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Api 3200 triple quadrupole

Manufactured by AB Sciex
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The API 3200 triple quadrupole is a mass spectrometry instrument designed for quantitative analysis. It features a triple quadrupole configuration, which allows for targeted and sensitive detection of analytes in complex matrices. The core function of the API 3200 is to perform highly selective and sensitive quantitative measurements in a variety of applications.

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Lab products found in correlation

1100 chromatographic system, by AB Sciex (2 mentions)

Close spelling variants of this product

API 3200 (39 citations) API 3200 triple quadrupole mass spectrometer (20 citations)

5 protocols using api 3200 triple quadrupole

1

Quantification of 5-FU in PBMCs

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The test was performed using a HPLC-MS/MS instrument including an Agilent 1100 chromatographic system coupled to an API 3200 triple quadrupole (ABSCIEX, Framingham, MA, USA). [15 (link)] Briefly, freshly prepared peripheral blood mononuclear cells (2.5–3.5 x 106 cells) are incubate at 37°C, with shaking, with a known amount of 5-FU. Cells aliquots are drawn at time 0, 1 h and 2 h, lysed and centrifuged and the concentration of 5-FU in the supernatants is quantified by HPLC-MS/MS. The 5-FUDR is expressed as ng 5-FU/ml/106 cells/min.
Botticelli A., Borro M., Onesti C.E., Strigari L., Gentile G., Cerbelli B., Romiti A., Occhipinti M., Sebastiani C., Lionetto L., Marchetti L., Simmaco M., Marchetti P, & Mazzuca F. (2016). Degradation Rate of 5-Fluorouracil in Metastatic Colorectal Cancer: A New Predictive Outcome Biomarker?. PLoS ONE, 11(9), e0163105.
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2

Liquid Chromatography-Mass Spectrometry Protocol

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Samples were analyzed under the following conditions: UFLC (Shimadzu) AC20 coupled with an API 3200 triple-quadrupole (ABSciex); eluents, (phase A) 95% water, 5% acetonitrile + 0.1% HCOOH, (phase B) 5% water, 95% acetonitrile + 0.1% HCOOH; flow rate, 0.3 mL/min; column, Gemini-Nx 5 μm C18 110A (50 × 2.00 mm) at 35 °C; injection volume, 5 μL. LC–MS/MS analyses were carried out using an ESI(+) interface in multiple reaction monitoring mode.
La Regina G., Bai R., Coluccia A., Famiglini V., Pelliccia S., Passacantilli S., Mazzoccoli C., Ruggieri V., Verrico A., Miele A., Monti L., Nalli M., Alfonsi R., Di Marcotullio L., Gulino A., Ricci B., Soriani A., Santoni A., Caraglia M., Porto S., Pozzo E.D., Martini C., Brancale A., Marinelli L., Novellino E., Vultaggio S., Varasi M., Mercurio C., Bigogno C., Dondio G., Hamel E., Lavia P, & Silvestri R. (2015). New Indole Tubulin Assembly Inhibitors Cause Stable Arrest of Mitotic Progression, Enhanced Stimulation of Natural Killer Cell Cytotoxic Activity, and Repression of Hedgehog-Dependent Cancer. Journal of medicinal chemistry, 58(15), 5789-5807.
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3

UPLC-MS/MS Analysis Conditions

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Samples were analyzed
under the following conditions: UPLC Waters coupled with an API 3200
triple-quadrupole (ABSciex); eluents, (phase A) 95% water, 5% acetonitrile
+ 0.1% HCOOH, (phase B) 5% water, 95% acetonitrile + 0.1% HCOOH; flow
rate, 0.3 mL/min; column, Gemini-Nx 5 μm C18 110A (50 ×
2.00 mm) at 35 °C; injection volume, 10 μL. Source conditions
ESI positive: T 400 °C, Gas 1 30, Gas 2 35, CUR 30, IS 5500,
CAD 5.
Puxeddu M., Wu J., Bai R., D’Ambrosio M., Nalli M., Coluccia A., Manetto S., Ciogli A., Masci D., Urbani A., Fionda C., Coni S., Bordone R., Canettieri G., Bigogno C., Dondio G., Hamel E., Liu T., Silvestri R, & La Regina G. (2022). Induction of Ferroptosis in Glioblastoma and Ovarian Cancers by a New Pyrrole Tubulin Assembly Inhibitor. Journal of Medicinal Chemistry, 65(23), 15805-15818.
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4

HPLC-MS/MS Quantification of 5-FU in PBMCs

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The test was performed as previously reported [23 (link)], using a HPLC-MS/MS instrument (high performance liquid chromatography-mass spectrometry/mass spectrometry) including an Agilent 1100 chromatographic system coupled to an API 3200 triple quadrupole (ABSCIEX, Framingham, MA, USA). Briefly, freshly prepared peripheral blood mononuclear cells (2.5–3.5 × 106 cells) are incubate at 37°C, with shaking, with a known amount of 5-FU. Cells aliquots are drawn at time 0, 1 h and 2 h, lysed and centrifuged and the concentration of 5-FU in the supernatants is quantified by HPLC-MS/MS. The 5-FUDR is expressed as ng 5-FU/ml/106 cells/min.
Mazzuca F., Borro M., Botticelli A., Mazzotti E., Marchetti L., Gentile G., La Torre M., Lionetto L., Simmaco M, & Marchetti P. (2016). Pre-treatment evaluation of 5-fluorouracil degradation rate: association of poor and ultra-rapid metabolism with severe toxicity in a colorectal cancer patients cohort. Oncotarget, 7(15), 20612-20620.
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5

Quantifying 5-FUDR in Blood Cells

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The assay for 5-FUDR has been established in the Sant'Andrea Hospital of Rome as a routine clinical analysis prior to fluorouracil-based chemotherapies and is carried out following medical prescription. The test is performed, as previously reported [29 (link)], using a 5-FUDR assay kit (Eureka srl-Lab Division, Chiravalle, Ancona, Italy) with a HPLC-MS/MS instrument including an Agilent 1100 chromatographic system coupled to an API 3200 triple quadrupole (ABSCIEX, Framingham, MA, USA). Freshly prepared peripheral blood mononuclear cells (2.5-3.5 × 106 cells) are incubate with a known dose of 5-FU at 37°C, with shaking Cells aliquots are analyzed at time 0, 1 h and 2 h. Cells were lysed and centrifuged. 5-FU concentration in the supernatants is quantified by HPLC-MS/MS and the 5-FUDR is expressed as ng 5-FU/ml/106 cells/min. [29 (link)]
Borro M., Botticelli A., Mazzuca F., Onesti E.C., Gentile G., Romiti A., Cerbelli B., Mazzotti E., Marchetti L., Lionetto L., Simmaco M, & Marchetti P. (2016). Pre-treatment assay of 5-fluorouracil degradation rate (5-FUDR) to improve prediction of 5-fluorouracil toxicity in gastro-esophageal cancer. Oncotarget, 8(8), 14050-14057.
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