and phospho-Histone H3(Ser10) 3H10 (Sigma), along with fluorescent dye-conjugated secondary antibodies (Jackson ImmunoResearch). Atto 633-conjugated phalloidin (Sigma) stained actin filaments. Dying cells were visualized with the ApopTag® Red In Situ Apoptosis Detection kit (Sigma).
Atto 633 conjugated phalloidin
Manufactured by Merck Group
Atto 633-conjugated phalloidin is a fluorescent probe that binds specifically to filamentous actin (F-actin) in cells. It is a useful tool for visualizing and studying the cytoskeleton in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (4 mentions)
Lsm 5 pascal, by Zeiss (2 mentions)
Phospho histone h3 ser10 3h10, by Merck Group (2 mentions)
Fluorescent dye conjugated secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Apoptag red in situ apoptosis detection kit, by Merck Group (2 mentions)
Cygel sustain, by Biostatus (2 mentions)
2 protocols using atto 633 conjugated phalloidin
1
Imaging Cardiomyocytes in Developing Tadpoles
2
Immunocytochemistry of Tadpole Hearts
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Immunocytochemistry was performed on whole tadpoles and subsequently the hearts were dissected, mounted in 12 µl CyGEL Sustain (biostatus) and viewed using Zeiss LSM5-Pascal or LSM710 confocal microscopes. Confocal images of whole hearts captured 2 µm deep optical sections. Both the ventricle myocardial wall and also deeper trabecular layers were assessed by scanning different depths. Images of cardiomyocytes and myofibrils were 1 µm optical sections, at a depth 1-2 µm below the outer (apical) myocardial surface. Antibodies used were Adprhl1, Myosin A4.1025 (DSHB) and phospho-Histone H3(Ser10) 3H10 (Sigma), along with fluorescent dyeconjugated secondary antibodies (Jackson ImmunoResearch). Atto 633-conjugated phalloidin (Sigma) stained actin filaments. Dying cells were visualized with the ApopTag ® Red In Situ Apoptosis Detection kit (Sigma).
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