Cells were fixed in a 0.1% (v/v) gluteraldehyde and 4% (v/v) paraformaldehyde solution and embedded in the low viscosity embedding Spurr media. Ultrathin sections were cut, placed on nickel grids and incubated in sodium metaperiodate. Samples were blocked in 1% (v/v) BSA for 5 min, incubated 60 min in a goat anti-human gal-7 polyclonal antibody (1:150) and 60 min in a rabbit anti-goat 10 nm gold-conjugated secondary antibody (1:20, Electron Microscopy Sciences, Hatfield, PA). Each section were counterstained with uranyle acetate and lead citrate and visualized using a Hitachi 7100 transmission electron microscope.
Rabbit anti goat 10 nm gold conjugated secondary antibody
Manufactured by Electron Microscopy Sciences
Sourced in United States
The Rabbit anti-goat 10 nm gold-conjugated secondary antibody is a laboratory reagent designed for use in various immunoassay techniques. It is composed of a rabbit-derived antibody that specifically binds to goat primary antibodies, with 10 nanometer gold particles conjugated to it. This secondary antibody can be utilized to detect and visualize the location of target antigens in a sample when used in conjunction with a goat primary antibody.
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2 protocols using rabbit anti goat 10 nm gold conjugated secondary antibody
1
Immunogold Labeling of Galectin-7
2
Immunogold Labeling of Galectin-7
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Cells were fixed in a 0.1% (v/v) glutaraldehyde and 4% (w/v) paraformaldehyde solution and embedded in Spurr’s resin. Ultrathin sections were placed on nickel grids and incubated in sodium metaperiodate. Samples were then blocked in 1% PBA for 5 min and incubated for 60 min with a goat anti-human gal-7 polyclonal antibody (1:150) followed by incubation with a rabbit anti-goat 10-nm gold-conjugated secondary antibody (1:20, Electron Microscopy Sciences, Hatfield, PA, USA). The samples were counterstained with uranyl acetate and lead citrate before visualization under a Hitachi H-7100 transmission electron microscope.
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