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3 protocols using aprotinin

1

Characterization of Multidrug Resistance Transporters

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R6G, KTC, ANI, MIC, CTR, CYH, ITC, NR, adenosine triphosphate (ATP), oligomycin (OM), trypsin, phenylmethanesulfonyl fluoride (PMSF), p-tosyl-L-lysine chloromethyl ketone (TLCK), and tosyl phenylalanyl chloromethyl ketone (TPCK) were procured from Sigma Chemical Co. (St. Louis, MO). The protease inhibitors leupeptin, pepstatin A, and aprotinin were purchased from G-Biosciences (MO, USA). FLC was generously provided by Ranbaxy, India. Ascorbic acid (AA) was purchased from SRL (Mumbai, India). Oligonucleotides used in this study were commercially procured from Sigma Genosys, India and are listed in Supplementary Table S4. Anti-GFP monoclonal antibody and anti-mouse secondary antibody were purchased from Santa Cruz Biotechnology Inc. (Texas, USA). The anti-Pma1 (PM ATPase) polyclonal antibody was a gift from Professor Ramon Serrano (Universidad Politecnica de Valencia-CSIC, Valencia, Spain). 3H-radiolabeled FLC (3H-FLC) was purchased from Moravek Biochemicals and Radiochemicals (CA, USA).
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2

Protein Expression and Inhibition Assay

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Rhodamine 6G (R6G), ketoconazole (KTC), anisomycin (ANI), miconazole (MIC), cycloheximide (CYH), itraconazole (ITC), nile red (NR), adenosine triphosphate (ATP), oligomycin, phenylmethanesulfonyl fluoride (PMSF), tosyllysine chloromethyl ketone (TLCK), and tosyl phenylalanyl chloromethyl ketone (TPCK) were procured from Sigma Chemical Co. (St. Louis, MO). The protease inhibitors leupeptin, pepstatin A, and aprotinin were purchased from G-Biosciences (MO, USA). Fluconazole (FLC) was generously provided by Ranbaxy, India. Ascorbic acid (AA) was purchased from SRL (Mumbai, India). Oligonucleotides used in this study were purchasedfrom Sigma Genosys, India and are listed in Supplementary Table S2. Anti-GFP monoclonal antibody and anti-mouse secondary antibody were purchased from Santa Cruz Biotechnology Inc. (Texas, USA).
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3

Comprehensive Biochemical Reagents Protocol

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Itraconazole (ITC), Clotrimazole (CTR), Ketoconazole (KTZ), Miconazole (MCZ) and Voriconazole (VOR), Anisomycin (ANI), Rhodamine 6G (R6G), 4-nitroquinoline 1-oxide (NQO), Adenosine triphosphate (ATP), Oligomycin (OM), DL-Dithiothreitol (DTT), Sorbitol, Phenylmethanesulfonyl fluoride (PMSF), p-Tosyl-L-lysine chloromethyl ketone (TLCK), and Tosyl phenylalanyl chloromethyl ketone (TPCK) were procured from Sigma Chemical Co. (St. Louis, MO). Protease inhibitors leupeptin, aprotinin, Pepstatin were obtained from G-biosciences, MO, USA). Fluconazole (FLC) was a generous gift from Ranbaxy Laboratories, India. Oligonucleotides were procured from Sigma Genosys, India and are listed in Table S2. Anti-GFP monoclonal antibody was purchased from Santa Cruz Biotechnology Inc. (Texas, USA). All other routine chemicals were purchased from Fisher-scientific, Mumbai, India.
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