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Dmire2 epifluorescence microscope

Manufactured by Leica
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The DMIRE2 is an epifluorescence microscope manufactured by Leica. It is designed to perform fluorescence imaging and analysis. The instrument provides essential functions for fluorescence microscopy, including excitation light sources, filter sets, and imaging capabilities.

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Lab products found in correlation

Fluorescence mounting medium, by Agilent Technologies (2 mentions) Volocity 4, by PerkinElmer (2 mentions) Back thinned electron multiplying charge coupled device camera, by PerkinElmer (2 mentions) Orca er camera, by Hamamatsu Photonics (1 mentions) Alexa488 coupled anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Copas biosort, by Union Biometrica (1 mentions) Retiga 1300, by Zeiss (1 mentions) Fluoroshield with dapi, by Merck Group (1 mentions) 8 well glass slides, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Goat anti rabbit igg cfl 555, by Santa Cruz Biotechnology (1 mentions) Volocity software, by PerkinElmer (1 mentions)

Spelling variants of this product

DMIRE2 (204 citations) Epifluorescence microscope (146 citations) DMIRE2 microscope (135 citations) DMIRE2 epifluorescence/DIC microscope (2 citations)

7 protocols using dmire2 epifluorescence microscope

1

Immunohistochemical Analysis of Embryonic Ephrin Signaling

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Wild-type embryos were fixed at stage 10.5, and sagittal cryosections were prepared as described [55] (link),[56] (link). Sections were stained with anti–phospho-EphB antibody and Alexa488-coupled anti-rabbit IgG (Invitrogen). Images were collected with a DMIRE2 epifluorescence microscope (Leica) equipped with a 20×/0.70IMM Corr CS oil immersion objective and an ORCA-ER camera (Hamamatsu Photonics), controlled with Metamorph software.
Rohani N., Parmeggiani A., Winklbauer R, & Fagotto F. (2014). Variable Combinations of Specific Ephrin Ligand/Eph Receptor Pairs Control Embryonic Tissue Separation. PLoS Biology, 12(9), e1001955.
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2

Yeast Transformant Immunofluorescence Microscopy

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Yeast transformants were cultivated in 2% w/v raffinose SC-Ura medium supplemented with 0.4% w/v galactose and 250 μM ALA to midlog phase and then fixed with 4% formaldehyde for 1 h at room temperature. Immunofluorescence microscopy was performed as described elsewhere (23 ). Images were taken using a DM IRE2 epifluorescence microscope (Leica, Wetzlar, Germany) connected to a Retiga 1300 cooled Mono 12-bit camera (Retiga, QImaging, Surry, BC, Canada).
Luck A.N., Yuan X., Voronin D., Slatko B.E., Hamza I, & Foster J.M. (2016). Heme acquisition in the parasitic filarial nematode Brugia malayi. The FASEB Journal, 30(10), 3501-3514.
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3

Worm Phenotypic Analysis by COPAS

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Worms were grown from the L1 larval stage to the early adult stage on RNAi plates. Worms for each condition were analyzed for time of flight (length) and extinction (optical density) using a COPAS BioSort (Union Biometrica, Holliston, MA) with gating parameters for mixed worm populations as in Chen et al (Chen et al., 2011 (link)). GFP, YFP, and ZnMP fluorescence in worms was imaged using a DMIRE2 epifluorescence microscope (Leica) connected to a Retiga 1300 cooled mono 12-bit camera or using a laser scanning confocal microscope (LSM710) (Zeiss).
Korolnek T., Zhang J., Beardsley S., Scheffer G.L, & Hamza I. (2014). Control of metazoan heme homeostasis by a conserved multidrug resistance protein. Cell metabolism, 19(6), 1008-1019.
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4

