Antipyrine, cimetidine, clotrimazole, N-desmethyl-tamoxifen hydrochloride, furosemide, hydrochlorothiazide, ketoprofen, maleic acid, (+/−)-metoprolol-(+)-tartrate, (+/−)-norverapamil hydrochloride, piroxicam, tamoxifen, terbutaline hemisulfate and (+/−)-verapamil hydrochloride 99% were purchased from Sigma-Aldrich (St. Louis, MO, USA); (+/−)-propranolol hydrochloride and ranitidine hydrochloride were obtained from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany), and carbamazepine was purchased from Acros Organics (New Jersey, USA). Midazolam and α-hydroxyMidazolam were purchased from Lipomed AG (Arlesheim, Switzerland), and agar, calcium chloride dihydrate, glucose hydrate, magnesium chloride hexahydrate, potassium chloride, sodium chloride, sodium hydroxide, sodium phosphate monobasic and sodium hydrogencarbonate were obtained from Hänseler AG (Herisau, Switzerland). Sodium taurocholate was purchased from Prodotti Chimici e Alimentari S.p.A., (Basaluzzo, Italy) and lecithin (grade EPCS > 98% phospholipids) was obtained from Lipoid GmbH (Ludwigshafen, Germany).
Antipyrine
Manufactured by Merck Group
Sourced in United States
Antipyrine is a chemical substance used as a laboratory reagent. It is a white crystalline powder that is soluble in water, alcohol, and other organic solvents. Antipyrine is commonly used in various analytical and research applications in chemistry and biology laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Cimetidine, by Merck Group (3 mentions)
Ketoprofen, by Merck Group (3 mentions)
Furosemide, by Merck Group (3 mentions)
Metoprolol, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Propranolol, by Merck Group (3 mentions)
Piroxicam, by Merck Group (2 mentions)
Hydrochlorothiazide, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Formic acid, by Biosolve (2 mentions)
Digoxin, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Diclofenac, by Merck Group (2 mentions)
Buspirone, by Merck Group (2 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
Phenacetin, by Merck Group (2 mentions)
Ibuprofen, by Merck Group (2 mentions)
Maleic acid, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Clotrimazole, by Merck Group (1 mentions)
22 protocols using antipyrine
1
In Vitro Solubility Screening Compounds
2
Analytical Standards for Pharmacological Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents were of UPLC grade. Acetonitrile (MeCN) was purchased from Merck. Methanol was from Avantor Performance Materials Poland. Purified water was obtained from a Milli-Q integral water purification system. Dimethyl sulfoxide (DMSO) was supplied by Scharlau, and formic acid was from BioSolve. Antipyrine, hyperforin dicyclohexylammonium salt and bovine serum albumin (BSA) were obtained from Sigma-Aldrich, and Antipyrine-d3 from HPC Standards. Valerenic acid was purchased from Extrasynthese and PhytoLab, and hypericin was from Carbosynth. Warfarin was purchased from Toronto Research Chemicals, and digoxin from Sigma-Aldrich.
3
Liquid Chromatography-Mass Spectrometry of Zearalenone Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatography–mass spectrometry (LC-MS)-grade acetonitrile (ACN) and methanol (MeOH) were obtained from Sigma-Aldrich, and water ( , LC-MS grade) was purchased from VWR International GmbH. Ammonium fluoride (LC-MS grade) was obtained from Honeywell Fluka. ZEN, , , , , and were obtained from either Romerlabs or Sigma-Aldrich, whereas ZEN-14-GlcA, , and were synthesized at the Technical University of Vienna and kindly provided by Dr. Mikula (Mikula et al. 2012 (link), 2013 (link)). ZEN-14-Sulf was a kind gift from Prof. Berthiller from the University of Natural Resources and Life Sciences, Vienna (Vendl et al. 2010 (link)). Antipyrine was obtained from Sigma-Aldrich. All reference standards were dissolved in ACN. ZEN and its metabolites were combined manually to a multianalyte calibration solution (each analyte at a concentration of ). All single and combined analytes were stored at . ZEN for transfusion experiments was obtained from Toronto Research Chemicals (purity 98.9%).
