Leukotriene c4 ltc4

Alamethicin, β-estradiol, dipyridamole (DIPY), D-saccharic-1, 4-lactone, magnesium chloride (MgCl₂), MK-571, Ko143, leukotriene C4 (LTC4) and uridine diphosphate glucuronic acid (UDPGA) were all obtained from Sigma-Aldrich (St Louis, MO). Apigenin, chrysin, cycloicaritin, genistein, and wushanicaritin with purity over 98% was purchased from Aladdin Reagents (Shanghai, China). Apigenin-7-O-glucuronide, chrysin-7-O-glucuronide, genistein-7-O-glucuronide, and β-estradiol-3-O-glucuronide was purchased from Toronto Research Chemicals (North York, ON, Canada). Wushanicaritin-3-O-glucuronide was prepared in our laboratory as described previously [23 (link)]. 293T cells, HeLa cells, pLVX-mCMV-ZsGreen-PGK-Puro vector [9371 base pairs (bp)] and pLVX-shRNA2-Neo vector (9070 bp) were all provided from BioWit Technologies (Shenzhen, China). The pGEM-T plasmid which carried the UGT1A1 cDNA clone was purchased from Sino Biological Inc. (Beijing, China), while recombinant human UGT1A1 was purchased from Corning Biosciences (New York, USA). The anti-BCRP, anti-GAPDH, anti-MRP1, anti-MRP2, anti-MRP3, anti-MRP4 and anti-UGT1A1 antibodies were all obtained from OriGene Technologies (Rockville, MD). All other chemicals and reagents were the highest grade commercially available.

The pLVX-mCMV-ZsGreen-PGK-Puro vector [9371 base pairs (bp)], and a pLVX-shRNA2-Neo vector (9070 bp) were obtained from BioWit Technologies (Shenzhen, China). Expressed human UGT1A1 was purchased from Corning Biosciences (New York). Alamethicin, β-estradiol, β-estradiol-3-O-glucuronide, dipyridamole (DIPY), D-saccharic-1,4-lactone, magnesium chloride (MgCl₂), MK-571, Ko143, leukotriene C4 (LTC4), and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO). UGT1A1, GADPH, BCRP, MRP1, MRP3, and MRP4 antibodies were all purchased from OriGene Technologies (Rockville, MD). Bisdemethoxycurcumin was provided from Guangzhou Fans Biotechnology Co., Ltd (Guangzhou, China). All other chemicals and reagents were of guaranteed reagent or the highest grade commercially available.