Amt 2k digital camera

Manufactured by Advanced Microscopy Techniques

Sourced in Morocco, United States

The AMT 2k digital camera is a high-resolution imaging device designed for use in advanced microscopy applications. It features a 2048 x 2048 pixel sensor, providing detailed, high-quality images. The camera is capable of capturing images with a wide dynamic range and low noise levels, making it suitable for a variety of microscopy techniques.

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Lab products found in correlation

Polybed 812 epoxy resin, by Polysciences (4 mentions) Jem 1011 transmission electron microscope, by Advanced Microscopy Techniques (2 mentions) Rabbit anti bip, by Abcam (2 mentions) Ultracut 7, by Leica (2 mentions) Lr white resin, by Electron Microscopy Sciences (2 mentions) Jem 1011 transmission electron microscope, by JEOL (2 mentions) 1400 transmission electron microscope, by JEOL (1 mentions) Aqueous uranyl acetate, by Electron Microscopy Sciences (1 mentions) Lx 112, by Electron Microscopy Sciences (1 mentions) Diatome diamond knife, by Electron Microscopy Sciences (1 mentions) Illustrator cc, by Adobe (1 mentions) Embed 812 resin, by Electron Microscopy Sciences (1 mentions) Em uc6 ultramicrotome, by Leica (1 mentions) 0.1 m sorensen s buffer, by Electron Microscopy Sciences (1 mentions) Jem 1011 tem, by JEOL (1 mentions) Potassium ferricyanide, by Thermo Fisher Scientific (1 mentions) Sodium borate, by Thermo Fisher Scientific (1 mentions) Osmium tetroxide, by Electron Microscopy Sciences (1 mentions) Toluidine blue o, by Thermo Fisher Scientific (1 mentions) Reichart ultracut, by Leica (1 mentions)

21 protocols using amt 2k digital camera

Transmission Electron Microscopy Specimen Preparation

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The specimens were fixed in cold 2.5% glutaraldehyde in PBS, pH 7.3. The specimens were rinsed in PBS, post-fixed in 1% osmium tetroxide with 1% potassium ferricyanide, dehydrated through a graded series of ethanol (30–90%) and embedded in Poly/Bed® 812 (Luft formulations). Semi-thin (300 nm) sections were cut on a Leica Reichart Ultracut (Leica Microsystems, Buffalo Grove, IL), stained with 0.5% Toluidine Blue in 1% sodium borate and examined under the light microscope. Ultrathin Sects. (65 nm) were stained with 2% uranyl acetate and Reynold’s lead citrate and examined on JEOL 1400 transmission electron microscope (JEOL Peabody, MA) (NIH grant #1S10RR016236-01, Simon Watkins) with a side mount AMT 2 k digital camera (Advanced Microscopy Techniques, Danvers, MA).

Heiman P., Mohsen A.W., Karunanidhi A., St Croix C., Watkins S., Koppes E., Haas R., Vockley J, & Ghaloul-Gonzalez L. (2022). Mitochondrial dysfunction associated with TANGO2 deficiency. Scientific Reports, 12, 3045.

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Transmission Electron Microscopy Preparation

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Fixed samples were osmicated 1–2 h with 1.5% (w/v) reduced OsO₄ in 100 mm cacodylate, pH 7.4, washed several times with distilled water, and then block stained overnight at 4 °C in 0.5% (w/v) aqueous uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Tissues were dehydrated in a graded series of ethanol, embedded in the epoxy resin LX-112 (Electron Microscopy Sciences), and sections (pale gold in color) were cut with a Diatome diamond knife (Electron Microscopy Sciences). Sections were counterstained with uranyl acetate and lead citrate and viewed on a JEOL 1011 transmission EM with a side mount AMT 2K digital camera (Advanced Microscopy Techniques, Danvers, MA). Images were imported into Photoshop CC (Adobe, San Jose, CA), adjusted for brightness and contrast, and then assembled in Adobe Illustrator CC. All of the EM studies were performed using an n = 3 for experimental and control. While our study does not permit the quantification of the frequency of each phenotype (mitobodies, and junctional defects), these abnormalities were readily observed in every mutant analyzed. None of these features were observed in any of the controls. The degree to which each phenotype represents the displayed image in the figures is described below for the specific image.

