Timm23

Manufactured by BD

Sourced in United States

TIMM23 is a laboratory equipment product manufactured by BD. It is a device used for the detection and analysis of specific molecular targets within biological samples. The core function of TIMM23 is to facilitate the identification and quantification of target analytes, such as proteins or nucleic acids, through the application of analytical techniques.

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Lab products found in correlation

Tomm20, by Cell Signaling Technology (1 mentions) Map1lc3b lc3b, by Cell Signaling Technology (1 mentions) Siah1, by Abcam (1 mentions) Hsp60, by BD (1 mentions) Micu1, by Abcam (1 mentions) Atp5a, by BD (1 mentions) Actin, by Abcam (1 mentions)

2 protocols using timm23

Mitochondrial Protein Characterization by Western Blotting

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Western blotting was performed as described previously (19). Primary antibodies against PINK1 (#6946), PRKN (#4211), TOMM20 (#42406), COX4I1/Cox IV (#4850), MFN1 (#14739), MFN2 (#9482), STUB1/CHIP (#2080 S), SMURF1 (#2174), phospho-ubiquitin (Ser65, #62802), and MAP1LC3B/LC3B (#3868) were purchased from Cell Signaling (Beverly, MA, USA); SQSTM1/p62 (#sc-48402), ACTB (#sc-47778), and SLC5A2/SGLT2 (#sc-393350) from Santa Cruz Biotechnology (Dallas, TX, USA); phospho-ubiquitin (Ser65, ABS1513-I) from Merck; TIMM23 (#611223) from BD Biosciences; MTCO2/Cox II (#ab110258), MUL1 (#ab209263), and SIAH1 (#ab2237) from Abcam (Shanghai, China).

Zhou J., Feng J., Wu Y., Dai H.Q., Zhu G.Z., Chen P.H., Wang L.M., Lu G., Liao X.W., Lu P.Z., Su W.J., Hooi S.C., Ye X.P., Shen H.M., Peng T, & Lu G.D. (2022). Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy. Experimental & Molecular Medicine, 54(11), 2007-2021.

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Immunoblot Antibody Validation

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Immunoblots were performed using antibodies from Sigma (Micu1, Mcu, actin), Abcam (HSP60, ATP5a), and BD Biosciences (TIMM23). Antibodies against Micu2 and EMRE were produced in collaboration with Bethyl.

Bick A.G., Wakimoto H., Kamer K.J., Sancak Y., Goldberger O., Axelsson A., DeLaughter D.M., Gorham J.M., Mootha V.K., Seidman J.G, & Seidman C.E. (2017). Cardiovascular homeostasis dependence on MICU2, a regulatory subunit of the mitochondrial calcium uniporter. Proceedings of the National Academy of Sciences of the United States of America, 114(43), E9096-E9104.

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