4
Permeability Evaluation of Five Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human iPS-IECMs, which were cultured on the cell culture inserts, were preincubated with HBSS adjusted to pH 7.4 with 10-mM HEPES for 10 minutes at 37°C. The 1.5 mL of HBSS containing 1-μM Antipyrine, metoprolol, fexofenadine, atenolol, and pravastatin was added to the apical chamber, and 2.6 mL of HBSS containing 0.1% DMSO was also added to the basolateral chamber. Antipyrine, metoprolol, fexofenadine, atenolol, and pravastatin were purchased from Sigma-Aldrich. After 30-, 60-, 90-, and 120-minute incubation at 37°C, the solution was collected from the basolateral chamber. Antipyrine, metoprolol, fexofenadine, atenolol, and pravastatin concentration was measured by UPLC-MS/MS. The Fa values of Antipyrine, metoprolol, fexofenadine, atenolol, and pravastatin were cited from previous studies.35 (link), 36 (link), 37 (link)
5
Lipoxygenase Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N-(1-Naphthyl) ethylene-diamine dihydrochloride, methanol and acetonitrile were from Merck. 5-LOX from Glycine max (Type V; EC 1.13.11.12), linoleic acid, diacetyl monoxime, antipyrine, quercetin, sulphanilamide, sodium phosphate, phosphoric acid, thiazolyl blue tetrazolium bromide (MTT), LPS from Escherichia coli, N-[naphth-1-yl]ethylenediamine dihydrochloride, dimethyl sulfoxide (DMSO), l -arginine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium nitroprusside dihydrate (SNP) was acquired from Riedel-de Haën (St. Louis, MO, USA). The water was treated in a Milli-Q water purification system (Millipore, Bedford, MA, USA). Formic acid was acquired from Labkem (Barcelona, Spain). Murine macrophage cell line RAW 264.7 were purchased from the American Type Culture Collection (LGC Standards S.L.U., Barcelona, Spain). Dulbecco’s Modified Eagle Medium with GlutaMAX™ supplement (DMEM + GlutaMAX), fetal bovine serum (FBS) and penicillin-streptomycin solution were obtained from GIBCO, Invitrogen (Grand Island, NY, USA). 3-O-Caffeoylquinic acid and 5-O-caffeoylquinic acid were obtained from Biopurify Phytochemicals Ltd. (Chengdu, China). Catechin, epiCatechin, vitexin, and isovitexin were purchased from Extrasynthese (Genay, France). Vicenin-2 was obtained from HWI Analytik GMBH (Rülzheim, Germany).
6
Biochemical Assay for Kynurenine Pathway
7
Microsomal Enzyme Inhibition Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dimethyl sulfoxide (DMSO, UPLC/MS grade), albendazole, buspirone, propranolol, loperamide, diclofenac, carbamazepine, antipyrine, potassium phosphate monobasic, and potassium phosphate dibasic were purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile (ACN, UPLC/MS grade) was purchased from Fisher Scientific (Hampton, NH). Gentest rat (Sprague–Dawley) liver microsomes (male, pooled, 20 mg/mL, Catalog #: 452501), Gentest NADPH Regenerating Solution A (Catalog #: 451220) and B (Catalog #: 451200), Axygen reservoirs (low-profile, Catalog #:RES-SW384-LP; high-profile, Catalog #: RES-SW384-HP) for holding reagents, and CoStar assay block plates (Catalog #: 3959) for microsomal incubation were purchased from Corning Inc. (Corning, NY). LC–MS/MS analysis plates (Catalog #: 186002643) were purchased from Waters Inc. (Milford, MA).
8
Quantification of Antipyrine by HPLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antipyrine (Sigma Aldrich, Steinheim, Germany) was quantified by high-performance liquid chromatography (HPLC) according to Annola et al. [77 (link)]. Samples were analyzed by a liquid chromatography system (Knauer, Germany, autosampler 3950, degasser 5050, pump 1050, and UV-detector 2500 wavelength of 255 nm) and separated before by an Aquasil C18 (150 × 2.1, 5 µm) column (Thermo Scientific, Vienna, Austria). Acetonitrile and 20 mM K2HPO4 (Merck, Darmstadt, Germany 1:1 mixture) was applied as mobile phase by an isocratic flow of 0.2 ml/min at room temperature.
9
Protein Purification and Cross-linking
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents including aspartate, carbamoyl phosphate, antipyrine, and diacetyl monooxime were obtained from Sigma, pETite vector and E. coli Hi-Control BL21(DE3) cells from Lucigen, and BS3 (bis (sulfosuccinimidyl) suberate) cross-linker from Thermo Fisher Scientific.
10
In Vitro Brain-Plasma Distribution Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemicals and reagents, including Altanserin, antipyrine, atenolol, buspirone, cimetidine, citalopram, N-desmethylclozapine, diphenhydramine, doxepin, fluoxetine, gabapentin, indomethacin, metoclopramide, propranolol, risperidone, and Way-100635 were purchased from Sigma Aldrich (St. Louise, Missouri, US). The calibration curves and matrices for the in vitro equilibrium brain-to-plasma distribution experiments, were prepared using BSA purchased from Sigma Aldrich (≥ 98%, St. Louise, Missouri, US, Product no.: A7906 Lot. No.: SLCH8448) and brain homogenate from BioIVT (Westbury, NY, US, Product no. RAT00BRAINMZA, lot no. RAT415237). For the in vitro binding experiments, BSA (purity > 98%) was obtained from Beijing SeaskyBio Technology Co. Ltd. (Beijing, North China, China. Product no.: BSAS, Lot.: 541), and Sprague–Dawley brain homogenate was purchased from BioIVT (Westbury, NY, US, Product no. RAT00BRAINMZA, Lot no.: RAT 472873) and Shanghai Biotechnology Co. Ltd (Shanghai, East China, China. Lot.: 20,221,213). Other chemicals and solvents were of analytical grade and obtained from a commercial supplier.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!