Nontargeting control sirna

The siRNA sequences 5′-GUACAAAGAAGGUCACUUA-3′ and 5′-CUACUAUCUAGACUCGAAU-3′ targeted GTSE1 and siRNA 5′-GCAAGAUCCUGAAAGAGAU-3′ (Wandke et al., 2012 (link); Barisic et al., 2014 (link)), 5′-CAAUGUGCUCAGACGUAA-3′ (Stumpff et al., 2012 (link)), 5′-CCAAAUCAUUUGCCGAG-3′ (Stumpff et al., 2012 (link)), 5′-AGGCGUACAUUCUCCCUUA-3′ (Voets et al., 2015 (link)), 5′-GAAGAGGCCCACUGAAGUU-3′ (Voets et al., 2015 (link)), 5′-UGAAAGAGAUGUGCGAUGU3′ (Voets et al., 2015 (link)), and 5′-UGACUCGACUGCUUCAAGA-3′ (Voets et al., 2015 (link)) targeting Kif4A were synthesized by Sigma-Aldrich. The siRNA sequence 5′-GAGUAGAACUAGAAUGUGA-3′ targeting Hec1 was synthesized by QIAGEN. ON-TARGET plus SMARTpool siRNAs targeting GTSE1 (5′-ACACGUGGCUGUAGGAUCU-3′, 5′-GAACUGAACCAACAAGGGA-3′, 5′-UAAAUAAUCCGGUUCCCGA-3′, and 5′-GUACAAAGAAGCUCACUUA-3′) and Kif4A (5′-AGGCGUACAUUCUCCCUUA-3′, 5′-GAAGAGGCCCACUGAAGUU-3′, 5′-UGAAAGAGAUGUGCGAUGU-3′, and 5′-UGACUCGACUGCUUCAAGA-3′) were synthesized by GE Healthcare. Nontargeting control siRNA was synthesized by Bioneer.