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Yapgkf nh2

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YAPGKF-NH2 is a custom-synthesized peptide. It is a short chain of amino acids with the sequence tyrosine-alanine-proline-glycine-lysine-phenylalanine. This peptide is commonly used for research purposes in biochemistry and molecular biology laboratories.

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Lab products found in correlation

Par4 ap aypgkf nh2, by Peptide Institute (3 mentions) H e staining reagents, by Thermo Fisher Scientific (3 mentions) Fr180204, by Merck Group (2 mentions) N acetylcysteine amide, by Bio-Techne (2 mentions) Ethyl pyruvate, by Merck Group (1 mentions) Methylcellulose, by Merck Group (1 mentions) Sb225002, by Bio-Techne (1 mentions) Amd3100, by Bio-Techne (1 mentions)

4 protocols using yapgkf nh2

1

Peptide Treatments in Disease Model

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PAR4-AP (AYPGKF-NH2) and corresponding scrambled peptide (YAPGKF-NH2) were from Peptides International, Inc. (Louisville, KY, USA). Disulfide HMGB1 peptides were from HMGBiontech (Milano, Italy). N-Acetylcysteine amide was purchased from Tocris (Minneapolis, MN, USA). FR180204 was from Sigma-Aldrich. H&E staining reagents were from Fisher Scientific. The rest of the materials used were from Sigma-Aldrich or as described in the methods.
Ye S., Mahmood D.F., Ma F., Leng L., Bucala R, & Vera P.L. (2023). Urothelial Oxidative Stress and ERK Activation Mediate HMGB1-Induced Bladder Pain. Cells, 12(10), 1440.
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2

Peptide-induced Inflammation Modulation

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PAR4-AP (AYPGKF-NH2; to elicit BHA) and corresponding scrambled peptide (YAPGKF-NH2; as control) were from Peptides International, Inc. (Louisville, KY). N-Acetylcysteine amide was purchased from Tocris (Minneapolis, MN). FR180204, ethyl pyruvate and methyl cellulose were from Sigma-Aldrich. H&E staining reagents were from Fisher Scientific. Mouse HMGB1 enzyme-linked immunosorbent assay (ELISA, MBS2701751) kits were obtained from MyBioSource, Inc. (San Diego, CA). The rest of the materials used were from Sigma-Aldrich or as described in the methods.
Ye S., Ma F., Mahmood D.F., Meyer-Siegler K.L., Leng L., Bucala R, & Vera P.L. (2022). Bladder Oxidative Stress and HMGB1 Release Contribute to PAR4-Mediated Bladder Pain in Mice. Frontiers in Systems Neuroscience, 16, 882493.
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3

Evaluating anti-MIF and anti-CD74 Modulators

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PAR4-AP (AYPGKF-NH2)] and corresponding scrambled peptide (YAPGKF-NH2) as control were from Peptides International, Inc. (Louisville, KY). Anti-MIF monoclonal antibody, isotope control (mIgG1) and anti-CD74 monoclonal antibodies were provided by Dr. Richard Bucala from Yale University. Rat IgG2b monoclonal antibodies (isotype controls for anti-CD74) were from BD Biosciences (San Jose, CA). AMD3100 and SB225002 were purchased from Tocris (Minneapolis, MN). HE staining reagents were from Fisher Scientific. The rest of the materials used were from Sigma-Aldrich or as described in the methods.
Ye S., Ma F., Mahmood D.F., Meyer-Siegler K.L., Menard R.E., Hunt D.E., Leng L., Bucala R, & Vera P.L. (2021). Intravesical CD74 and CXCR4, macrophage migration inhibitory factor (MIF) receptors, mediate bladder pain. PLoS ONE, 16(8), e0255975.
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4

Intrathecal Anti-MIF mAb Alleviates Bladder Pain

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Female mice received repeated intravesical instillation of PAR4-AP as described above to set up persistent bladder pain. Repeated instillation with a scrambled peptide (YAPGKF-NH2; Peptides International, Louisville, KY, USA) served as a “no pain” control group [7 (link)].
On day 7, mice were divided into four groups (n = 9/group):

Group 1: Scrambled peptide + sham lumbosacral intrathecal injection (control; no pain group)

Group 2: PAR4-AP peptide + sham lumbosacral intrathecal injection (pain group + sham treatment)

Group 3: PAR4-AP peptide + lumbosacral intrathecal anti-MIF mAb injection (pain group + treatment)

Group 4: PAR4-AP peptide + lumbosacral intrathecal mouse IgG1 injection (pain group + treatment-control)

At the peak of the analgesic effect (2 h, as determined in Experiment 1A), mice were re-anesthetized with isofluorane, the lumbosacral spinal segments were exposed by laminectomy, and the spinal cord was removed, snap-frozen, and stored in liquid nitrogen until analysis.
Ye S., Agalave N.M., Ma F., Mahmood D.F., Al-Grety A., Khoonsari P.E., Leng L., Svensson C.I., Bucala R., Kultima K, & Vera P.L. (2024). MIF-Modulated Spinal Proteins Associated with Persistent Bladder Pain: A Proteomics Study. International Journal of Molecular Sciences, 25(8), 4484.
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