Dm1000 photonic microscope

Manufactured by Leica

Sourced in France, Germany

The Leica DM1000 is a photonic microscope designed for laboratory use. It features high-quality optics and illumination to provide clear, detailed images of specimens. The core function of the DM1000 is to enable observation and analysis of microscopic samples.

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Lab products found in correlation

Api zym, by bioMérieux (6 mentions) Api 50ch, by bioMérieux (5 mentions) Api 20ne, by bioMérieux (4 mentions) Tecnai g20, by Thermo Fisher Scientific (4 mentions) Color gram 2 kit, by bioMérieux (3 mentions) Api 20a, by bioMérieux (3 mentions) Dm2500, by Leica (3 mentions) Tecnai g20 cryo, by Thermo Fisher Scientific (3 mentions) Catalase assays, by bioMérieux (2 mentions) Tecnai g20 transmission electron microscope, by Thermo Fisher Scientific (2 mentions) Bbl dryslide, by BD (2 mentions) Api 50 ch strips, by bioMérieux (2 mentions) Morgani 268d, by Philips (1 mentions) Morgagni 268d, by Philips (1 mentions) Anaerogen, by bioMérieux (1 mentions) Cos agar, by bioMérieux (1 mentions) Petri dish, by bioMérieux (1 mentions) Chapman agar, by Thermo Fisher Scientific (1 mentions) Anaerogen, by Thermo Fisher Scientific (1 mentions) Campygen, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

DM1000 (262 citations) DM1000 microscope (90 citations)

19 protocols using dm1000 photonic microscope

Characterization of Bacillus andreraoultii

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Bacillus andreraoultii strain SIT1^T was observed, after negative colouration, using a Morgani 268D (Philips, Amsterdam, The Netherlands) transmission electron microscope at an operating voltage of 60 kV. The Gram colouration was performed using Color Gram 2 Kit (bioMérieux) and observed by using the DM1000 photonic microscope (Leica Microsystems, Wetzlar, Germany) with a 100× oil-immersion objective lens. The sporulation test was done doing a thermic shock (80°C during 30 minutes). To evaluate the motility of Bacillus andreraoultii, fresh colonies were observed between blades and slats using a DM1000 photonic microscope (Leica) with a 40× objective lens.
API ZYM, API 20 NE and API 50 CH (bioMérieux) gallery systems were used to perform biochemical assays. Oxidase (Becton Dickinson, Franklin Lakes, NJ, USA) and catalase assays (bioMérieux) were done separately. The antibiotic susceptibility was tested using SirScan Discs antibiotics (i2a, Montpellier, France).

Traore S.I., Cimmino T., Lagier J.C., Khelaifia S., Brah S., Michelle C., Caputo A., Diallo B.A., Fournier P.E., Raoult D, & Rolain J.M. (2015). Noncontiguous finished genome sequence and description of Bacillus andreraoultii strain SIT1T sp. nov.. New Microbes and New Infections, 10, 25-35.

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Biochemical Profiling and Microscopy of Marseille-P3135

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In order to biochemically describe strain Marseille‐P3135; different API tests (ZYM, 20A and 50CH, bioMérieux) were used. Sporulation ability of this bacterium was tested by exposing a bacterial suspension for 10 min to a thermal shock at 80°C, and then cultured on COS media. Moreover, the motility of the strain was detected using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) under a 100× objective lens. Also, a bacterial suspension was fixed with a solution of 2.5% glutaraldehyde in 0.1 mol/L cacodylate buffer for more than 1 hr at 4°C for observation under the Morgagni 268D (Philips) transmission electron microscope. Finally, Gram staining results and images were obtained by DM1000 photonic microscope (Leica Microsystems) using a 100× oil‐immersion objective lens.

Bilen M., Fonkou M.D., Tomei E., Armstrong N., Bittar F., Lagier J., Daoud Z., Fournier P., Raoult D, & Cadoret F. (2018). Eggerthella timonensis sp. nov, a new species isolated from the stool sample of a pygmy female. MicrobiologyOpen, 7(5), e00575.

