Candida parapsilosis atcc22019

LH was bought from National Institutes of Food and Drug Control of China. RPMI-1640 medium powder, 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT), 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), menadione, morpholinepropanesulfonic acid (MOPS), and dibutyryl-cAMP (db-cAMP) were bought from Sigma-Aldrich (Shanghai, China). LH was dissolved in DMSO and stored at -20°C.
C. albicans SC5314, C. albicans ATCC90028, Candida glabrata ATCC2001, Candida parapsilosis ATCC22019, and Candida tropicalis ATCC7349 bought from China General Microbiological Culture Collection Center (CGMCC) were maintained on yeast extract-peptone-dextrose (YPD) agar medium (1% yeast extract, 2% peptone, 2% dextrose, and 2% agar). Before each test, a colony was picked up and transferred into 5 mL YPD medium in a sterile tube and incubated overnight at 28°C with rotation (140 rpm).

C. albicans SC5314, C. albicans ATCC10231, Candida glabrata ATCC2001, and Candida parapsilosis ATCC22019 were obtained from China General Microbiological Culture Collection Center (CGMCC). All these strains were maintained on YPD (yeast extract 1%, peptone 2%, dextrose 2%) medium and stored at -80°C. Before each test, a colony from fungal strains subcultured twice on YPD agar was transferred into YPD medium and incubated overnight at 30°C with a speed of 140 rpm for propagation.
Dioscin was bought from Solarbio Life Sciences. N-acetyl-D-glucosamine (GlcNAc), XTT, MTT, menadione, propidium iodide (PI), and RPMI-1640 medium were bought from Sigma.