13c17 afb1

Manufactured by Romer Labs

Sourced in Austria

The 13C17-AFB1 is a laboratory instrument designed for the detection and quantification of aflatoxin B1 (AFB1) in various sample matrices. It utilizes a carbon-13 (13C) labeled internal standard to provide accurate and precise measurements of AFB1 concentrations. The core function of this product is to facilitate the analysis of AFB1 levels in food, feed, and other relevant samples.

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Lab products found in correlation

13c20 ota, by Romer Labs (2 mentions) Milli q system, by Merck Group (2 mentions) Formic acid, by Biosolve (2 mentions) Ammonium formate, by Thermo Fisher Scientific (1 mentions) 3 acdon, by Romer Labs (1 mentions) Hplc grade meoh, by Thermo Fisher Scientific (1 mentions) 15 acdon, by Romer Labs (1 mentions) 13c34 fb1, by Romer Labs (1 mentions) 13c15 don, by Merck Group (1 mentions) Glacial acetic acid, by Biosolve (1 mentions) 13c15 don, by Romer Labs (1 mentions) Oasis prime hlb 96 well plate, by Waters Corporation (1 mentions) Formic acid, by Avantor (1 mentions)

5 protocols using 13c17 afb1

Mycotoxin Analysis Protocol

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HPLC grade MeOH, ACN, ammonium formate, FA, and AA were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Deionized water was prepared using a Milli-Q system (Millipore Corporation, Bedford, MA, USA). The MycoSpin^TM 400 SPE cartridge was purchased from Romer Labs (Tulln, Austria). Certified standard solutions of AFB₁ (25 μg mL⁻¹), AFB₂ (25 μg mL⁻¹), AFM₁ (10 μg mL⁻¹), AFM₂ (10 μg mL⁻¹), AFG₁ (25 μg mL⁻¹), AFG₂ (25 μg mL⁻¹), 15-AcDON (100 μg mL⁻¹), 3-AcDON (100 μg mL⁻¹), DAS (, 100 μg mL⁻¹), HT-2 (100 μg mL⁻¹), T-2 (100 μg mL⁻¹), DOM-1 (50 μg mL⁻¹), DON (50 μg mL⁻¹), NEO (100 μg mL⁻¹), ZEN (25 μg mL⁻¹), ST (50 μg mL⁻¹), OTA (10 μg mL⁻¹), OTB (10 μg mL⁻¹), OTC (10 μg mL⁻¹), FB₁ (50 μg mL⁻¹), FB₂ (FB₂, 50 μg mL⁻¹),FB₃ (FB₃, 50 μg mL⁻¹), [¹³C₁₇]-AFB₁ (0.5 μg mL⁻¹), [¹³C₂₀]-OTA (10 μg mL⁻¹), and [¹³C₃₄]-FB₁ (25 μg mL⁻¹) were acquired from Romer Labs (Butzbach, Germany) with purities above 98%.

Liang H., Hou Q., Zhou Y., Zhang L., Yang M, & Zhao X. (2022). Centrifugation-Assisted Solid-Phase Extraction Coupled with UPLC-MS/MS for the Determination of Mycotoxins in ARECAE Semen and Its Processed Products. Toxins, 14(11), 742.

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Aflatoxin B1 Quantification in Smokeless Tobacco

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Reverse osmosis deionized water, HPLC grade solvents, and high-purity reagents were used throughout. Stock solutions of AFB₁ (2.0 ng/μL) as well as the internal standard ¹³C₁₇-AFB₁ (0.5 ng/μL) in acetonitrile were purchased (Romer Labs, Union, MO) and stored at -20 °C, from which calibrators were made as needed. Internal standard (100 μL) was diluted into 1900 μL acetonitrile to generate a solution of 12.5 pg/μL. 2S3 Kentucky Reference Moist Snuff (University of Kentucky, College of Agriculture, Lexington, KY) was used as a matrix for blanks, calibrators and quality control (QC) samples. A convenience sample of 32 commercially available smokeless tobacco products was acquired by purchasing products at the retail point-of-sale in the Washington, DC and Atlanta, GA metropolitan areas in 2013. The convenience sample consisted of 12 loose moist snuffs, 4 pouched moist snuffs, 6 dry snuffs, 7 chewing tobaccos, and 3 snus. A total of 17 product brands produced by 8 manufacturers were sampled. Tobacco products purchased in the Washington, DC area were shipped to CDC at room temperature and arrived within 48 h following shipment. Products were inventoried and then stored at -70 °C until further use.

