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Phenelzine

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Phenelzine is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used as a tool for research and analysis purposes. The core function of Phenelzine is to serve as a reference standard or analytical reagent in various laboratory applications.

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Lab products found in correlation

Clorgyline, by Merck Group (4 mentions) L tryptophan, by Merck Group (3 mentions) Bicinchoninic acid assay bca assay reagents, by Thermo Fisher Scientific (3 mentions) Reserpine, by Merck Group (3 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (2 mentions) Pargyline, by Merck Group (2 mentions) Serotonin hydrochloride, by Merck Group (2 mentions) Imipramine, by Merck Group (2 mentions) Forskolin, by Merck Group (2 mentions) L ascorbic acid, by Merck Group (2 mentions) Nepicastat, by Cayman Chemical (2 mentions) Norepinephrine ne, by Cayman Chemical (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Neon transfection system kit, by Thermo Fisher Scientific (1 mentions) Recombinant tgf β1, by R&D Systems (1 mentions) Ptracer cmv bsd, by Thermo Fisher Scientific (1 mentions) Erlotinib, by Roche (1 mentions) 3h 2 deoxyglucose, by PerkinElmer (1 mentions) Scintillation cocktail, by PerkinElmer (1 mentions)

Spelling variants of this product

Phenelzine sulfate (2 citations) Phenelzine sulfate salt (2 citations)

15 protocols using phenelzine

1

Modulating Breast Cancer Cell Signaling

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All breast cancer cell lines used were sourced from ATCC, except the docetaxel resistant lines which were kind gift of Dr Sikic (Standford University). Cell lines were maintained and cultured in DMEM (Invitrogen) supplemented with 10% FBS, 2 mM L-glutamine, and 1% PSN. MCF-7 cells were stimulated with 1.29 ng/ml phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) and 5 ng/ml recombinant TGF-β1 (R&D Systems) for 60 hours38 (link). For inhibitor experiments, 10 µM, 1 µM and 0.1 µM erlotinib (Roche), 3 mM pargyline (Sigma-Aldrich), 500 µM phenelzine (Sigma-Aldrich), 1 µM BIM (Calbiochem), and 1 µM C27 (SYNthesis Med Chem) were used. All inhibitors were pre-incubated for 24 hours before stimulating cells. Forward transfection reactions were performed with 40 nM human siRNA (sc-60970) and mock siRNA (sc-36869) (Santa Cruz Biotechnology) using Lipofectamine 2000 (Invitrogen). Vector transfections were performed with 15 µg of either vector only plasmid (pTracer-CMV/BSD, Thermo Scientific), LSD1-WT, or LSD1-Mut (Serine-111 to Alanine) using the NEONTM Transfection System kit (MPK5000; Invitrogen).
Boulding T., McCuaig R.D., Tan A., Hardy K., Wu F., Dunn J., Kalimutho M., Sutton C.R., Forwood J.K., Bert A.G., Goodall G.J., Malik L., Yip D., Dahlstrom J.E., Zafar A., Khanna K.K, & Rao S. (2018). LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Scientific Reports, 8, 73.
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2

Neurochemical Assessment of Biochemical Interactions

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Opipramol (4-[3-(5H-dibenz[b,f]-azepine-5-yl)-propyl]-1-piperazine-ethanol dihydrochloride), haloperidol, risperidone, phenelzine, pargyline, harmine, tyramine, benzylamine, semicarbazide, cytochalasin B, fatty acid-free bovine serum albumin, hydrogen peroxide, horseradish peroxidase, isoprenaline, cytochalasin B, and routinely used chemicals were obtained from Sigma-Aldrich-Merck (St. Quentin Fallavier, France), unless otherwise specified. Amplex Red was provided by InterChim (Montluçon, France). [3H]-2-Deoxyglucose, [14C]-benzylamine, and scintillation cocktail were purchased from PerkinElmer (Boston, MA, USA). Different [14C]-tyramine batches were either from PerkinElmer or Sigma. Olanzapine and ziprazidone were generous gifts from Prof. Renaud de Beaurepaire (Hosp. Paul-Guiraud, Villejuif, France).
Carpéné C., Les F., Mercader J., Gomez-Zorita S., Grolleau J.L., Boulet N., Fontaine J., Iglesias-Osma M.C, & Garcia-Barrado M.J. (2020). Opipramol Inhibits Lipolysis in Human Adipocytes without Altering Glucose Uptake and Differently from Antipsychotic and Antidepressant Drugs with Adverse Effects on Body Weight Control. Pharmaceuticals, 13(3), 41.
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3

