Western immunoblotting was performed as reported.21 (link) Primary antibodies used were: RIOK3 (H00008780-M01, Abnova), HIF1α (610959, BD Transduction Laboratories), HIF2α (NB100-122; Novus), CA9 (clone M75, gift of J. Pastorek, Bratislava), TPM3 (ab113692, Abcam), TPM3 (HPA009066, Sigma-Aldrich), TMOD3 (HPA001849, Sigma-Aldrich) and Actin-HRP (A3854; Sigma-Aldrich). Band densitometry was performed using the Analyze Gels tool in ImageJ (http://imagej.nih.gov/ij , version 1.47q).
Ab113692
Manufactured by Abcam
Sourced in United States
Ab113692 is a lab equipment product. It is a mono-specific antibody targeting a specific antigen. The core function of this product is to facilitate the detection and analysis of the target antigen in research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Hif 1α, by BD (2 mentions)
Hif 2α nb100 122, by Novus Biologicals (2 mentions)
Actin hrp, by Merck Group (2 mentions)
Hpa001849, by Merck Group (2 mentions)
Riok3, by Abnova (2 mentions)
Ca9 clone m75, by Abcam (2 mentions)
Tmod3, by Merck Group (2 mentions)
A21057, by Thermo Fisher Scientific (1 mentions)
A21109, by Thermo Fisher Scientific (1 mentions)
Zb 2305, by ZSGB-BIO (1 mentions)
F2168, by Merck Group (1 mentions)
Ac026, by ABclonal (1 mentions)
Ab11316, by Abcam (1 mentions)
Be2011, by EASYBIO (1 mentions)
Ac008, by ABclonal (1 mentions)
Alexa fluor 680 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Zf 0311, by ZSGB-BIO (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Hyblot cl autoradiography film, by Thomas Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
4 protocols using ab113692
1
Western Blot Analysis of Hypoxia Markers
2
Antibody Staining Protocol for Cell Imaging
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Mouse antibody to α‐Tubulin‐FITC (1:400, F2168) was purchased from Sigma (USA). Mouse antibodies to γ‐Tubulin (1:200, ab11316) and TPM3 (1:100, ab113692) were purchased from Abcam (USA). Mouse antibody to FLAG (1:2000, M20008L) was purchased from Abmart (Shanghai, China). Rabbit antibody to MYC (1:1000, BE2011) was purchased from EASYBIO (Beijing, China). Rabbit antibodies to β‐Actin (1:2000, AC026) and β‐Tubulin (1:1000, AC008) were purchased from ABclonal (Wuhan, China). Rabbit antibodies to RNF20 (1: 1000, 21625‐1‐AP) and TPM3 (1:1000, 10737‐1‐AP) were purchased from Proteintech Group (USA). Rabbit antibodies to H2Bub (1: 1000, 5546s), H2B (1: 1000, 12364S) and H3 (1: 1000, 4499S) were purchased from Cell Signaling Technology (USA). Mouse antibody to HEC1 (1: 100, sc‐515510) was purchased from Santa Cruz Biotechnology (USA). Rabbit antibodies to RBBP7 (1:100, bs8620) and UBASH3B (1:100, bs8741) were purchased from Bioworld (Beijing, China). Human antibody to ACA (1: 200, 15–234) was purchased from Antibodiesinc (USA). Goat anti‐rabbit FITC (1:200, ZF‐0311) and goat anti‐mouse TRITC (1:200, ZB‐2305) were purchased from ZSGB‐BIO (Beijing, China). Alexa Fluor 680‐conjugated goat anti‐mouse (1:10000, A21057) and Alexa Fluor 800‐conjugated goat anti‐rabbit (1:10000, A21109) were purchased from Invitrogen (USA).
3
Western Blot Analysis of Hypoxia Markers
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Western immunoblotting was performed as reported.21 (link) Primary antibodies used were: RIOK3 (H00008780-M01, Abnova), HIF1α (610959, BD Transduction Laboratories), HIF2α (NB100-122; Novus), CA9 (clone M75, gift of J. Pastorek, Bratislava), TPM3 (ab113692, Abcam), TPM3 (HPA009066, Sigma-Aldrich), TMOD3 (HPA001849, Sigma-Aldrich) and Actin-HRP (A3854; Sigma-Aldrich). Band densitometry was performed using the Analyze Gels tool in ImageJ (http://imagej.nih.gov/ij , version 1.47q).
4
Western Blot Analysis of Tropomyosin Proteins
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Cell pellets were resuspended in Laemmli buffer, sonicated for 5 min, and boiled for 5 min at 95 °C. Lysates were centrifuged at 10,000 rpm for 5 min at room temperature, and supernatants were used for analysis. Lysate volumes were normalized to cell counts. Samples were run on 4–12% NuPAGE Bis-Tris gels (Invitrogen) and transferred onto nitrocellulose membranes (0.45um pore size, Invitrogen) at 350 mA for 90 min. Following blocking in 5% milk for 1 h, membranes were incubated with primary antibodies overnight at 4 °C. After washing thrice in TBST, membranes were incubated with secondary horseradish peroxidase-conjugate antibodies for 1 h at room temperature, washed in TBST thrice, and developed using ECL western blotting substrate (Pierce) and HyBlot CL autoradiography film (Denville Scientific). The following antibodies were used for western blotting: Rabbit anti-TPM1 (D12H4, #3910, Cell Signaling Technologies), Mouse anti-TPM1/TPM2 (15D12.2, MAB2254, Millipore Sigma), Mouse anti-TPM3 (3D5AH3AB4, ab113692, Abcam), Rabbit anti-TPM4 (AB5449, Millipore Sigma), and Mouse anti-β Actin (A1978, Sigma). Western blot band quantitation was performed using FIJI [79 (link)] (https://fiji.sc/ ).
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