Liu C., Tate T., Batourina E., Truschel S.T., Potter S., Adam M., Xiang T., Picard M., Reiley M., Schneider K., Tamargo M., Lu C., Chen X., He J., Kim H, & Mendelsohn C.L. (2019). Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells. Nature Communications, 10, 4589.

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Ultrastructural Analysis of Peri-Infarct Cortex

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TEM was performed as desribed previously (Begum et al., 2018 (link)). Briefly, mice were transcardially perfused with PBS followed by fixation with 2.5% glutaraldehyde for 24 h. After fixation, the brain was sectioned into 1 mm thick slices and post fixed in 2.5% glutaraldehyde in PBS. Under a dissection microscope, tissue punches were taken to capture the IL and CL peri-infarct areas in the cortex. Tissues were washed three times in PBS then, post-fixed in 1% osmium tetroxide with 1% potassium ferricyanide for 1 h. Following three additional PBS washes, the pellet was dehydrated through a graded series of 30-100% ethanol, 100% propylene oxide and then infiltrated in 1:1 mixture of propylene oxide: Polybed 812 epoxy resin for 1 h. After several changes of 100% resin over 24 hrs, pellet was embedded in a final change of resin, cured at 37°C overnight, followed by additional hardening at 65°C for two more days. Ultrathin (70 nm) sections were collected on 200 mesh copper grids, stained with 2% uranyl acetate in 50% methanol for 10 minutes, followed by 1% lead citrate for 7 min. Sections were imaged using a JEOL JEM 1011 transmission electron microscope (Peabody, MA) at 80 kV fitted with a side mount AMT 2k digital camera (Advanced Microscopy Techniques, Danvers, MA).

Song S., Huang H., Guan X., Fiesler V., H. Bhuiyan M.I., Liu R., Jalali S., Hasan M.N., Tai A.K., Chattopadhyay A., Chaparala S., Sun M., Stolz D.B., He P., Agalliu D., Sun D, & Begum G. (2020). Activation of endothelial Wnt/β-catenin signaling by protective astrocytes repairs BBB damage in ischemic stroke. Progress in neurobiology, 199, 101963.

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Ultrastructural Analysis of ER Morphology

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Control HCT116 cells, and those treated with sulindac sulfide were analyzed for ER morphology by TEM as previously described [50 (link)]. For immunogold TEM, cells were fixed in cold 2% paraformaldehyde in 0.01M PBS (pH 7.4), rinsed in PBS, dehydrated through a graded series of ethanol, and infiltrated with and embedded in LR White resin (Electron Microscopy Sciences). Semi-thin (300 nm) sections were obtained on a Leica Ultracut 7, stained with 0.5% Toluidine Blue in 1% sodium borate, and examined under the light microscope to determine specific areas. Ultrathin sections (65 nm) were picked up on 100 mesh nickel grids, labeled with 1:100 dilution of rabbit anti-BiP (Abcam) overnight at 4°C, and then labeled with a 6 nm goat anti-rabbit colloidal gold conjugated secondary at a dilution of 1:10 at room temperature for 1 hr. After rinsing in PBS and dH₂O, the sections were counterstained with 2% uranyl acetate and examined on JEOL 1011 transmission electron microscope with a side mount AMT 2k digital camera (Advanced Microscopy Techniques).

Fletcher R., Tong J., Risnik D., Leibowitz B., Wang Y.J., Concha-Benavente F., DeLiberty J.M., Stolz D.B., Pai R.K., Ferris R.L., Schoen R.E., Yu J, & Zhang L. (2021). Non-steroidal anti-inflammatory drugs induce immunogenic cell death in suppressing colorectal tumorigenesis. Oncogene, 40(11), 2035-2050.

