Human PC cell lines AsPC‐1 and PaCa‐2 and human pancreatic duct epithelial cell line HPDE6‐C7 were obtained from ATCC (Rockville, MD, USA). The cells were cultured in RPMI‐1640 or DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS. The DLEU2 expression plasmid (pcDNA3.1‐DLEU2) containing DLEU2 (variant uc001vdy.3), and the SMAD2 expression plasmid pcDNA3.1‐SMAD2 were constructed by GenePharma (Shanghai, China). In addition, siRNAs targeting DLEU2, siRNAs targeting SMAD2, control siRNA, miR‐455 mimic, miR‐15a mimic, miR‐16 mimic, control mimic, miR‐455 inhibitor, and control inhibitor were prepared by RiboBio (Guangzhou, China). Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) was used for cell transfection following the manufacturer's instructions.
Mir 15a mimic
Manufactured by RiboBio
Sourced in China
The MiR-15a mimic is a synthetic RNA molecule that mimics the function of the naturally occurring microRNA (miRNA) miR-15a. miRNAs are small, non-coding RNA molecules that play a crucial role in gene expression regulation. The MiR-15a mimic can be used in research applications to study the biological functions and molecular mechanisms associated with miR-15a.
Automatically generated - may contain errors
Lab products found in correlation
Inhibitor control, by RiboBio (1 mentions)
Mir 16 mimic, by RiboBio (1 mentions)
Hpde6 c7, by American Type Culture Collection (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Sirnas, by RiboBio (1 mentions)
Control sirna, by RiboBio (1 mentions)
Mimic control, by RiboBio (1 mentions)
Supersignal west pico chemiluminescence kit, by Thermo Fisher Scientific (1 mentions)
Quant sybr green pcr kit, by Tiangen Biotech (1 mentions)
Mir 194 mimic, by RiboBio (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Mir 128 mimic, by RiboBio (1 mentions)
Lipfectamine 2000, by Thermo Fisher Scientific (1 mentions)
Diaminobenzidine dab, by Agilent Technologies (1 mentions)
Metformin, by Merck Group (1 mentions)
Trizol reagent, by Tiangen Biotech (1 mentions)
Fastquant rt kit, by Tiangen Biotech (1 mentions)
2 protocols using mir 15a mimic
1
DLEU2 and SMAD2 Regulation in Pancreatic Cells
2
Molecular Mechanisms of AMPK Regulation
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Antibodies against phosphorylated α (p-AMPKα Thr172) and non-phosphorylated AMPKα were purchased from Cell Signaling Technology (Danvers, MA, USA); Antibody against Bmi-1 was from Abcam (Cambridge, MA, USA); Antibodies against LKB1 and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-LITAF antibody was from Abnova Corporation (Taiwan, China). Diaminobenzidine (DAB) was from Dako (Carpinteria, CA, USA). Enzyme Antibody Conjugates were from ZSGB-BIO (Beijing, China). Metformin and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were from Sigma-Aldrich (St Louise, MO, USA). Trizol reagent, FastQuant RT Kit, and Quant SYBR Green PCR kit were from TIANGEN Biotech (Beijing, China). Lipfectamine 2000 was from Life Technologies (Grand Island, NY). miR-15a mimic, miR-128 mimic, miR-192 mimic, miR-194 mimic, and negative control mimic were purchased from Ribobio (Guangzhou, China). SuperSignal West Pico Chemiluminescence kit was from Thermo-Fisher scientific (Waltham, MA, USA).
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