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Anti helios af647

Manufactured by Thermo Fisher Scientific
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Anti-Helios-AF647 is a fluorescently-labeled antibody designed for use in flow cytometry applications. The antibody is conjugated to the Alexa Fluor 647 dye, which has excitation and emission wavelengths suitable for detection on common flow cytometry platforms. The core function of this product is to specifically bind and label target cells or cellular proteins for subsequent analysis by flow cytometry.

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Lab products found in correlation

Anti cd4 pacific blue, by BioLegend (2 mentions) Anti cd34 pe, by BioLegend (2 mentions) Anti cd1a percp cy5, by BioLegend (2 mentions) Anti cd1a pecy5, by BioLegend (2 mentions) Anti cd7 fitc, by BioLegend (2 mentions) Anti ikaros pe, by BD (2 mentions) Anti cd8 fitc, by BioLegend (1 mentions) Anti cd4 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd8 fitc, by BD (1 mentions) Anti cd8 bv785, by BioLegend (1 mentions) Anti cd34 pe cy7, by BioLegend (1 mentions) Anti cd3 apc cy7, by BioLegend (1 mentions) Anti mouse igg1κ pe, by BD (1 mentions) Anti ter119 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd44 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti eos pe, by Thermo Fisher Scientific (1 mentions) Anti cd24 pe, by Thermo Fisher Scientific (1 mentions) Anti cd4 pacific blue, by BD (1 mentions) Anti cd3 allophycocyanin apc cy7, by BioLegend (1 mentions) Anti cd44 pe cy7, by BioLegend (1 mentions)

2 protocols using anti helios af647

1

Multiparametric Immunophenotyping of Immune Cells

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The anti-mouse antibodies, anti-TER119-FITC, anti-CD4 FITC, anti-CD24-PE, anti-CD44-PE-Cy7, and anti-CD25-APCCy7, were purchased from eBioscience (San Diego, CA, USA). Anti-CD8-FITC, anti-CD8-AF647, and anti-CD4-Pacific Blue were purchased from BD Biosciences (San Jose, CA, USA). The anti-human antibodies, anti-CD1a-PerCP-Cy5.5, anti-CD1a-PECy5, anti-CD3-APCCy7, anti-CD4-Pacific Blue, anti-CD7-FITC, anti-CD8-BV785, Anti-CD8-FITC, anti-CD34-PE, anti-CD34-PECy7, and anti-CD38-AF700 were purchased from Biolegend (San Diego, CA). Anti-Aiolos-PE, anti-Eos-PE, and anti-Helios-AF647 were purchased from e-Bioscience. Anti-Mouse IgG1κ-PE and anti-Ikaros-PE were purchased from BD Biosciences. The anti-Armenian Hamster IgG-AF647 control was purchased from Biolegend.
Mitchell J.L., Seng A, & Yankee T.M. (2017). Expression and splicing of Ikaros family members in murine and human thymocytes. Molecular immunology, 87, 1-11.
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2

Multiparametric Flow Cytometry Panel

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The anti-human antibodies, anti-CD1a-PerCP-Cy5.5, anti-CD1a-PE-Cy5, anti-CD3− Allophycocyanin (APC)-Cy7, anti-CD4-Pacific Blue, anti-CD4-PE-CF594, anti-CD7-FITC, anti-CD7-APC, anti-CD8-Brilliant Violet (BV) 785, anti-CD28-Pacific Blue, anti-CD34-BV605, anti-CD34-PE, anti-CD38-Alexa Fluor (AF) 700, anti-CD44-PE-Cy7, and anti-TCRγδ-FITC were purchased from Biolegend (San Diego, CA). Anti-Helios-AF647 was purchased from e-Biosciences (San Diego, CA), and Mouse IgG1κ-PE control, anti-Ikaros-PE, and anti-Aiolos-PE were purchased from BD Biosciences (San Jose, CA). The Armenian Hamster IgG-AF647 control was purchased from Biolegend.
Mitchell J.L., Seng A, & Yankee T.M. (2016). Ikaros, Helios, and Aiolos protein levels increase in human thymocytes after β selection. Immunologic research, 64(2), 565-575.
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