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Ultrastructural Analysis of Bladder Aging

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Urinary bladder sections from each group (young, aged, and aged + 8-AG treatment) was fixed in cold 2.5% glutaraldehyde in 0.01M PBS. The specimens were rinsed in PBS, post-fixed in 1% osmium tetroxide with 1% potassium ferricyanide, rinsed in PBS, dehydrated through a graded series of ethanol and propylene oxide solutions, and embedded in Poly/Bed 812 (Luft formulations). Semithin (300 nm) sections were cut on a Leica Reichart Ultracut, stained with 0.5% Toluidine Blue in 1% sodium borate, and examined under the light microscope. Ultrathin sections (65 nm) were stained with uranyl acetate and Reynold’s lead citrate and examined on a JEOL 1400 transmission electron microscope with a side mount AMT 2k digital camera (Advanced Microscopy Techniques).

Birder L.A., Wolf-Johnston A., Wein A.J., Cheng F., Grove-Sullivan M., Kanai A.J., Watson A.M., Stoltz D., Watkins S.C., Robertson A.M., Newman D., Dmochowski R.R, & Jackson E.K. (2020). Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions. JCI Insight, 5(20), e140109.

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Ultrastructural analysis of NG-exposed ecto-cervical tissue

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Human Ecto-cervical tissues were exposed to NG for 24 hours and fixed in 2.5 % glutaraldehyde for 1 hour at room temperature. The biopsies were washed with 0.1 M PBS (pH 7.4) a few times and then the tissues were incubated in 1% OsO4 in 0.1 M PBS for 60 minutes. This was again washed thoroughly 3 times with 0.1 M PBS for 15 minutes each and processed using a protocol from the Center for Biologic imaging (CBI) at the University Of Pittsburgh as previously described^{43 (link)}. SEM images were acquired using a (JEOL JEM 1011 TEM (Peabody, MA) at 80kV fitted with a side-mount AMT 2k digital camera (Advanced Microscopy Techniques, Danvers, MA).

Sanyal A., Shen C., Ding M., Reinhart T.A., Chen Y., Sankapal S, & Gupta P. (2019). Neisseria gonorrhoeae uses cellular proteins CXCL10 and IL8 to enhance HIV‐1 transmission across cervical mucosa. American Journal of Reproductive Immunology, 81(6), e13111.

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Electron Microscopy Visualization of Activated T Cells

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Variously activated CD8⁺ T cells from pMel-1 mice were fixed with 4% glutaraldehyde solution and stained for electron microscopy. Fixed cells were post fixed in 1% osmium tetroxide (OsO₄) in PBS, rinsed in PBS then embedded in 2% agarose. The cells were then dehydrated through graded ethanol to propylene oxide and infiltrated with 1:1 propylene oxide:Embed 812 resin (Electron Microscopy Sciences) overnight, 1:3 propylene oxide:Embed 812 resin for 4 h and, finally, 100% Embed 812 resin overnight. The beam capsules were cured at 70 °C for 48 h before sectioning. Thin sections (90 nm) were cut with a Leica EM UC6 ultramicrotome (Leica Microsystems) on a diamond knife, placed onto a 300 mesh copper grid and stained using 2% uranyl acetate and Reynold’s lead citrate. Samples were evaluated at 80 kV using a JEOL JM-1230 transmission electron microscope and images collected with an AMT 2K digital camera (Advanced Microscopy Techniques).

You say statistics, I say stastistics. (2001). CMAJ: Canadian Medical Association Journal, 164(9), 1280.

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Ultrastructural Examination of F. tularensis

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Wild type F. tularensis LVS was cultured in CDM for 24, 48, 96, and 336-h in triplicate. At each time point, 1 ml of cells was pelleted from the same flask and was fixed for 1 h in 2.5 % glutaraldehyde in 0.1 M Sorensen’s buffer, pH 7.2 (Electron Microscopy Sciences) and then washed twice in 0.1 M Sorensen’s buffer, pH 7.2 (Electron Microscopy Sciences) and stored at 4°C. Washed bacteria were fixed in 1% OsO₄, 1% K₃Fe (CN)₆ for 1 h. Following 3 additional PBS washes, the pellet was dehydrated through a graded series of 30–100% ethanol, 100% propylene oxide then infiltrated in 1:1 mixture of propylene oxide: Polybed 812 epoxy resin (Polysciences, Warrington, PA, USA) for 1 h. After several changes of 100% resin over 24 h, pellet was embedded in a final change of resin, cured at 37°C overnight, followed by additional hardening at 65°C for two more days. Ultrathin (60–70 nm) sections were collected on 200 mesh copper grids, stained with 2% uranyl acetate in 50% methanol for 10 min, followed by 1% lead citrate for 7 min. Sections were imaged using a JEOL FLASH JEM 1400 transmission electron microscope (Peabody, MA) at 80 kV fitted with a bottom mount AMT 2k digital camera (Advanced Microscopy Techniques, Danvers, MA, USA).