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Characterization of Strain SN6 Bacterium

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Strain SN6 was isolated at first under microaerophilic conditions (CampyGen) at 37°C for 48 hours on Columbia medium supplemented with 5% sheep's blood (COS) agar (bioMérieux) and we also tested its growth under aerobic and anaerobic conditions generated by AnaeroGen (bioMérieux). The minimum and maximum growth temperature ranges (28°C, 37°C, 45°C, 55°C) were determined as well as maximum salinity levels (0–5, 50–75, 100 g/L NaCl). The ability of the strain to grow on media with different pH was also tested. The colonies appeared on day 3 after culture on Columbia agar, and their diameter was measured. Cell morphology, Gram staining and motility were observed in fresh colonies using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40 × objective lens. Sporulation was tested by thermal shock, which consists of exposing the bacterium to a temperature of 60°C for 20 minutes and then watching its growth after 48 hours. Negative staining was carried out with detection Formvar-coated grids placed on a drop of 40 μL of bacterial suspension (after an overnight fixation in glutaraldehyde 2.5%) and incubated at 37°C for 30 minutes, followed by a 10-second incubation in 1% ammonium molybdate. The grids were dried on blotting paper and finally observed using a Tecnai G20 transmission electron microscope (FEI Company, Limeil-Brévannes, France).

Ndongo S., Beye M., Dubourg G., Nguyen T.T., Couderc C., Fabrizio D.P., Fournier P.E., Raoult D, & Angelakis E. (2018). Genome analysis and description of Xanthomonas massiliensis sp. nov., a new species isolated from human faeces. New Microbes and New Infections, 26, 63-72.

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Comprehensive Characterization of Bacterial Strain

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Gram staining and motility were observed from fresh colonies between blades and slats using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40 × objective lens [16] (link). Spore formation was determined by thermal shock (80°C for 20 minutes) and observed under a microscope. Negative staining was carried out with detection formvar-coated grids placed on a drop of 40 μL of bacterial suspension and incubated at 37°C for 30 minutes, followed by a 10-second incubation in 1% ammonium molybdate. The grids were dried on blotting paper and then observed with a Tecnai G20 transmission electron microscope (FEI Company, Limeil-Brévannes, France). We studied the biochemical characteristics of this strain using API 20NE, API ZYM and API 50CH strips according to the manufacturer's instructions (bioMérieux). Oxidase and catalase reactions were determined using a BBL DrySlide (Becton, Le Pont de Claix, France) according to the manufacturer's instructions. The antimicrobial activity test was performed using the disc diffusion method (i2a, Montpellier, France) [17] (link) on Mueller-Hinton agar in a petri dish (bioMérieux).

Ndongo S., Bittar F., Beye M., Robert C., Di Pinto F., Fournier P.E., Raoult D, & Lagier J.C. (2018). ‘Cellulomonas timonensis’ sp. nov., taxonogenomics description of a new bacterial species isolated from human gut. New Microbes and New Infections, 23, 7-16.

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Biochemical Characterization of K. gabonensis

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API ZYM, API 20A and API 50CH (bioMérieux) gallery systems were used to perform biochemical assays. The sporulation test was done with a thermic shock (80°C during 10 minutes). To evaluate the motility of K. gabonensis strain GM4, fresh colonies were observed between blades and slats using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40× objective lens.

Mourembou G., Rathored J., Lekana-Douki J.B., Ndjoyi-Mbiguino A., Fenollar F., Michelle C., Fournier P.E., Raoult D, & Lagier J.C. (2015). Noncontiguous finished genome sequence and description of Kallipyga gabonensis sp. nov.. New Microbes and New Infections, 9, 15-23.

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Morphological Characterization of Bacterial Strain

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To observe the cell morphology, transmission electron microscopy of the strain was performed using a Tecnai G20 Cryo (FEI company, Limeil‐Brevannes, France) at an operating voltage of 60 Kv after negative staining. Gram staining was performed and observed using a photonic microscope Leica DM2500 (Leica Microsystems, Nanterre, France) with a 100X oil‐immersion objective (Atlas & Snyder, 2011). The motility of the strain was assessed by the Hanging Drop method. The slide was examined using a DM1000 photonic microscope (Leica Microsystems) at 40×. Sporulation was tested following a thermic shock at 80°C during 20 min, and the endospore formation was visualized using a Tecnai G20 Cryo transmission electron microscope (FEI company, Limeil‐Brevannes, France) at an operating voltage of 60 Kv after negative staining.

