The insertion mutants SALK_034653 for
vdac1 and SALK_127899.41.10.x for
vdac3 [5 (
link)] were from the Columbia ecotype. Both lines were kindly provided by Dr. Filleur (I2BC, Gif/Yvette, France).
vdac1_OEV3 corresponds to a
vdac1 mutant transformed with the VDAC3 genomic sequence (from its promoter to the end of its short 3′ UTR, and with a HA tag at the beginning of the coding sequence (CDS) [7 (
link)].
Arabidopsis cell cultures were from the Landsberg ecotype [8 (
link)].
Plants were grown in long-day conditions (16 h day at 21 °C/8 h night at 18 °C cycles, LED tubes Philips
1500 mm SO 20W 840 T8, photon flux density of 120 mmol/s/m
2 at the plant level). Seedling cultures and cell cultures were grown at 23 °C with constant light. Seedlings were grown in
Murashige and Skoog MS231 medium (Duchefa Biochemie, Haarlem, The Netherlands) (8 days in hydroponic cultures or 10 days on plates for O
2 consumption experiments). Cell cultures were grown in Murashige and Skoog MS256 medium.
Stress conditions were performed on 8-day-old cell cultures. Cold and heat shocks were for 3 h at 4 °C or 37 °C, respectively. NaCl (150 mM) and H
2O
2 (20 mM) stresses and phosphate starvation were for 24 h at 23 °C. For phosphate starvation studies, media used were MSP01 (with phosphate) and MSP11 (without phosphate) (Caisson Labs, Smithfield, UT, USA).
Hemono M., Ubrig É., Azeredo K., Salinas-Giegé T., Drouard L, & Duchêne A.M. (2020). Arabidopsis Voltage-Dependent Anion Channels (VDACs): Overlapping and Specific Functions in Mitochondria. Cells, 9(4), 1023.