Anti brf1 2

Manufactured by Cell Signaling Technology

Anti-BRF1/2 is a primary antibody product that specifically recognizes the BRF1 and BRF2 proteins. BRF1 and BRF2 are subunits of the RNA polymerase III transcription initiation complex. This antibody can be used to detect and study the expression and localization of BRF1 and BRF2 in various experimental systems.

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Lab products found in correlation

Anti gfp clone b 2, by Santa Cruz Biotechnology (1 mentions) Anti tubulin dm1a, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit, by Bio-Rad (1 mentions) I block, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Abcam (1 mentions)

2 protocols using anti brf1 2

BRF1/2 Immunoprecipitation and Detection

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Lysates (without crosslinking) and beads were prepared as described in the iCLIP procedure. Immunoprecipitation was performed with 500mg protein as described in the iCLIP procedure. Immunoblotting was performed by standard procedures. 50µg protein of crude lysate was loaded and 10% of the unbound fraction or IP, respectively. Antibodies for immunoblotting were: anti-BRF1/2 (Cell Signaling), anti-GFP (clone B2, Santa Cruz), anti-Tubulin (DM1A, Sigma).

Vogel K.U., Bell L.S., Galloway A., Ahlfors H, & Turner M. (2016). The RNA-binding proteins Zfp36l1 and Zfp36l2 enforce the thymic β-selection checkpoint by limiting DNA damage response signaling and cell cycle progression. Journal of immunology (Baltimore, Md. : 1950), 197(7), 2673-2685.

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Western Blotting of Protein Expression

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Proteins were extracted and Western blotting was performed as described in [26 (link)]. The following primary antibody dilutions were prepared in a PBS solution (0.1 M phosphate buffer was used throughout) containing 0.2% I-Block (Applied Biosystems) and 0.05% Tween-20: anti-BRF1/2 (Cell Signalling Technologies), anti-pan ZFP36-family (SB1/30.13) and anti-β-actin (Abcam). The following secondary antibodies were diluted in a PBS solution containing 0.05% Tween-20: goat anti-rabbit-HRP conjugated (Biorad) or donkey anti-rat-HRP conjugated (Abcam).

Bye-A-Jee H., Pugazhendhi D., Woodhouse S., Brien P., Watson R., Turner M, & Pell J. (2018). The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis. Skeletal Muscle, 8, 37.

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