Immunofluorescence Staining of Interphase and Mitotic Cells

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For imaging of interphase and mitotic cells, U2OS cells were seeded on 8-well glass slides (Millipore) and either synchronized at the G2/M transition and released as described or grown asynchronously. Immunofluorescence staining was performed by first washing cells once with 1 × PBS and fixing with a 3.7% Paraformaldehyde (PFA) in PBS solution for 10 min at room temperature. Fixed cells were permeabilized for 10 min on ice using 0.5% Triton X-100 in 1 × PBS and then blocked for 30 min in PBS-T (0.1% Tween 20 in PBS) containing 1% BSA at room temperature. Slides were then incubated with primary antibody diluted in PBS-T for 1 h at room temperature followed by three 5 min washes in PBS. After washing, the slides were then incubated with secondary antibodies, AlexaFluor 488 goat anti-mouse (Life Technologies) and goat anti-rabbit IgG-CFL 555 (Santa Cruz), in PBS-T for 1 h and washed three times with 1 × PBS. Coverslips were mounted using Fluoroshield with DAPI (Sigma) and sealed with clear nail polish. Slides were then imaged on an SP8 confocal microscope (Leica, Wetzlar, Germany) or a DM IRE2 epi-fluorescence microscope (Leica) and images processed using the Fiji distribution of ImageJ (34 (link)).
Dilworth D., Gudavicius G., Xu X., Boyce A.K., O’Sullivan C., Serpa J.J., Bilenky M., Petrochenko E.V., Borchers C.H., Hirst M., Swayne L.A., Howard P, & Nelson C.J. (2018). The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stability. Nucleic Acids Research, 46(5), 2459-2478.
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5

Immunostaining of Intracellular Listeria

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Immunostaining was conducted as previously described [26 (link)]. In brief, after infections, cells were fixed using 2.5% paraformaldehyde for 10 min at 37°C. Extracellular L. monocytogenes were detected by immunostaining prior to permeabilization. Cells were then permeabilized and blocked using 0.2% saponin with 10% normal goat serum for 14–16 h at 4°C. All colocalization quantifications were done using a Leica DMIRE2 epifluorescence microscope equipped with a 100X oil objective, 1.4 numerical aperture. 100 intracellular bacteria were examined in each experiment. Images are single confocal z-slices taken using a Zeiss Axiovert confocal microscope and LSM 510 software. Volocity software (Improvision) was used to analyze images. Images were imported into Adobe Photoshop and assembled in Adobe Illustrator.
Cemma M., Lam G.Y., Stöckli M., Higgins D.E, & Brumell J.H. (2015). Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells. PLoS ONE, 10(5), e0125856.
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6

Immunostaining Protocol for Microscopy

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Immunostaining was conducted as previously described39 . Briefly, cells were permeabilized and blocked in PBS containing 0.2% saponin (Calbiochem) and 10% normal goat serum (SS-PBS) for 30 min. Subsequently cells were incubated for 1 h with primary antibodies in SS-PBS. Cells were washed three times with PBS and incubated with secondary AlexaFluor conjugated antibodies for 1 h. Cells were washed three times with PBS, mounted in fluorescence mounting medium (Dako), and analyzed using a Leica DMIRE2 epifluorescence microscope. Confocal pictures were taken using a Quorum spinning disk microscope (Leica DMIRE2 inverted fluorescence microscope equipped with a Hamamatsu back-thinned electron multiplying charge-coupled device camera, spinning disc head, and Volocity 4 software (Improvision). Confocal images were imported into Adobe Photoshop and assembled in Adobe Illustrator for labeling.
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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7

Immunostaining Protocol for Microscopy

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Immunostaining was conducted as previously described39 . Briefly, cells were permeabilized and blocked in PBS containing 0.2% saponin (Calbiochem) and 10% normal goat serum (SS-PBS) for 30 min. Subsequently cells were incubated for 1 h with primary antibodies in SS-PBS. Cells were washed three times with PBS and incubated with secondary AlexaFluor conjugated antibodies for 1 h. Cells were washed three times with PBS, mounted in fluorescence mounting medium (Dako), and analyzed using a Leica DMIRE2 epifluorescence microscope. Confocal pictures were taken using a Quorum spinning disk microscope (Leica DMIRE2 inverted fluorescence microscope equipped with a Hamamatsu back-thinned electron multiplying charge-coupled device camera, spinning disc head, and Volocity 4 software (Improvision). Confocal images were imported into Adobe Photoshop and assembled in Adobe Illustrator for labeling.
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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