The vitrification of the samples was carried out in a homemade vitrification system. The chamber was held at 22 °C and the relative humidity at 80%. A 5 µL drop of the sample (1–2 mg·mL−1) was deposited onto a lacey carbon film covered grid ((Ted Pella, Redding, CA, USA) rendered hydrophilic using an ELMO glow discharge unit (Cordouan Technologies, Bordeaux, France). The grid was automatically blotted to form a thin film which was plunged in liquid ethane held at −190 °C by liquid nitrogen. That way, a vitrified film was obtained, in which the native structure of the vesicles was preserved. The grid was mounted onto a cryo holder (Gatan 626, Pleasanton, CA, USA) and observed under low dose conditions in a Tecnai G2 microscope (FEI, Eindhoven, Netherland) at 200 kV. Images were acquired using an Eagle slow scan CCD camera (FEI).
Tecnai g2 microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai G2 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It features advanced optics and a high-brightness electron gun, enabling the acquisition of detailed images at magnifications up to 1.2 million times. The Tecnai G2 is capable of operating at acceleration voltages up to 200 kV, making it suitable for a variety of applications, including materials science, nanotechnology, and biological research.
Automatically generated - may contain errors
Lab products found in correlation
Eagle slow scan ccd camera, by Thermo Fisher Scientific (2 mentions)
Continuous carbon electron microscopy grids, by Agar Scientific (2 mentions)
Gatan 626, by Ametek (1 mentions)
Asap 2020 volumetric adsorption analyzer, by Micromeritics (1 mentions)
D max 2400, by Rigaku (1 mentions)
Lambda 750, by PerkinElmer (1 mentions)
Xsam800, by Shimadzu (1 mentions)
Invia raman microscope, by Renishaw (1 mentions)
Merlin fe sem, by Zeiss (1 mentions)
S 4800, by Hitachi (1 mentions)
Asap 2020, by Micromeritics (1 mentions)
Sw28 rotor, by Beckman Coulter (1 mentions)
V 550 spectrophotometer, by Jasco (1 mentions)
Tecnai g2 spirit microscope, by Ametek (1 mentions)
D max 2500, by Rigaku (1 mentions)
D5000, by Siemens (1 mentions)
Axis ultra dld, by Shimadzu (1 mentions)
Uc7 ultramicrotome, by Leica camera (1 mentions)
Veleta camera, by Olympus (1 mentions)
Digital micrograph, by Ametek (1 mentions)
39 protocols using tecnai g2 microscope
1
Vitrification of Vesicle Samples
2
Characterization of HP-β-CD/CC and SBA-16 Samples
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SEM images of the prepared HP-β-CD/CC and SBA-16 samples were taken with S-4800 type microscope (Hitachi, Japan) in high-vacuum mode. Transmission electron microscopy (TEM) images of the particles were recorded on a Tecnai G2 microscope (FEI, the Netherlands) operated at an accelerating voltage of 200 kV. The particle size distribution was measured by DLS using a Zetasizer Nano sizer (Malvern, UK). Nitrogen sorption isotherms and pore characterization of the prepared samples were recorded at −196°C using an ASAP 2020 volumetric adsorption analyzer (Micromeritics, USA). The prepared HP-β-CD/CC and SBA-16 samples were degassed at 100°C for 6 hours prior to measurement, while the samples loaded with IRB and pure IRB powder were degassed at 40°C overnight in the degas port to avoid possible thermal degradation. The specific surface area was calculated by the standard Brunauer–Emmett–Teller (BET) model. The pore size distribution was determined according to the Barrett–Joyner–Halenda (BJH) method.
3
Comprehensive Nanomaterial Characterization
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SEM images and EDX spectroscopy elemental mapping analyses were obtained with a FEI model Quanta microscope at accelerating voltages of 15–30 kV. TEM images were obtained with a FEI Tecnai G2 microscope. XRD patterns were obtained with a Rigaku D/MAX 2400 instrument with a Cu source. XPS analyses were conducted with an Al Kα X-ray source (1486.6 eV) and a hemispherical concentric analyzer (CLAM2–VG Microtech). Fourier Transform Infrared Spectrometer (FTIR) (Brook Vertex 70) was used to identify the related functional groups in the catalyst.
4
Electron Microscopy of RVFV Infection
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Huh7 cells (2 × 106) were infected with RVFV WT (BJ01), rWT or rescued virus lacking the NSs gene (r△NSs-eGFP) at an MOI of 1 for 24 h. Cells were washed three times with PBS, fixed with 2.5% (w/v) glutaraldehyde in 0.1 M sodium chloride and and processed for Electron Microscopy (EM). The viral particles were observed under a transmission electron microscope (FEI Tecnai G2 microscope at 200 kV).
5
Cryogenic Electron Tomography of Reovirus Infection
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Semithick (~300-nm) Tokuyasu cryosections of reovirus-infected cells were collected on copper grids with parallel bars. Four single-axis tilt series were obtained automatically between −63° and +60° with an angular interval of 1.5°. Images were recorded on an Eagle 4k-by-4k slow-scan charge-coupled device (FEI, Eindhoven, The Netherlands) using FEI software and a Tecnai G2 microscope (FEI) operating at 200 kV. Images were aligned, and tomograms were reconstructed using the IMOD software package (56 (link)). The tomogram with best contrast was segmented and processed for 3D visualization with Amira. Tomograms were subjected to noise filtering and automated segmentation to visualize membranes (57 (link)).
