Human brain vascular pericytes (HBVPs) were purchased from ScienCell (#1200) and maintained in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin at 37°C in 5% CO2‐humidified incubator. After reaching 80%‐90% confluence, the cells were passaged with trypsin (0.25%)‐EDTA (0.02%) in PBS at a split ratio of 1:5. The media were changed every 2 days.29 When reaching 60%‐70% confluence, the HBVPs were transfected with 10 μM Senp1 siRNA using Lipofectamine® RNAiMAX Reagent (13778, Invitrogen) for 48 h as described in the manual guide. Then, the cells were cultured with glucose‐free Hanks' Balanced Salt Solution (HBSS: 116 mM NaCl, 5.4 mM KCl, 0.8 mM MgSO4, 1.0 mM NaH2PO4, 1.8 mM CaCl2, and 26 mM NaHCO3, pH 7.3) for another 6 h. Thereafter, the cells were captured or used for Western blotting assay and immunofluorescence assay.
Hbvps
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HBVPs are a series of high-performance vacuum pumps designed for a variety of laboratory applications. They provide reliable and efficient vacuum generation to meet the needs of various laboratory processes. The core function of the HBVPs is to generate and maintain the desired level of vacuum within the specified operating parameters.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Hbvps, by ScienCell (1 mentions)
1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hbmec, by Thermo Fisher Scientific (1 mentions)
Hbmec, by ScienCell (1 mentions)
3 protocols using hbvps
1
Human Brain Vascular Pericyte Modeling
2
Cultivation of Human Brain Cells
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Human brain microvascular endothelial cell (HBMEC) and human brain vascular pericyte (HBVP) were purchased from Sciencell Research Laboratories (Carlsbad, CA, USA). The HBMECs were maintained in 1640 medium (Invitrogen) and HBVPs were cultured in Dulbecco's modified Eagle medium (DMEM, Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), penicillin (100 U/mL) (Invitrogen), and streptomycin (100 U/mL) (Invitrogen). The cultures were maintained at 37 oC in a 95% humidified atmosphere with 5% CO2.
3
Cytotoxicity of Cisplatin and Curcumin on HUVECs and HBVPs
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Human umbilical vein endothelial cells (HUVECs; Cat. No. #C-015-5C, Invitrogen, Waltham, MA, USA) and human brain vascular pericytes (HBVPs; Cat. No. 1200, ScienCell, Carlsbad, CA, USA) were grown according to manufacturers' protocols. Each experiment was performed in triplicate and repeated three times. HUVECs and HBVPs were divided into three groups: control, cisplatin, and cisplatin + curcumin. In the cisplatin and cisplatin + curcumin groups, HUVECs and HBVPs were incubated with 3 µg/mL and 1.5 µg/ /mL cisplatin (Cat. No. 1C 257/51, Korea United Pharm, Seoul, South Korea) for 24 hours, respectively. For curcumin treatment, the cells were co-incubated with 1 µg/mL curcumin (Cat. No. 81025, Cayman Chemical, Ann Arbor, MI, USA) for 24 hours. These doses were chosen as the lowest concentrations to induce cytotoxicity in the HUVEC and HBVP in the pilot studies.
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