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Mem non essential amino acids neaas

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MEM Non-Essential Amino Acids (NEAAs) is a laboratory product manufactured by Thermo Fisher Scientific. It is a solution containing a mixture of non-essential amino acids that are commonly used in cell culture media to support the growth and development of cells in vitro.

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Lab products found in correlation

Hepes, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Macs gmp expact treg beads, by Miltenyi Biotec (1 mentions) Fetal bovine serum (fbs), by Genesee Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) Atcc cl 101, by American Type Culture Collection (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Ketamine, by Merck Group (1 mentions) Dextromethorphan, by Merck Group (1 mentions) Blasticidin, by Thermo Fisher Scientific (1 mentions) Fluo 4, by Thermo Fisher Scientific (1 mentions) Hygromycin b, by Thermo Fisher Scientific (1 mentions) Zeocin, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium f12 dmem f12, by Thermo Fisher Scientific (1 mentions) Tryple enzyme, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Non-essential amino acid Non-essential amino acid (NEAA; MEM non-essential amino acids Essential amino acids MEM amino acids MEM NEAA Amino acids

4 protocols using mem non essential amino acids neaas

1

Ex Vivo Expansion of Regulatory T Cells

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Following FACS isolation, cells were expanded for 14 days ex vivo (13 (link)). In brief, Tregs were cultured in complete RPMI media (cRPMI; RPMI 1640 media Phenol Red w/o L-Glutamine (Lonza, Basel, CH-BS, Switzerland), 5mM HEPES (Gibco, Waltham, MA, USA), 5 mM MEM Non-Essential Amino Acids (NEAAs; Gibco), 2mM Glutamax (Gibco), 50 µg/mL penicillin (Gibco), 50 µg/mL streptomycin (Gibco), 20 mM sodium pyruvate (Gibco), 50 mM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA), 20 mM sodium hydroxide (Sigma-Aldrich) and 10% FBS (Genesee Scientific, El Cajon, CA, USA)) with Teceleukin recombinant human IL-2 (rhIL-2; Roche, Basel, CH-BS, Switzerland) at 300 IU/mL, with media and rhIL-2 being replaced every 3-4 days. Tregs were stimulated using MACS® GMP ExpAct Treg Beads (Miltenyi Biotec, Bergisch Gladbach, NW, Germany) at a 4:1 bead:cell ratio. Beads were replaced at day seven, and cells were expanded through day 14.
Brown M.E., Peters L.D., Hanbali S.R., Arnoletti J.M., Sachs L.K., Nguyen K.Q., Carpenter E.B., Seay H.R., Fuhrman C.A., Posgai A.L., Shapiro M.R, & Brusko T.M. (2022). Human CD4+CD25+CD226- Tregs Demonstrate Increased Purity, Lineage Stability, and Suppressive Capacity Versus CD4+CD25+CD127lo/- Tregs for Adoptive Cell Therapy. Frontiers in Immunology, 13, 873560.
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2

Porcine Kidney Cell Culture for PDCoV Isolation

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LLC porcine kidney
(LLC-PK) cells were purchased from the ATCC (ATCC CL-101) and cultured
at 37 °C with 5% CO2 in the modified Eagle medium
(MEM) (Invitrogen, Carlsbad, USA) containing 10% heat-inactivated
fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 1% MEM nonessential
amino acids (NEAAs) (Gibco, Grand Island, NY, USA), 1% HEPES (Gibco,
Grand Island, NY, USA), and 1% antibiotic-antimycotic (Gibco, Grand
Island, NY, USA). The PDCoV strain CH/XJYN/2016 (GenBank accession
number: MN064712) was isolated from intestinal samples of a suckling
piglet experiencing acute diarrhea using LLC-PK cells in our laboratory.
Gao X., Zhang L., Zhou P., Zhang Y., Wei Y., Wang Y, & Liu X. (2020). Tandem Mass Tag-Based Quantitative Proteome Analysis of Porcine Deltacoronavirus (PDCoV)-Infected LLC Porcine Kidney Cells. ACS Omega, 5(35), 21979-21987.
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3

Antagonist-based Screening of Neuroprotective Compounds

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All chemicals were of analytical grade. Dextromethorphan and (±)-ketamine were from Merck Sigma-Aldrich (Milan, Italy). Memantine and (+)-MK-801 were from Bio-Techne/Tocris (Milan, Italy). REL-1017 (dextromethadone HCl) was from SpecGx (Webster Groves, MO, USA).
Cell culture consumables were from Gibco Life Technologies (Milan, Italy), including Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12; Cat # 21331), heat-inactivated fetal bovine serum (FBS; Cat # 10500), penicillin-streptomycin (Cat # 15140), L-glutamine (Cat # 25030), MEM nonessential amino acids (NEAAs; Cat # 11140), blasticidin (Cat # A11139), G418 (Cat # 10131), Zeocin™ (Cat # R250), and TrypLE™ enzyme (Cat # 12604). Hygromycin B (Cat# 10687) and Fluo-4 (F14202) were from Invitrogen (Waltham, MA, USA).
Bettini E., Stahl S.M., De Martin S., Mattarei A., Sgrignani J., Carignani C., Nola S., Locatelli P., Pappagallo M., Inturrisi C.E., Bifari F., Cavalli A., Alimonti A., Pani L., Fava M., Traversa S., Folli F, & Manfredi P.L. (2022). Pharmacological Comparative Characterization of REL-1017 (Esmethadone-HCl) and Other NMDAR Channel Blockers in Human Heterodimeric N-Methyl-D-Aspartate Receptors. Pharmaceuticals, 15(8), 997.
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4

Differentiation of Primary Human Monocyte-derived Macrophages

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To generate primary human monocyte-derived MΦs, CD14+ monocytes were cultured in 25 cm2 tissue culture flasks (1 × 106 cells per mL; Falcon/Corning) in Roswell Park Memorial Institute (RPMI) medium -1640, w/L-glutamine (Lonza, Bend, OR, USA) media, supplemented with 1 mM of sodium pyruvate (Lonza), 1X MEM non-essential amino acids (NEAAs; Gibco, ThermoFisher Scientific, USA), 10% (v/v) fetal bovine serum (FBS; ATCC, Manassas, VA, USA), and 50 ng/mL recombinant human M-CSF (rHU-MCSF; PeproTech, Rocky Hill, NJ, USA) for 6 d. Media and cytokines were replenished every 3 d.
Fuchs A.L., Schiller S.M., Keegan W.J., Ammons M.C., Eilers B., Tripet B, & Copié V. (2019). Quantitative 1H NMR Metabolomics Reveal Distinct Metabolic Adaptations in Human Macrophages Following Differential Activation. Metabolites, 9(11), 248.
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