Methamphetamine-Induced Neurodegeneration in Mice

Cited 3 times
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The male and female mice began in vivo treatments at approximately 8 weeks of age. The subjects were administered saline (10 mL/kg; General Laboratory Products) or (+)-methamphetamine hydrochloride (meth, 5 mg/kg; Sigma-Aldrich, St. Louis, MO, USA) for 14, 21, or 28 consecutive days in the home cage. To test whether monoamine oxidase (MAO) inhibition prevents degeneration, the irreversible MAO-A/B inhibitor phenelzine (20 mg/kg; Sigma-Aldrich) was administered as a 30-min pretreatment prior to each meth injection. Meth and phenelzine were dissolved in sterile saline, and all of the injections were intraperitoneal. The dose of meth and 28-day duration were based on our prior studies demonstrating meth-induced degeneration of SNc and LC neurons in male mice [36 (link),37 (link),42 (link)]. The dose of phenelzine (20 mg/kg) was chosen based on it being a behaviorally relevant dose in mice [106 (link)].
Pilski A, & Graves S.M. (2023). Repeated Methamphetamine Administration Results in Axon Loss Prior to Somatic Loss of Substantia Nigra Pars Compacta and Locus Coeruleus Neurons in Male but Not Female Mice. International Journal of Molecular Sciences, 24(17), 13039.
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4

Serotonin and Forskolin Protocol

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Serotonin hydrochloride, L-Tryptophan, and phenelzine (MAO inhibitor) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Forskolin (proliferation-activating agent) was obtained from Scintila, s.r.o. (Jihlava, CZ). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Waltham, MA, United States). All other chemicals were of analytical grade.
Karahoda R., Abad C., Horackova H., Kastner P., Zaugg J., Cerveny L., Kucera R., Albrecht C, & Staud F. (2020). Dynamics of Tryptophan Metabolic Pathways in Human Placenta and Placental-Derived Cells: Effect of Gestation Age and Trophoblast Differentiation. Frontiers in Cell and Developmental Biology, 8, 574034.
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5

Antidepressant Effects in Mice Model

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Mice received 21 days of four different types of antidepressants as described previously (Sakata et al. 2013b (link)). Briefly, mice received a single intraperitoneal (i.p.) injection per day with saline or with one of the four different classes of antidepressant: fluoxetine (10 mg/kg, Mallinckrodt, Inc., Hazelwood, MO), phenelzine (10 mg/kg, Sigma, St. Louis, MO), duloxetine (20 mg/kg, Eli Lilly, Indianapolis, IN), and imipramine (10 mg/kg, Sigma). Animals were sacrificed 3 h after the last injection. The method of drug administration (i.p.), the dosage, and the sacrifice timing were decided based on previous studies (Dwivedi et al. 2006 (link), Khundakar & Zetterstrom 2006 (link), Molteni et al. 2009 (link), Mannari et al. 2008 (link), Tsankova et al. 2006 (link), Nibuya et al. 1995 (link)).
Sakata K, & Overacre A.E. (2017). Promoter IV-BDNF deficiency disturbs cholinergic gene expression of CHRNA5, CHRM2, and CHRM5: effects of drug and environmental treatments. Journal of neurochemistry, 143(1), 49-64.
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6

NMR and Mass Spectrometry Analysis

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A Bruker model AMX 500 NMR and 400 NMR spectrometers operating on a standard pulse system were used to acquire 1H and 13C NMR and 2D spectra. The instruments ran at 500 and 400 MHz for 1H while they ran at 125 and 100 MHz for 13C. CDCl3, DMSO-d6, and acetone-d6 were used as NMR solvents, and TMS was used as an internal standard. ESI-MS data were recorded on Thermo Orbitrap Fusion (Thermo Scientific). Samples were analyzed in the negative mode of ionization. Samples were directly infused at 3 uL/min. Mass was analyzed in Orbitrap (mass error on the instrument <2 ppm). ESI-MS data were obtained on a Micromass Q-Tof micromass spectrometer. FTMS-ESI was analyzed on Thermo Orbitrap Fusion (Thermo Scientific). The sample was analyzed in the negative mode of ionization. Mass was analyzed in Orbitrap (mass error on the instrument <2 ppm). TLC was performed on precoated silica gel GF254 plates and Column Chromatography was performed on silica gel (200–300 mesh) and Sorbadex-LH20 (Sorbent Technologies, Atlanta, GA, USA). The recombinant human monoamine oxidase-A and monoamine oxidase-B enzymes were obtained from BD Biosciences (Bedford, MA, USA). Kynuramine, clorgyline, phenelzine, deprenyl, and DMSO were procured from Sigma Chemical Company (St. Louis, MO, USA).
Orazbekov Y., Ibrahim M.A., Mombekov S., Srivedavyasasri R., Datkhayev U., Makhatov B., Chaurasiya N.D., Tekwani B.L, & Ross S.A. (2018). Isolation and Biological Evaluation of Prenylated Flavonoids from Maclura pomifera. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 1370368.
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7

