1 ethyl 3

Manufactured by Merck Group

Sourced in Germany, India

1-ethyl-3 is a laboratory reagent commonly used in chemical synthesis and analysis. It serves as a key component in various organic reactions and experimental procedures. The core function of 1-ethyl-3 is to facilitate specific chemical transformations and analytical techniques, though its precise intended use may vary depending on the research or application context.

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Lab products found in correlation

Close spelling variants of this product

1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide 1-ethyl-3 (3-dimethylaminopropyl) carbodimide 1-ethyl-3-methylimidazolium tetrafluoroborate 1-ethyl-3-methylimidazolium trifluoromethanesulfonate (EMIM Triflate), 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride 1-ethyl-3-(3-(dimethylamino) -propyl)carbodimide (EDC), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) Bis‐2‐(5‐phenylacetamido‐1,3,4‐thiadiazol‐2‐yl) ethyl sulfide 1-ethyl-3-methylimidazolium acetate

6 protocols using 1 ethyl 3

Letrozole Drug Encapsulation Protocol

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Letrozole was donated by Rotex Pharmaceutical Industry, Islamabad, Pakistan. Sodium tripolyphosphate protamine sulphate, N-hydroxysuccinimide, and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCL) were purchased from Sigma.

Khan M.T., Uddin Z., Javed M.A., Shah N., Bashir H., Shaikh A.J., Rajoka M.S., Amirzada M.I, & Asad M.H. (2022). PEGylated Protamine Letrozole Nanoparticles: A Promising Strategy to Combat Human Breast Cancer via MCF-7 Cell Lines. BioMed Research International, 2022, 4438518.

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Biotinylation Kit for Protein Labeling

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The biotinylation kit, supplied by Sigma Immunochemicals (St. Louis, MO, USA), was composed of biotinylation Reagent (BAC-SulfoNHS, namely biotinamido hexanoic acid 3-sulfo-N-hydroxysuccinide ester), 5 M sodium chloride solution, micro-spin column (2 mL; in practice, a small empty cylindrical vessel prepackaged with Sephadex G-50), 0.1 M sodium phosphate buffer pH 7.2, 0.01 M phosphate buffer saline (PBS) pH 7.4 (reconstituted with 1 L of deionized water to give 0.01 M phosphate buffer, 0.138 M NaCl, 2.7 mM KCl, pH 7.4); and ExtrAvidin^® peroxidase (containing 0.2 mL of ExtrAvidin Peroxidase conjugate at 2.0 mg·mL⁻¹, supplied with 0.01% thimerosal). Dialysis membrane (art. D-9777), 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide, albumin (from bovine serum) (BSA) and TRIS (hydroxymethyl-aminomethane) and TWEEN^® 20 provided by Sigma Aldrich.

Tomassetti M., Martini E., Campanella L., Favero G., Sanzó G, & Mazzei F. (2015). A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices. Sensors (Basel, Switzerland), 15(5), 10255-10270.

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Aptamer-Hyaluronic Acid Conjugate Synthesis

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5TR1 aptamer (5TR1) (5′–COOH–GAAGTGAAAATGACAGAACACAACA-3′) was produced by MicroSynth (Switzerland). Hyaluronic acid (MW = 50,000 Da) (HA) was purchased from Creative PEGworks. The following materials were bought from Sigma-Aldrich in Munich, Germany: N-hydroxysulfosuccinimide (NHS), Adipic acid dihydrazide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), Epirubicin, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Merck in Darmstadt, Germany, supplied the other necessary materials.

Jamshidi Z., Dehghan R., Nejabat M., Abnous K., Taghdisi S.M, & Hadizadeh F. (2024). Dual-targeting CD44 and mucin by hyaluronic acid and 5TR1 aptamer for epirubicin delivery into cancer cells: Synthesis, characterization, in vitro and in vivo evaluation. Heliyon, 10(2), e24833.