6
Characterization of Polymeric Gene Delivery Complexes
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GPP morphology was evaluated in a negative staining mode by transmission
electron microscopy (TEM, Tecnai G2 microscope (FEI)), using 1% w/v
aqueous uranyl acetate staining. Samples were prepared in PBS at a
final polymer concentration of 0.25 mg mL–1 and
at the same N/P ratios used for the DLS analysis. Samples were deposited
on a small holey carbon-coated support grid (400 mesh), and the solvent
was allowed to dry at room temperature. The average diameter of GPPs
and the percentage of spherical, rod-, or toroid-shaped complexes
were evaluated by ImageJ software v.1.51 by measuring 50 individual
GPPs.
electron microscopy (TEM, Tecnai G2 microscope (FEI)), using 1% w/v
aqueous uranyl acetate staining. Samples were prepared in PBS at a
final polymer concentration of 0.25 mg mL–1 and
at the same N/P ratios used for the DLS analysis. Samples were deposited
on a small holey carbon-coated support grid (400 mesh), and the solvent
was allowed to dry at room temperature. The average diameter of GPPs
and the percentage of spherical, rod-, or toroid-shaped complexes
were evaluated by ImageJ software v.1.51 by measuring 50 individual
GPPs.
7
Electron Microscopy of Adenovirus Morphology
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The morphology and structure of naked OV and PC-OC were examined by transmission electron microscopy (TEM) using the Tecnai G2 microscope (FEI). Samples, prepared as described in “Formulation of unlabeled and fluorescently labeled Gal32-b-Agm29 coated Ad5/3-D24-ICOSL adenovirus”, were deposited on a small holey carbon-coated support grid (400 mesh), and the solvent was allowed to dry at room temperature. Samples were negatively stained with 1% w/v aqueous uranyl acetate solution prior to imaging. TEM images were captured by rotating the sample from +60° to −60° and collected every 2° of rotation.
8
Characterization of Nanomaterial Samples
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The high-resolution TEM images and SAED pattern were taken with an FEI Tecnai G2 microscope. The optical transmittances were measured by using a Perkin-Elmer model Lambda 750 UV–vis–NIR spectrophotometer. The Raman spectra were collected with a Renishaw InVia Raman microscope using a 514-nm laser beam (20 mW; 1 cm−1). XPS was performed on a Kratos XSAM800 using Al Ka radiation (144 W, 12 mA, 12 kV).
9
Detailed Characterization of Prepared Samples
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The morphology
of the prepared samples
was characterized by SEM (Hitachi S-4800). The FESEM images were obtained
with Carl Zeiss Merlin FESEM at an operating voltage of 5 kV. The
TEM images were obtained on a FEI Tecnai G2 microscope, and the samples
were dispersed in ethanol and dropped on a copper grid. The XRD patterns
were recorded on a Rigaku-SmartLab X-ray diffractometer with Cu Kα
radiation (λ = 1.5418 Å) operating at 40 kV, 30 mA. The
data were collected from 10° to 90° with the scan rate of
30 min–1 and steps of 0.02. The nitrogen adsorption–desorption
measurements of the samples were carried out at −196 °C,
and the surface area (SBET) values were
calculated using the standard Brunauer–Emmett–Teller
(BET) equation (Micromeritics ASAP 2020). The total pore volume (Vtotal) of the samples was calculated at the
maximum relative pressure of P/P0 = 0.995. The micropore (Vmicro) and mesopore (Vmeso) volumes were calculated
using the Dubinin–Radushkevich analysis.
of the prepared samples
was characterized by SEM (Hitachi S-4800). The FESEM images were obtained
with Carl Zeiss Merlin FESEM at an operating voltage of 5 kV. The
TEM images were obtained on a FEI Tecnai G2 microscope, and the samples
were dispersed in ethanol and dropped on a copper grid. The XRD patterns
were recorded on a Rigaku-SmartLab X-ray diffractometer with Cu Kα
radiation (λ = 1.5418 Å) operating at 40 kV, 30 mA. The
data were collected from 10° to 90° with the scan rate of
30 min–1 and steps of 0.02. The nitrogen adsorption–desorption
measurements of the samples were carried out at −196 °C,
and the surface area (SBET) values were
calculated using the standard Brunauer–Emmett–Teller
(BET) equation (Micromeritics ASAP 2020). The total pore volume (Vtotal) of the samples was calculated at the
maximum relative pressure of P/P0 = 0.995. The micropore (Vmicro) and mesopore (Vmeso) volumes were calculated
using the Dubinin–Radushkevich analysis.
10
Phage Morphology Analysis by TEM
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The morphology of phages PP47, PP81 and Q19 was analysed by transmission electron microscopy (TEM). Phage suspension (~109 pfu/mL) was purified by ultracentrifugation in a CsCl gradient (rotor SW28, Beckman, 22,000× g for 40 min at 4 °C), dialysed against the phage buffer, placed on individual copper grids, negatively stained with 1% uranyl acetate and examined using an FEI Tecnai G2 microscope at 100kV acceleration voltage. The dimensions were averaged among ~20 individually measured particles.
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