Striatum samples were homogenized by sonication in SDS buffer (10%). After determining the dynamic range of detection for each antibody, 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly, membranes were incubated overnight at 4°C with primary antibodies of interest anti-: TH (Cell Signaling; s58844), mTOR (Cell Signaling; s2983), p-mTOR (Cell Signaling; s5536), iNOS (Cell Signaling; s13120), MPC1 (Cell Signaling; s14462), pyruvate dehydrogenase (Cell Signaling; s3205), p-pyruvate dehydrogenase (Cell Signaling; s31866), acetyl-coenzyme A acetyltransferase 1 (Cell Signaling; s44276), carnitine palmitoyltransferase 1a (Cell Signaling; s97361), glutamate dehydrogenase (Cell Signaling; s12793), vinculin (Cell Signaling; s13901), βactin (Cell Signaling; s4970). After washing, membranes were incubated 1h at room temperature with HRP-linked goat anti-rabbit antibody (Cell Signaling; s7074). Bands were detected using enhanced chemiluminescence (ECL) and their densities quantified using ImageLab software (Bio-rad). Each band was delimited manually, and densities were normalized by dividing by the density of controls [38] (link).
Carnitine palmitoyltransferase 1a
Manufactured by Cell Signaling Technology
Carnitine palmitoyltransferase 1a is an enzyme that catalyzes the transfer of a fatty acyl group from coenzyme A to carnitine, the first step in the mitochondrial beta-oxidation of long-chain fatty acids.
Automatically generated - may contain errors
Lab products found in correlation
Glutamate dehydrogenase, by Cell Signaling Technology (2 mentions)
Vinculin, by Cell Signaling Technology (2 mentions)
P pyruvate dehydrogenase, by Cell Signaling Technology (2 mentions)
Acetyl coenzyme a acetyltransferase 1, by Cell Signaling Technology (2 mentions)
Hrp linked goat anti rabbit antibody, by Cell Signaling Technology (2 mentions)
P mtor, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Image lab software, by Bio-Rad (2 mentions)
Trans blot turbo transfer system, by Bio-Rad (2 mentions)
2 protocols using carnitine palmitoyltransferase 1a
1
Striatal Protein Expression Analysis
2
Striatal Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Striatum samples were homogenized by sonication in SDS buffer (10%). After determining the dynamic range of detection for each antibody, 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly, membranes were incubated overnight at 4°C with primary antibodies of interest anti-: TH (Cell Signaling; s58844), mTOR (Cell Signaling; s2983), p-mTOR (Cell Signaling; s5536), iNOS (Cell Signaling; s13120), MPC1 (Cell Signaling; s14462), pyruvate dehydrogenase (Cell Signaling; s3205), p-pyruvate dehydrogenase (Cell Signaling; s31866), acetyl-coenzyme A acetyltransferase 1 (Cell Signaling; s44276), carnitine palmitoyltransferase 1a (Cell Signaling; s97361), glutamate dehydrogenase (Cell Signaling; s12793), vinculin (Cell Signaling; s13901), βactin (Cell Signaling; s4970). After washing, membranes were incubated 1h at room temperature with HRP-linked goat anti-rabbit antibody (Cell Signaling; s7074). Bands were detected using enhanced chemiluminescence (ECL) and their densities quantified using ImageLab software (Bio-rad). Each band was delimited manually, and densities were normalized by dividing by the density of controls [38] (link).
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