Tnf α mbs824943

The serum IL-11 and other inflammatory factors levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 h after admission for all cases. Samples were centrifuged at 2000 g for 15 min, following with ELISA tested using commercially available kits (IL-11 EK0419 BOSTER, sensitivity < 10 pg/mL, detection range 31.2 pg/mL-2000 pg/mL, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, TNF-α MBS824943 MyBioSource). The serum cytokine levels were measured when patients were just hospitalized and 24 h, 48 h, 72 h, and 90 h after treatment.

The serum ACSL4, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-1β and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 hours after admission for all cases. Samples were centrifuged at 2000 g for 15 minutes, following with ELISA tested using commercially available kits (ACSL4 MBS9331516 MyBioSource, TNF-α MBS824943 MyBioSource, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, IL-1β MBS175901 MyBioSource).

The serum NLRP1, tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-1β and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 hours after admission for all cases. Samples were centrifuged at 2000 g for 15 minutes, followed with ELISA tested using commercially available kits (NLRP1 MBS924094 MyBioSource, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, TNF-α MBS824943 MyBioSource, IL-1β MBS175901 MyBioSource). In addition, routing whole blood test was performed using an automatic biochemical analyzer (Hitachi 7600, Hitachi Corporation, Japan) and the levels of interferon-γ and IL-4 were recorded. All these inflammatory factors were measured when the patients were just hospitalized.