Cantlay S., Garrison N.L., Patterson R., Wagner K., Kirk Z., Fan J., Primerano D.A., Sullivan M.L., Franks J.M., Stolz D.B, & Horzempa J. (2024). Phenotypic and transcriptional characterization of F. tularensis LVS during transition into a viable but non-culturable state. Frontiers in Microbiology, 15, 1347488.

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Ultrastructural Analysis of CpG-Treated Cells

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Cells (5.0 × 10⁴/mL) were seeded in cell culture bottles before being treated on the following day with 10 μg/mL of CpG ODN107 for 12 h, followed by treatment with or without irradiation. After incubation for a further 24 h, cells were collected and fixed in cold 2.5% glutaraldehyde in phosphate buffered saline (PBS). The specimens were post-fixed in 1% osmium tetroxide with 0.1% potassium ferricyanide, dehydrated through a graded series of ethanol (30–90%), and embedded in Epon. Ultrathin sections (65 nm) were stained with 2% uranyl acetate and Reynold’s lead citrate, and imaged using a JEOL JEM-1011 TEM at 80 KV. Images were captured using a side-mount AMT 2k digital camera (Advanced Microscopy Techniques, Danvers, MA, USA).

Li X., Cen Y., Cai Y., Liu T., Liu H., Cao G., Liu D., Li B., Peng W., Zou J., Pang X., Zheng J, & Zhou H. (2016). TLR9-ERK-mTOR signaling is critical for autophagic cell death induced by CpG oligodeoxynucleotide 107 combined with irradiation in glioma cells. Scientific Reports, 6, 27104.

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Ultrastructural Analysis of Mitochondria

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Cells were fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, 111–30-8) in 0.01 M PBS (Fisher), pH 7.3. Samples were rinsed in PBS, post-fixed in 1% osmium tetroxide (Electron Microscopy Sciences) with 1% potassium ferricyanide, (Fisher), rinsed in PBS, dehydrated through a graded series of ethanol, and embedded in Poly/Bed^® 812 (Luft formulations). Semi-thin (300 nm) sections were cut on a Reichart Ultracut (Leica Microsystems), stained with 0.5% Toluidine Blue O (Fisher) in 1% sodium borate (Fisher) and examined under the light microscope. Ultrathin sections (65 nm) were stained with 2% uranyl acetate (Electron Microscopy Science) and Reynold’s lead citrate (Lead Nitrate, Sodium Citrate and Sodium Hydroxide, Fisher) and examined on JEOL 1400 Plus transmission electron microscope with a side mount AMT 2k digital camera (Advanced Microscopy Techniques). For morphometric analysis of mitochondria by transmission electron microscopy, mitochondrial area, mitochondrial length (major axis), cristae number and cristae volume density per mitochondria was quantified and analyzed as described⁷⁶ using ImageJ. At least 40–50 mitochondria per biological replicate over 3 experimental replicates were analyzed.

Javed Z., Shin D.H., Pan W., White S.R., Kim Y.S., Elhaw A.T., Kamlapurkar S., Cheng Y.Y., Benson J.C., Abdelnaby A.E., Phaëton R., Wang H.G., Yang S., Sullivan M.L., St.Croix C.M., Watkins S.C., Mullett S.J., Gelhaus S.L., Lee N., Coffman L.G., Aird K.M., Trebak M., Mythreye K., Walter V, & Hempel N. (2024). Alternative splice variants of the mitochondrial fission protein DNM1L/Drp1 regulate mitochondrial dynamics and tumor progression in ovarian cancer. bioRxiv.

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