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Immunomodulatory Hydrogel for Antigen Delivery

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HEMA, MAA, ethylene glycol dimethacrylate (EGDMA), potassium persulfate (KPS), poly(vinyl alcohol) (PVA) MW 31000–50000 hydrolyzed 98–99%, ovalbumin from chicken egg (OVA), Mannan from Saccharomyces cerevisiae, 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride (EDC), N-hydroxisuccinimide (NHS), MES sodium salt, Dulbecco’s Modified Eagle Medium (DMEM; high glucose without l-glutamine), phenol red free DMEM, penicillin, streptomycin, sterile 1× phosphate buffer saline (PBS), Hank’s buffered salt solution without (HBSS) calcium and magnesium, chloroacetic acid 99% A.C.S. grade, and Alexa Fluor594 wheat germ-agglutinin (WGA) were purchased from Sigma-Aldrich (St. Louis, MO). Sodium hydroxide (NaOH), hydrochloric acid (HCl), 100% ethanol, potassium chloride (KCl), glacial acetic acid, nitric acid, acetone, basic alumina, sulfo-N-hydroxisuccinimide (suflo-NHS), ethylendiamine dihydrochloride, and paraformaldehyde were obtained from Thermo Fisher Scientific Inc. (Fairlawn, NJ). CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) was purchased from VWR International (Radnor, PA), and Pro-Long with DAPI was acquired from Molecular Probes Invitrogen (Grand Island, NY).

Durán-Lobato M., Carrillo-Conde B., Khairandish Y, & Peppas N.A. (2014). Surface-Modified P(HEMA-co-MAA) Nanogel Carriers for Oral Vaccine Delivery: Design, Characterization, and In Vitro Targeting Evaluation. Biomacromolecules, 15(7), 2725-2734.

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Multifunctional Nanocarrier for Cancer Therapy

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Poly (ethyleneglycol) bis (3-aminopropyl ether) (PEG 1500); folic acid (FA); silver nitrate; poloxamer 407 (P-407); 1, 3-dicyclohexylcarbodiimide (DCC); 4-dimethylaminopyridine (DMAP); Dimethylsulfoxide (DMSO), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS), Dulbecco’s Minimal essential medium (DMEM), Fetal bovine serum (FBS), and MTT (3-(4, 5-Dimethylthiazol-2yl)-2, 5, diphenyltetrazoliumbromide) reagent were all purchased from Sigma-Aldrich, Bangalore, India. The National Centre for Cell Sciences in Pune, India, provided cancer cell lines such as HeLa (immortalized human cervical carcinoma) and Caco-2 (immortalized human epithelial colorectal adenocarcinoma) cells. Rhodamine B isothiocyanate, Toluidine Blue-O (TBO), Kaighn’s version of Ham’s F12 medium (F12K), Folate-free RPMI 1640 (FFRPMI), Fluorescence isothiocyanate (FITC), and EDTA/Trypsin were bought from BASF Corporation, Mumbai, India.

Al-Serwi R.H., Eladl M.A., El-Sherbiny M., Saleh M.A., Othman G., Alshahrani S.M., Alnefaie R., Jan A.M., Alnasser S.M., Albalawi A.E., Mohamed J.M, & Menaa F. (2023). Targeted Drug Administration onto Cancer Cells Using Hyaluronic Acid–Quercetin-Conjugated Silver Nanoparticles. Molecules, 28(10), 4146.

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Biopolymer Hydrogel Synthesis and Characterization

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Medium molecular weight alginate from brown algae (medium viscosity), Tris(Hydroxymethyl) aminomethane, Triton X-100, pepsin (from porcine gastric mucosa, 0.7 FIP-U/mg), 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, hydrochloride form), N-hydroxy-succinimide (NHS), tyramine hydrochloride, 2-(N-morpholino) ethanesulfonic acid (MES), horseradish peroxidase (HRP), dialysis bags (MWCO:12400), chondroitin sulfate C (shark cartilage extract), dimethylmethylene blue (DMMB), papain, and tetrazolium dye MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) were supplied by Sigma-Aldrich. Dimethyl sulfoxide (DMSO), sodium carbonate (Na 2 CO 3 ), glucono delta-lactone (GDL), calcium carbonate (CaCO 3 ), formic acid (98-100% v/v), methanol (99% v/v), and hydrochloric acid (HCl) were purchased from Merck (Darmstadt, Germany). Dulbecco's Modified Eagle's medium (DMEM, high glucose), fetal bovine serum (FBS), trypsin/EDTA (0.25% w/v), penicillin/streptomycin, trypan blue stain (0.4 % w/v), and phosphate buffer saline (PBS) were provided by Bio-Idea group. Hydrogen peroxide (H 2 O 2 ) was supplied by Dr. Mojallali Co. (Tehran Iran), and lithium bromide (LiBr) was purchased from Riedel-de Haën.

Shojarazavi N., Mashayekhan S., Pazooki H., Mohsenifard S, & Baniasadi H. (2021). Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering. Journal of biomaterials applications, 36(5).

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