Endoxifen

The Bradford protein assay and the chemiluminescence reagent for Western Blot Clarity Western ECL Substrate were obtained from Bio-Rad Laboratories (Hercules, CA, USA). The anti-phospho-p38 (Thr180/Tyr182) (cat. n° 4511), anti-p38 (cat. n° 8690), and anti-poly[ADP-ribose] polymerase 1 (PARP-1) (cat. n° 9542) antibodies were purchased from Cell Signalling Technology Inc. (Beverly, MA, USA). The anti-α-tubulin (cat. n° T6074) and anti-COX-IV (cat. n° ZRB1593) antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-PP2A (cat. n° sc-56954), anti-ERα (cat. n° sc-8005), and anti-ERβ (cat. n° sc-373853) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-NGB antibody (cat. n° MABN369) was purchased from Merck Millipore (Darmstadt, D). The ERα inhibitor endoxifen and the ERβ inhibitor (R,R)-5,11-Diethyl-5,6,11,12-tetrahydro-2,8-chrysenediol ((R,R)-THC) were purchased from Tocris (Ballwin, MO, USA). All the other products were from Sigma-Aldrich. Analytical and reagent-grade products were used without further purification.

The Bradford protein assay and the chemiluminescence reagents for Western blot Clarity Western ECL Substrate were obtained from Bio-Rad Laboratories (Hercules, CA, USA). The anti-phospho-ERα (pERα Ser118) antibody and anti-phospho-AKT (pAKT Ser473) were purchased from Cell Signalling Technology Inc. (Beverly, MA, USA). The anti-α-tubulin and anti-NGB antibodies were purchased from Merck (Darmstadt, D). Specific antibodies against AKT, ERα (HC20), and poly [ADP-ribose] polymerase 1 (PARP-1) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The ERα inhibitor endoxifen was purchased from Tocris (Ballwin, MO, USA). All the other products were from Merck. Analytical and reagent grade products were used without further purification.