Diop A., Seck E.H., Dubourg G., Armstrong N., Blanc‐Tailleur C., Raoult D, & Fournier P. (2018). Genome sequence and description of Gracilibacillus timonensis sp. nov. strain Marseille‐P2481T, a moderate halophilic bacterium isolated from the human gut microflora. MicrobiologyOpen, 8(2), e00638.

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Microscopy and Motility Analysis of G. massiliensis

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To observe G. massiliensis strain Awa-1^T morphology, transmission electron microscopy was performed after negative staining, using a Tecnai G20 (FEI Company, Limeil-Brevannes, France) at an operating voltage of 60 KV. The gram staining was performed and observed using a photonic microscope Leica DM2500 (Leica Microsystems, Nanterre, France) with a 100X oil-immersion objective. Motility testing was performed by observation of a fresh colony between the blades and slats using DM1000 photonic microscope (Leica Microsystems) at 40x. For the sporulation test, our strain was grown on Chapman agar (Oxoid, Dardilly, France) for 1 week, followed by gram staining and observation for the presence or absence of spores on colonies under the microscope.

Diop A., Khelaifia S., Armstrong N., Labas N., Fournier P.E., Raoult D, & Million M. (2016). Microbial culturomics unravels the halophilic microbiota repertoire of table salt: description of Gracilibacillus massiliensis sp. nov.. Microbial Ecology in Health and Disease, 27, 10.3402/mehd.v27.32049.

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Gram Staining and TEM Imaging of G. massiliensis

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G. massiliensis strain GM3 was observed after negative coloration using a Tecnai G20 (FEI, Limeil-Brevannes, France) transmission electron microscope at an operating voltage of 60 kV. Gram coloration was performed using a Color Gram 2 Kit (bioMérieux). This coloration was observed by using the DM1000 photonic microscope (Leica Microsystems) with a 100× oil-immersion objective lens.

Mourembou G., Rathored J., Ndjoyi-Mbiguino A., Lekana-Douki J.B., Fenollar F., Robert C., Fournier P.E., Raoult D, & Lagier J.C. (2015). Noncontiguous finished genome sequence and description of Gabonia massiliensis gen. nov., sp. nov.. New Microbes and New Infections, 9, 35-44.

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Morphological Analysis of K. gabonensis GM4

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Images of K. gabonensis strain GM4 were obtained using a Tecnai G20 transmission electron microscope (FEI, Limeil-Brevannes, France) at an operating voltage of 60 kV. Gram staining was observed using a DM1000 photonic microscope (Leica Microsystems) with a 100× oil-immersion objective lens.

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Comprehensive Bacterial Characterization

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The API 20A, API ZYM and API 50CH strips (bioMérieux) were used to biochemically characterize strain Marseille-P3237. Sporulation ability was tested after exposing a bacterial suspension to a thermic shock at 80°C for 10 min. Motility was evaluated using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 1000× magnification. Cell morphology was observed using electron microscopy and the following protocol. Bacteria were fixed with 2.5% glutaraldehyde in 0.1 m cacodylate buffer for at least 1 h at 4°C. Then, a drop of cell suspension was deposited for approximately 5 min on glow-discharged formvar carbon film on 400-mesh nickel grids (FCF400-Ni, EMS). The grids were dried on blotting paper and cells were negatively stained for 10 seconds with a solution of 1% ammonium molybdate in filtered water at room temperature. Electron micrographs were acquired with a Tecnai G20 Cryo (FEI) transmission electron microscope operated at 200 keV.

Bilen M., Cadoret F., Richez M., Tomei E., Daoud Z., Raoult D, & Fournier P.E. (2017). Libanicoccus massiliensis gen. nov., sp. nov., a new bacterium isolated from human stool. New Microbes and New Infections, 21, 63-71.

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