Mica surface

Manufactured by Ted Pella

Sourced in United States

Mica surface is a type of natural mineral material commonly used as a substrate in various laboratory applications. It is a layered silicate mineral known for its unique physical properties, including transparency, electrical insulation, and thermal stability. The mica surface provides a smooth, flat, and inert platform for various experimental setups and sample preparations.

Automatically generated - may contain errors

Lab products found in correlation

Dimension fastscan afm, by Bruker (2 mentions) Nanoscope 4, by Digital Instruments (2 mentions) Metal puck, by Ted Pella (2 mentions) E scanner, by Bruker (2 mentions) Scanasyst fluid probe, by Bruker (2 mentions) Scanasyst, by Veeco (1 mentions) Nsc15, by MikroMasch (1 mentions) Nanoscope iiia controller, by Veeco (1 mentions) Ac160ts r3, by Olympus (1 mentions) Vo 200, by Memmert (1 mentions) Mfp 3d bio atomic force microscope, by Oxford Instruments (1 mentions) Scanasyst air, by Bruker (1 mentions) Poly l lysine (pll), by Ted Pella (1 mentions) Mmafm 2, by Veeco (1 mentions) Peakforce hirs f a tips, by Bruker (1 mentions) Multimode 8 afm, by Bruker (1 mentions) Nanoscope analysis software, by Bruker (1 mentions)

15 protocols using mica surface

AFM Imaging of Biotinylated DNA Origami

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples for AFM imaging were first purified with 100-kDa Amicon spin filters and then incubated with 20-fold excess of streptavidin protein for 1 hour at room temperature. For AFM imaging, 10 μl of samples was deposited onto a freshly cleaved mica surface (Ted Pella, USA) for 1 min. The mica surface was then washed twice with 1× TAE/Mg²⁺ buffer using compressed air. After washing, 60 μl of 1× TAE/Mg²⁺ buffer and 2.5 μl of NiCl₂ solution (0.2 M) were added. The samples were imaged in “ScanAsyst in Fluid” on the Dimension FastScan AFM with ScanAssyst Fluid⁺ probes (both from Bruker, USA). The 2-μm × 2-μm AFM images were scanned at a resolution of 1024 lines with 1024 pixels per line. We analyzed the patterns on around 200 origamis for the statistics of each temporal event.

Liu L., Hong F., Liu H., Zhou X., Jiang S., Šulc P., Jiang J.H, & Yan H. (2022). A localized DNA finite-state machine with temporal resolution. Science Advances, 8(12), eabm9530.

+ Open protocol

+ Expand

AFM Imaging of DNA-Binding on Mica

Check if the same lab product or an alternative is used in the 5 most similar protocols

For AFM imaging, the sample (15 μl) was deposited onto a freshly cleaved mica surface (Ted Pella, Inc.) and left to adsorb for 1 min. A volume of 40 μl of 1× TAE-Mg²⁺ and 2 to 15 μl 100 mM NiCl₂ was added onto the mica, and the sample was scanned on a Veeco 5 Multimode AFM in the Scanasyst in Fluid mode using Scanasyst in fluid+ tips (Veeco, Inc.).

Han D., Qi X., Myhrvold C., Wang B., Dai M., Jiang S., Bates M., Liu Y., An B., Zhang F., Yan H, & Yin P. (2017). Single-stranded DNA and RNA origami. Science (New York, N.Y.), 358(6369), eaao2648.

+ Open protocol

+ Expand

Atomic Force Microscopy of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 5 µL aliquot of the sample was placed on freshly cleaved Mica surface (Ted Pella, Inc). This was allowed to air dry. The samples were then washed with 100 µL of water two times, with drying between each wash. Samples were imaged in air in tapping mode on a Multimode AFM with Nanoscope IIIa controller (Veeco) using oxide-sharpened silicon probes having a resonance frequency of 265–400 kHz (MikroMasch-NSC15, force constant: 40 N/m). All AFM images were obtained at room temperature.

Gleaton J., Curtis R.W, & Chmielewski J. (2021). Formation of Microcages from a Collagen Mimetic Peptide via Metal-Ligand Interactions. Molecules, 26(16), 4888.

+ Open protocol

+ Expand

Mica Surface Preparation for AFM Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

A freshly cleaved mica surface (Ted Pella, Inc.) was treated with poly-L-lysine (1 mg/mL) before sample deposition. 5 μL of the annealed sample was spotted onto poly-L-lysine modified mica and left to adsorb for 2 minutes. The sample was washed with ddH₂O 3 times. Then the sample was air-dried before being mounted on to the microscope. The imaging was performed in the tapping-in-air mode using a NanoScope IV (Digital Instruments), with commercial cantilevers with Si₃N₄ tips for air mode.

Udomprasert A., Bongiovanni M.N., Sha R., Sherman W.B., Wang T., Arora P.S., Canary J.W., Gras S.L, & Seeman N.C. (2014). Amyloid fibrils nucleated and organized by DNA origami constructions. Nature nanotechnology, 9(7), 537-541.