Quantification of GFP Expression in HIV-Latent Cells

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Quantification of GFP expression was performed by flow cytometry analysis using a LSR Fortessa instrument, the FACSDiva software (BD, NJ) for data collection, and the WinList 3D software for data analysis. Prior to analysis, CHME-5/HIV and HC69 cells (adherent) were trypsinized, collected, and resuspended in 300 μL of cold PBS, while the suspension cells 2D10 and HA3 were centrifuged, and pellets resuspended in 300 μL of PBS. Drug treatments of HIV-latently infected CHME-5/HIV, HC69, HA3, and 2D10 cells were typical for 16 h, with the following concentrations: 4 μM BIX01294 (Sigma-Aldrich B9311), 3 μM UNC0638 (Sigma-Aldrich U4885), 500 μM phenelzine (Sigma-Aldrich P6777), 100 μM M-30 (Sigma-Aldrich SML0128), 100 μM RN-1 (Tocris 4977), 100 μM GSK-LSD1 (Sigma-Aldrich SML1072), and 2 μM SP-2509 (Cayman Chemical 15487).
Llewellyn G.N., Alvarez-Carbonell D., Chateau M., Karn J, & Cannon P.M. (2017). HIV-1 infection of microglial cells in a reconstituted humanized mouse model and identification of compounds that selectively reverse HIV latency. Journal of Neurovirology, 24(2), 192-203.
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8

Radioligand Binding Assay Protocol

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[3H]5-hydroxytryptamine ([3H]5-HT), 80 Ci mmol−1 and [3H]dihydroalprenolol, levo-[propyl-1,2,3–3H] hydrochloride, 80 Ci mmol−1 were purchased from American Radiolabeled Chemicals, Inc. Unlabeled serotonin hydrochloride, L-tryptophan, paroxetine hydrochloride, citalopram hydrobromide, venlafaxine hydrochloride, cimetidine hydrochloride, reserpine, corticosterone, cortisol and phenelzine were purchased from Sigma-Aldrich. All other chemicals were of analytical grade. Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific. Tri Reagent solution was obtained from the Molecular Research Centre.
Karahoda R., Horackova H., Kastner P., Matthios A., Cerveny L., Kucera R., Kacerovsky M., Tebbens J.D., Bonnin A., Abad C, & Staud F. (2020). Serotonin homeostasis in the materno-foetal interface at term: Role of transporters (SERT/SLC6A4 and OCT3/SLC22A3) and monoamine oxidase A (MAO-A) in uptake and degradation of serotonin by human and rat term placenta. Acta physiologica (Oxford, England), 229(4), e13478.
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9

Phenelzine Inhibits HIV in Glial Cells

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Glial cells (5 × 105) isolated from HIV-infected humanized mice were cultured in DMEM-F/12 (ThermoFisher Scientific), 10% FBS and penicillin/streptomycin, with or without 5 μM phenelzine (Sigma). After 2 days, HIV was quantified from the supernatants via RNA extraction and qRT-PCR, as described above.
Llewellyn G.N., Alvarez-Carbonell D., Chateau M., Karn J, & Cannon P.M. (2017). HIV-1 infection of microglial cells in a reconstituted humanized mouse model and identification of compounds that selectively reverse HIV latency. Journal of Neurovirology, 24(2), 192-203.
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10

Prostate Cancer Cell Lines and Reagents

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Human prostate cancer cell lines LNCaP and 22RV1 were purchased from American Type Culture Collection (Manassas, VA). C4–2B28 (link) cells were kindly provided and authenticated by Dr. Leland W. Chung, Cedars-Sinai Medical Center (Los Angeles, CA). Cell culture media and serum were obtained from Invitrogen Life Technologies (Carlsbad, CA). Enzalutamide (MDV-3100) was purchased from Selleckchem (Houston, TX) and R1881, Clorgyline and Phenelzine were obtained from Sigma-Aldrich (St. Louis, MO). Antibodies against different proteins were obtained from Santa Cruz Biotechnologies Inc. (Santa Cruz, CA), Cell signaling technology Inc. (Beverley, MA) or Abcam (Cambridge, MA). HRP Conjugated anti-mouse and anti-rabbit IgG were purchased from Cell signaling technology Inc. and Super signal chemiluminescence substrates were purchased from Thermo Fisher Scientific (Waltham, MA). All other reagents were obtained from VWR (Radnor, PA).
Gaur S., Gross M.E., Liao C.P., Qian B, & Shih J.C. (2019). Effect of Monoamine oxidase A (MAOA) inhibitors on androgen-sensitive and castration-resistant prostate cancer cells. The Prostate, 79(6), 667-677.
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