Zitomer N., Rybak M.E., Li Z., Walters M.J, & Holman M.R. (2015). Determination of Aflatoxin B1 in Smokeless Tobacco Products by use of UHPLC-MS/MS. Journal of agricultural and food chemistry, 63(41), 9131-9138.

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Mycotoxins Standards Preparation

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AFB1, AFB2, AFM1 and AFG2 were purchased from Sigma-Aldrich (Germany). OTA, OTB, FB1, ¹³C₁₇-AFB1 and ¹³C₂₀-OTA were purchased from Romer Labs (Austria). ZEN, a-ZEL, b-ZEL, DON, 3-AcDON, 15-AcDON, NEO, HT-2, CIT, STE, T-2, ROC, MPA, CPA, Fus-X, ¹³C₂₂-HT-2, ¹³C₁₅-DON and ¹³C₂₄-T-2 were purchased from Chiron (Trondheim, Norway).
Standard stock solutions were prepared in acetonitrile and stored at −20 °C in amber glass vials. The mixture of the working solution was prepared by diluting each stock solution in acetonitrile. A combined multi-internal standard (ISTD) working solution contained ¹³C₁₇-AFB1 (2 ng/mL), ¹³C₂₀-OTA (20 ng/mL), ¹³C₂₂-HT-2 (30 ng/mL), ¹³C₁₅-DON (50 ng/mL) and ¹³C₂₄-T-2 (10 ng/mL).
All other chemicals and standards were obtained from Sigma-Aldrich (Germany) unless specified otherwise.

Al-Jaal B., Latiff A., Salama S., Hussain H.M., Al-Thani N.A., Al-Naimi N., Al-Qasmi N., Horvatovich P, & Jaganjac M. (2021). Analysis of Multiple Mycotoxins in the Qatari Population and Their Relation to Markers of Oxidative Stress. Toxins, 13(4), 267.

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Analytical Standards for Mycotoxins Quantification

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The analytical standards of DON, OTA, and AFB₁ were obtained from Fermentek (Jerusalem, Israel). The IS, ¹³C₁₅-DON, ¹³C₂₀-OTA, and ¹³C₁₇-AFB₁ were supplied by Biopure (Tulln, Austria). All standards were stored according to the recommendations of the supplier. Water, methanol (MeOH), ACN, glacial acetic acid, and formic acid for the mobile phases were of LC-MS grade and were obtained from Biosolve (Valkenswaard, The Netherlands).

Gallissot M., Rodriguez M.A., Devreese M., Van herteryck I., Molist F, & Santos R.R. (2024). An Algoclay-Based Decontaminant Decreases Exposure to Aflatoxin B1, Ochratoxin A, and Deoxynivalenol in a Toxicokinetic Model, as well as Supports Intestinal Morphology, and Decreases Liver Oxidative Stress in Broiler Chickens Fed a Diet Naturally Contaminated with Deoxynivalenol. Toxins, 16(5), 207.

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Quantitative Analysis of Aflatoxins in Food Samples

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The standards of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 were obtained from Fermentek Ltd. (Jerusalem, Israel) and had a certified purity of ≥ 98%. The internal standards (IS), ¹³C₁₇-AFB1, ¹³C₁₇-AFM1, and ¹³C₁₇-AFG1, were purchased as 0.5 µg mL⁻¹ solutions in ACN from Biopure (Tulln, Austria). All analytical standards were stored at the temperature suggested by the manufacturer.
Methanol (MeOH), ACN, and formic acid (FA) were of ULC-MS grade and were obtained from Biosolve (Valkenswaard, The Netherlands). ULC-MS grade water was taken from a Milli-Q system (Merck, Overijse, Belgium). All other solvents and reagents had an analytical grade (formic acid, magnesium sulfate (MgSO₄), sodium chloride (NaCl)) and were obtained from VWR (Leuven, Belgium).
Oasis^® Ostro protein precipitation & phospholipid removal 96-well plates (25 mg), Oasis^® PRiME HLB 96-well plates (30 mg), 2 mL square 96-well collection plates and 96-well cap-mats with square plugs (silicone/PTFE treated, pre-slit) were purchased from Waters (Antwerp, Belgium).

De Baere S., Ochieng P.E., Kemboi D.C., Scippo M.L., Okoth S., Lindahl J.F., Gathumbi J.K., Antonissen G, & Croubels S. (2023). Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS. Toxins, 15(1), 37.

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