+ Open protocol

+ Expand

Atomic Force Microscopy of DNA Origami

Check if the same lab product or an alternative is used in the 5 most similar protocols

Imaging was performed using tapping mode on a Multimode VIII atomic force microscope (AFM) with an E-scanner (Bruker). Imaging was performed in TAE/Mg²⁺ buffer solution with DNP-S oxide-sharpened silicon nitride cantilevers and SNL sharp nitride levers (Bruker Probes) using resonance frequencies between 7–9 kHz of the narrow 100 μm, 0.38 N/m force constant cantilever. After self-assembly of the origami structure ≈20 μl of TAE/Mg²⁺ buffer solution was deposited onto a freshly cleaved mica surface (Ted Pella) glued to a metal puck (Ted Pella). After 30 s the mica surface was dried using a gentle stream of N₂ and 5 μl of the origami solution was deposited onto the mica surface. After another 30 s, 30 μl of additional buffer solution was added to the sample. Imaging parameters were optimized for best image quality while maintaining the highest possible setpoint to minimize damage to the samples. Images were post-processed by subtracting a 1st order polynomial from each scan line. Drive amplitudes were approximately 0.11 V, integral gains ≈2, and proportional gains ≈4.

Jungmann R., Avendano M.S., Woehrstein J.B., Dai M., Shih W.M, & Yin P. (2014). Multiplexed 3D Cellular Super-Resolution Imaging with DNA-PAINT and Exchange-PAINT. Nature methods, 11(3), 313-318.

+ Open protocol

+ Expand

Mica Surface Preparation for AFM Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Atomic Force Microscopy of DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

In all, 2 µL of sample (5 nM) was dropped on a freshly cleaved mica surface (Ted Pella, Inc.). After 2 min, the sample was mixed with 50 µL 1xTAE-Mg²⁺ buffer and 2 µL 100 mM Ni²⁺. The samples were scanned with SCANASYST-FLUID probes (Bruker) in “ScanAsyst in fluid” mode using a Bruker Multimode V8 Scanning Probe Microscope (Bruker, Germany).

Fu X., Ke G., Peng F., Hu X., Li J., Shi Y., Kong G., Zhang X.B, & Tan W. (2020). Size-selective molecular recognition based on a confined DNA molecular sieve using cavity-tunable framework nucleic acids. Nature Communications, 11, 1518.

+ Open protocol

+ Expand

Atomic Force Microscopy of Synthesized Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

MFP 3D BIO atomic force microscope, purchased from Asylum Research/Oxford Instruments was operating in Semi-Contact mode in order to visualize topography of synthesized materials and for the evaluation of flakes’ thickness. Samples for the AFM examination were prepared in the form of water suspension. Such suspension was pipetted mica surface (Ted Pella). Subsequently the samples were stored in a Memmert VO 200 vacuum dryer for 1 h at 50 mbar prior to imaging.
Surface visualization was performed in the ambient conditions (relative humidity of 18% and temperature of 22 °C). AC 160 TS R3 (Olympus) scanning probe (spring constant of c.a. 26 N/m and radius of c.a. 10 nm–according to the microscope’s producer) was mounted in the microscope. Prior to visualization, the scanning probe was calibrated with Auto Tune method in order to set its drive frequency (c.a. 320 kHz). AFM topography images were recorded at 0.6 Hz scan rate. Analysis of the recorded topographical maps were performed using IgorPro ver. 6.17.

Chlanda A., Kowiorski K., Małek M., Kijeńska-Gawrońska E., Bil M., Djas M., Strachowski T., Swieszkowski W, & Lipińska L. (2021). Morphology and Chemical Purity of Water Suspension of Graphene Oxide FLAKES Aged for 14 Months in Ambient Conditions. A Preliminary Study. Materials, 14(15), 4108.

+ Open protocol

+ Expand

Self-Replication Imaging with AFM

Check if the same lab product or an alternative is used in the 5 most similar protocols

AFM imaging was performed in peakForce mode in air for the self-replication cycling. Two to five microliters of a diluted sample was deposited on a clean mica surface (Ted Pella, Inc.) for 1 min. Unless specified, the sample and mica were incubated at 48 °C for 30 min and the deposition was conducted at 48 °C. The mica was then washed with 50 to 70 μL of double-distilled H₂O three times and subsequently dried using N₂. PeakForce in air mode was performed on the Nansocope V Multimode 8 scanning probe microscope (PeakForce QNM Software, ScanAsyst-HR accessory). Silicon nitride tips (ScanAsyst-Air; Bruker Nano, Inc.) were used for the Nanoscope V Multimode 8 SPM.

Zhou F., Sha R., Ni H., Seeman N, & Chaikin P. (2021). Mutations in artificial self-replicating tiles: A step toward Darwinian evolution. Proceedings of the National Academy of Sciences of the United States of America, 118(50), e2111193118.

+ Open protocol

+ Expand

Adsorption of Small RNA Particles on Mica

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mica surface (Ted Pella, Inc.) was pre-treated with poly-L-lysine (0.1% w/v, Ted Pella, Inc.) to increase the adsorption of small RNA particles. 20 µL poly-l-lysine (5 µg/ml) was added onto freshly cleaved mica substrate and was incubated for 30 s. Then the surface was washed by 100 µL H₂O and dried by compressed air. Annealed RNA solution was diluted to 50 nM and a drop of 5 µL was deposited onto pre-treated mica and incubated for 2 min. 20 µL TAE/Mg²⁺ buffer was further added. Imaging was performed in a fluid cell under tapping mode on a Multimode AFM (MMAFM 2, Veeco) with SNL-10 probe (Veeco, Inc.).

Li M., Zheng M., Wu S., Tian C., Liu D., Weizmann Y., Jiang W., Wang G, & Mao C. (2018). In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs. Nature Communications, 9, 2196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!