Eb1089

Calcitriol (1,25-(OH)₂ vitamin D₃) was purchased from Cayman Chemical (Cat #71820) and used at 100 nM unless otherwise noted. Radioimmunoprecipitation assay buffer (RIPA) (Cat #R0278), protease and phosphatase inhibitor cocktails (Cat #P8340, Cat #P5726), and cycloheximide (Cat #01810) were purchased from Sigma Aldrich. FBS was purchased from Seradigm (Cat# 97068-085). IL-2 was purchased from Miltenyi Biotec (Cat #130-097-743). Clarity enhanced chemiluminescence (ECL) reagent (Cat #170-5061) and PVDF membrane and filter paper (Cat #170-4274), were purchased from BioRad. RPMI 1640 (Cat #10-00-CV) and Pierce bicinchoninic acid (BCA) protein assay kit (Cat #PI23225) were purchased from ThermoFisher Scientific. Polyacrylamide gels (4–12%; Cat #NW04125BOX) were purchased from Life Technologies. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethozyphenyl)-2-(4-sulfonphenyl)-2H-tetrazolium (MTS) Cell Proliferation Colorimetric Assay Kit was purchased from BioVision (Cat #K300-2500). EB1089 was purchased from R&D Systems (Cat #3993). 25(OH)D₃ was purchased from Cayman Chemical (Cat #9000683). ON-TARGETplus Pooled Human VDR siRNA (Cat #L-003448-00-0005) and ON-TARGETplus Control Non-Targeting Pool siRNA (Cat #D-001810-01-05) were purchased from Dharmacon.

Calcitriol (1,25-(OH)₂ vitamin D₃) was purchased from Cayman Chemical (Cat #71820). EB1089 was purchased from R&D Systems (Cat #3993). RIPA lysis buffer (Cat #R0278) and protease and phosphatase cocktails (Cat #P8340, Cat #P5726), were all purchased from Sigma Aldrich. All antibodies were purchased from Cell Signaling Technology. FBS was purchased from Seradigm (Cat #97068–085). IL-2 was purchased from Miltenyi Biotec (Cat #130-097-743). Clarity enhanced chemiluminescence (ECL) reagent (Cat #170–5061) and PVDF membrane and paper stacks (Cat #170–4274), were purchased from BioRad. RPMI 1640 (Cat #10–040-CV), Pierce bicinchoninic acid (BCA) protein assay kit (Cat #PI23225), and SuperSignal West Femto Maximum Sensitivity Substrate (Cat #34096) were purchased from ThermoFisher Scientific. ReBlot Plus Strong Antibody Stripping Solution 10X was purchased from Millipore Sigma (Cat #2504). Polyacrylamide gels (4–12%) were purchased from Life Technologies (Cat #NW04125BOX). The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) Cell Proliferation Colorimetric Assay Kit was purchased from BioVision (Cat #K300–2500). Molecular weight markers used in western blotting were SeeBlue Plus2 Pre-Stained Standard (ThermoFisher Scientific) and Flash Protein Ladder #51121659 and #51121660 (WorldWide Life Sciences Division).