Brca1 primary antibody

Manufactured by Santa Cruz Biotechnology

The BRCA1 primary antibody is a research-use only product designed to detect the BRCA1 protein in various sample types. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of BRCA1. The BRCA1 protein is a tumor suppressor involved in DNA repair and cell cycle regulation.

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Lab products found in correlation

Amaxa cell line nucleofector kit 5, by Lonza (1 mentions) Crispr cas9 knockout ko plasmids, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg h l alexafluor 594, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BRCA1 (61 citations) BRCA1 antibody (3 citations)

1 protocol using brca1 primary antibody

CRISPR/Cas9 Mediated Modulation of BRCA1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

CRISPR/Cas9 mediated knockout was done to suppress the expression of endogenous BRCA1 in primary human CD4+T helper cells isolated from control subjects, NSCLC and CAD patients. Transfection was performed using CRISPR/Cas9 knock-out (KO) plasmids (Santa Cruz) and their control plasmid (Santa Cruz) utilizing all the supplied reagents as per the manufacturer's protocol. Transfection was done successfully which was verified by flow cytometry and transfection efficiency was checked by GFP staining. BRCA1 primary antibody (Santa Cruz) and secondary antibody, Goat anti-Rabbit IgG (H+L) APC (Santa Cruz) was used for this purpose. Human tagged ORF clone of BRCA1 gene (Origene) was used to overexpress the BRCA1 gene using Amaxa^TM Cell Line Nucleofector^TM Kit V (Lonza) and Amaxa nucleofector 2b. After that flow cytometry was done using BRCA1 primary antibody (Santa Cruz) and a secondary antibody, Goat anti-Rabbit IgG (H+L) Alexa Fluor 594 (Invitrogen) to verify successful overexpression of the particular gene [15] (link).

Rakshit S., Sunny J.S., George M., Hanna L.E, & Sarkar K. (2021). R-loop modulated epigenetic regulation in T helper cells mechanistically associates coronary artery disease and non-small cell lung cancer. Translational Oncology, 14(10), 101189.

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