Longgmr gal4

Manufactured by Bloomington Drosophila Stock Center

Sourced in Japan

The LongGMR-Gal4 is a genetic tool used in Drosophila research. It is a Gal4 driver that expresses the Gal4 transcription factor in the majority of photoreceptor cells in the fly's compound eye. This allows for the targeted expression of genes of interest in these cells for various experimental purposes.

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Lab products found in correlation

Pros gal4, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4, by Bloomington Drosophila Stock Center (1 mentions) Repo gal4, by Bloomington Drosophila Stock Center (1 mentions) Act5c gal4, by Bloomington Drosophila Stock Center (1 mentions) Nanos gal4 vp16, by Bloomington Drosophila Stock Center (1 mentions) Tubp gal4, by Bloomington Drosophila Stock Center (1 mentions)

4 protocols using longgmr gal4

Genetic Manipulation of Wingless Signaling

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All crosses and staging were performed at 25 °C. Pupal development was expressed as hours after puparium formation (APF) where white pre-pupae were defined as 0 h APF. Stocks used were: GMR.wg ((Wehrli and Tomlinson, 1998 (link))), Tub-α1 > w + > wg (Wehrli and Tomlinson, 1998 (link)) UAS-GFP (Johnston and Sanders, 2003 (link)), UAS-arm* (Zecca et al., 1996 (link)) esg-LacZ, wg-lacZ, UAS-Esg-RNAi (TRiP stock BL# 28514), pros-Gal4 (gift of Tiffany Cook) LongGMR-Gal4 (Wernet et al., 2003 (link)) (All from Bloomington Stock Center). GMR.flip (generated by Y. Mavromatakis), UAS-wg-RNAi (Gift from G. Struhl), d-APC^Q8, elav(C155)-Gal4 (Ahmed et al., 1998 (link)).

Kumar S.R., Patel H, & Tomlinson A. (2015). Wingless mediated apoptosis: How cone cells direct the death of peripheral ommatidia in the developing Drosophila eye. Developmental biology, 407(2), 183-194.

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Drosophila Genetic Toolkit Protocols

Cited 1 time

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Flies were maintained using standard conditions. Fly lines repo-Gal4 (BDSC_7415), longGMR-Gal4 (BDSC_8121), elav-Gal4 (BDSC_8760), Hdc^MB07212 (BDSC_25260), R10D10-Gal4 (BDSC_69558), R32H04-Gal4 (BDSC_49734), R29A12-Gal4 (BDSC_49478), and R19C02-Gal4 (BDSC_49282) were provided by the Bloomington Drosophila Stock Center. The mCD8-GFP and 3XPax3-GFP markers and the carT^43A mutant have been described^{25 (link)}. UAS-WNK-RNAi and UAS-fray-RNAi transgenes and their effectiveness in knocking down the relevant target have been described^{10 (link)}. The UAS-Ncc69-RNAi line (VDRC KK106499) was obtained from the Vienna Drosophila Resource Center (VDRC). The Ncc69^r1, Ncc69^r2 and Gli-Gal4; UAS-Ncc69 flies^{13 (link)} were a gift from Dr. William Leiserson (Yale University, New Haven, CT). The MZ709-Gal4 line was a gift from Dr. Hong-Sheng Li (University of Massachusetts Medical Center, Worcester, MA).

Stenesen D., Moehlman A.T., Schellinger J.N., Rodan A.R, & Krämer H. (2019). The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system. Scientific Reports, 9, 2475.

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Drosophila Genetic Toolkit Utilization

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The following fly stocks were obtained from Bloomington Stock Center: DJ667 GAL4 (stock #8171), Act5C-GAL4 (stock #4414), tubP-GAL4 (stock #5138), longGMR-GAL4 (stock 8605), nanos-GAL4:VP16 (stock #7312).

Jones T.I., Parilla M, & Jones P.L. (2016). Transgenic Drosophila for Investigating DUX4 and FRG1, Two Genes Associated with Facioscapulohumeral Muscular Dystrophy (FSHD). PLoS ONE, 11(3), e0150938.

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Drosophila Genetic Manipulation Protocols

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Drosophila were grown at 20–25°C on standard cornmeal–glucose–agar–yeast medium either in the laboratory with room light or in an incubator without light. The following fly stocks were used: Rh1-Gal4 (Chihiro Hama, Kyoto Sangyo University, Japan), longGMR-Gal4 (Bloomington Drosophila Stock Center No. 8605, Bloomington, IN; indicated as BL8605 in the following stocks), coinFLP-Gal4 (BL58751), y w; GMR-hid FRT40A/CyO; ey-Gal4 UAS-FLP/TM6B flies (BL5250), UAS-Rab11RNAi^pWIZ, (Satoh et al., 2005 (link)), UAS-Strat RNAi^GD10605 (Vienna Drosophila Resource Center No. 45715, Vienna, Austria; indicated as v45715 in the following stocks), Rab35RNAi^JF02978 (BL28342), Rab35RNAi^KK108660 (v101361), Rab10^null /FM7, Rab35^null /FM7 (gifts from Hiesinger), and UAS-tagBFP2::FLAG::Rab35 (produced in the present study).
Strat^null with RFP FRT40A/CyO and Strat^WT with RFP FRT40A/CyO were kindly provided by Le Borgne (Bellec et al., 2018 ) and RFP was removed using Cre recombinase (BL106201). Males of Strat^null FRT40A/CyOGFP and Strat^WT FRT40A/CyOGFP were crossed with y w eyFLP; RFP FRT40A/CyOGFP to generate mosaic eyes. Rab8¹ (BL26173) was combined with FRT80B. Males of Rab8¹ FRT80B/TM6B were crossed with y w eyFLP; RFP FRT80B to generate mosaic eyes.

Ochi Y., Yamashita H., Yamada Y., Satoh T, & Satoh A.K. (2022). Stratum is required for both apical and basolateral transport through stable expression of Rab10 and Rab35 in Drosophila photoreceptors. Molecular Biology of the Cell, 33(10), br17.

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